Inhibition of Otopathogenic Biofilms by Organoselenium-Coated Tympanostomy Tubes

Wang, James C.; Tran, Phat L.; Hanes, Rob; Cordero, Joehassin; Marchbanks, John; Reid, Ted W.; Colmer-Hamood, Jane A.; Hamood, Abdul N.

doi:10.1001/jamaoto.2013.4690

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…The microtiter plate culture assay was utilized to develop the biofilms as previously described [15,16]. Briefly, aliquots of overnight cultures of the tested strain in LB broth were harvested, washed in 1 mL PBS, and diluted in PBS to an OD 600 of 0.02.…”

Section: Biofilm Developmentmentioning

confidence: 99%

Next-Science: A Novel Antimicrobial Agent that Inhibits Biofilm Development by Escherichia coli Clinical Isolates on Urinary Tract Catheters

Siddiqui¹,

Kruczek²,

Colmer-Hamood³

et al. 2017

J Med Microb Diagn

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Section: Biofilm Developmentmentioning

confidence: 99%

Next-Science: A Novel Antimicrobial Agent that Inhibits Biofilm Development by Escherichia coli Clinical Isolates on Urinary Tract Catheters

Siddiqui¹,

Kruczek²,

Colmer-Hamood³

et al. 2017

J Med Microb Diagn

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“…•-) in a continuous process in which Se donates an electron to oxygen and acquires one from sulfur compounds (such as glutathione) that are present in all body fluids [1]. Recently, it has been shown that organo-selenium can be attached to different biomaterials and medical devices to block the formation of P. aeruginosa and S. aureus biofilms on those materials [2][3][4][5][6]. Once a biofilm is formed on one of these devices, the only remedy is to replace the infected device, making prevention paramount.…”

Section: Introductionmentioning

confidence: 99%

“…Once a biofilm is formed on one of these devices, the only remedy is to replace the infected device, making prevention paramount. Selenium has additionally shown promise for this purpose in that unlike other antibacterial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating [2][3][4][5][6]. Biofilm formation occurring in a wound itself inhibits wound healing [7,8].…”

Section: Introductionmentioning

confidence: 99%

Organo-Selenium-Containing Polyester Bandage Inhibits Bacterial Biofilm Growth on the Bandage and in the Wound

et al. 2020

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The dressing material of a wound plays a key role since bacteria can live in the bandage and keep re-infecting the wound, thus a bandage is needed that blocks biofilm in the bandage. Using an in vivo wound biofilm model, we examined the effectiveness of an organo-selenium (OS)-coated polyester dressing to inhibit the growth of bacteria in a wound. Staphylococcus aureus (as well as MRSA, Methicillin resistant Staph aureus), Stenotrophomonas maltophilia, Enterococcus faecalis, Staphylococcus epidermidis, and Pseudomonas aeruginosa were chosen for the wound infection study. All the bacteria were enumerated in the wound dressing and in the wound tissue under the dressing. Using colony-forming unit (CFU) assays, over 7 logs of inhibition (100%) was found for all the bacterial strains on the material of the OS-coated wound dressing and in the tissue under that dressing. Confocal laser scanning microscopy along with IVIS spectrum in vivo imaging confirmed the CFU results. Thus, the dressing acts as a reservoir for a biofilm, which causes wound infection. The same results were obtained after soaking the dressing in PBS at 37 °C for three months before use. These results suggest that an OS coating on polyester dressing is both effective and durable in blocking wound infection.

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“…It has been shown that organo-selenium can be covalently attached to many types of surfaces and still generate superoxide radicals (O 2 • ¯) as it has been shown in the past with different types of materials [ 17 , 18 , 19 , 20 , 21 ]. The di-selenide compounds, used in the synthesis of the coating, are reduced by thiols (sulfhydryl groups, R-SH) which can be found in physiological fluids, and form selenide anions, R-Se − [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we showed that organo-selenium compounds could be covalently bound to solid matrixes, and they retained their ability to catalyze the formation of superoxide radicals to inhibit bacterial attachment to a surface [ 17 , 18 , 19 , 20 , 21 ]. The present study looks at the ability of an organo-selenium bandage to not only block the ability of both P. aeruginosa and S. aureus to form a biofilm on the bandage material, but also to inhibit biofilm formation in the underlying wound.…”

Section: Introductionmentioning

confidence: 99%

A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens

et al. 2014

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Biofilm formation in wounds is a serious problem which inhibits proper wound healing. One possible contributor to biofilm formation in a wound is the bacteria growing within the overlying bandage. To test this mechanism, we used bandages that contained a coating of organo-selenium that was covalently attached to the bandage. We tested the ability of this coating to kill bacteria on the bandage and in the underlying tissue. The bandage material was tested with both lab strains and clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis. It was found that the organo-selenium coated bandage showed inhibition, of biofilm formation on the bandage in vitro (7–8 logs), with all the different bacteria tested, at selenium concentrations in the coating of less than 1.0%. These coatings were found to remain stable for over one month in aqueous solution, 15 min in boiling water, and over 6 years at room temperature. The bandages were also tested on a mouse wound model where the bacteria were injected between the bandage and the wound. Not only did the selenium bandage inhibit biofilm formation in the bandage, but it also inhibited biofilm formation in the wound tissue. Since selenium does not leave the bandage, this would appear to support the idea that a major player in wound biofilm formation is bacteria which grows in the overlying bandage.

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Inhibition of Otopathogenic Biofilms by Organoselenium-Coated Tympanostomy Tubes

Abstract: The OSe coating is a potential long-lasting agent to prevent biofilm development on tympanostomy tubes by otopathogens.

Cited by 13 publications

References 25 publications

Next-Science: A Novel Antimicrobial Agent that Inhibits Biofilm Development by Escherichia coli Clinical Isolates on Urinary Tract Catheters

Next-Science: A Novel Antimicrobial Agent that Inhibits Biofilm Development by Escherichia coli Clinical Isolates on Urinary Tract Catheters

Organo-Selenium-Containing Polyester Bandage Inhibits Bacterial Biofilm Growth on the Bandage and in the Wound

A Novel Organo-Selenium Bandage that Inhibits Biofilm Development in a Wound by Gram-Positive and Gram-Negative Wound Pathogens

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