Inhibition of ossification in vivo and differentiation of osteoblasts in vitro by tributyltin

Tsukamoto, Yu; Ishihara, Yoko; Miyagawa‐Tomita, Sachiko; Hagiwara, Hiromi

doi:10.1016/j.bcp.2004.04.020

Cited by 42 publications

(31 citation statements)

References 32 publications

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“…Cultured ROB cells spontaneously differentiate into osteoblasts, and this process is coupled to the increased expression of both collagen and non-collagenous bone proteins, including osteonectin, osteocalcin and osteopontin (9,10,14,(20)(21)(22)(23)(24). Our immunofluorescence findings showed that all cultured ROB cells expressed both osteonectin and collagen-1α, suggesting their osteoblastic lineage.…”

Section: Discussionmentioning

confidence: 55%

Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors

Ziółkowska¹,

Ruciński²,

Tortorella³

et al. 2007

Int J Mol Med

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Abstract. Orexins A and B are hypothalamic peptides which are derived from the proteolytic cleavage of prepro-orexin and act via two subtypes of receptors, named OX1-R (that almost exclusively binds orexin-A) and OX2-R (nonselective for both orexins). Several lines of evidence show that other neuropeptides, which like orexins are involved in the central control of energy homeostasis (e.g. leptin and ghrelin), may play a role in the regulation of bone metabolism, acting via autocrine-paracrine or endocrine routes. Therefore, we studied by reverse transcription-polymerase chain reaction (RT-PCR) the expression of the orexin system in rat calvarial osteoblastlike (ROB) cells, whose osteoblastic lineage was immunocytochemically demonstrated by their osteonectin and collagen-1α content at day 14 of culture. Conventional PCR detected the mRNA expression of OX1-R, but not OX2-R and prepro-orexin in ROB cells at days 2, 7 and 21 of culture. Semiquantitative real time-PCR evidenced a gradual downregulation of OX1-R mRNA in relation to the duration of culture. This novel finding suggests that rat osteoblasts could be a target for circulating orexin-A, especially during their early stages of differentiation into mature osteoblasts.

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Section: Discussionmentioning

confidence: 55%

Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors

Ziółkowska¹,

Ruciński²,

Tortorella³

et al. 2007

Int J Mol Med

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“…In vitro analysis suggested that TBT reduced alkaline phosphatase (ALPase) activity, the rate of calcium deposition, and expression of differentiation markers ALPase and osteocalcin in rat calvarial osteoblast-like cells (ROB cells). The TBT metabolite monobutyltin trichloride (MBT) did not affect fetal skeleton development or osteoblast differentiation of ROB cells (Tsukamoto et al 2004). In mouse embryonic molar development, TBT impaired dentin mineralization and enamel formation, thereby reducing tooth size (Salmela et al 2008).…”

Section: Skeletal Formation and Bone Remodelingmentioning

confidence: 99%

Actions of endocrine-disrupting chemicals on stem/progenitor cells during development and disease

Kopras

Potluri

Bermúdez

et al. 2013

Endocrine-Related Cancer

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Development and fate of the stem cell are regulated by extrinsic signals from the environment. Endocrine-disrupting chemicals which perturb hormonal signaling in utero and during early childhood may cause deregulation of multiple developmental processes, ranging from breakdown of stem cell niche architecture, developmental reprograming and altered stem cell fate to impaired organ and gonad development and sexual differentiation. Therefore, study of the environmental effects on stem cell integrity and normal development is a new and emerging focus for developmental biologists and cell toxicologists. When combined with new human and mouse stem cell-based models, stem cell differentiation dynamics can be studied in more biologically relevant ways. In this study, we review the current status of our understanding of the molecular mechanisms by which endocrine disruptors alter embryonic stem cell and adult stem/progenitor cell fate, organ development, cancer stem cell activity, and tumorigenesis.

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“…TBT has been shown to impair bone mineralization and to have adverse effects on osteoblast differentiation and activity, and calcium deposition in vivo and in vitro [Tsukamoto et al, 2004;Suzuki et al, 2006;Sarpa et al, 2007]. Furthermore, Tsukamoto et al [2004] observed that TBT decreases the expression of genes encoding tissue nonspecific alkaline phosphatase (Alpl) and osteocalcin (Ocn), both associated with mineralization, and inhibits Alpl activity in rat calvarial osteoblast-like cells.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Tsukamoto et al [2004] observed that TBT decreases the expression of genes encoding tissue nonspecific alkaline phosphatase (Alpl) and osteocalcin (Ocn), both associated with mineralization, and inhibits Alpl activity in rat calvarial osteoblast-like cells.…”

Section: Introductionmentioning

confidence: 99%

“…Trisubstituted organotins, tributyltin (TBT) and triphenyltin, have been shown to interfere with biomineralization in different species [Chagot et al, 1990;Adeeko et al, 2003;Tsukamoto et al, 2004;Suzuki et al, 2006;Sarpa et al, 2007;Salmela et al, 2008]. Of the organotins, TBT is estimated to cause the main risk for humans [Risk and Policy Analysts Ltd., 2005].…”

Section: Introductionmentioning

confidence: 99%

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Tributyltin Alters Osteocalcin, Matrix Metalloproteinase 20 and Dentin Sialophosphoprotein Gene Expression in Mineralizing Mouse Embryonic Tooth in vitro

Salmela

Alaluusua²,

Sahlberg³

et al. 2011

Cells Tissues Organs

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We showed in a previous in vitro study that tributyltin (TBT) arrests dentin mineralization and enamel formation in developing mouse tooth. The present aim was to investigate the effect of TBT on the expression of genes associated with mineralization of dental hard tissues. Embryonic day 18 mouse mandibular first molars were cultured for 3, 5 or 7 days and exposed to 1.0 µM TBT and studied by real-time quantitative polymerase chain reaction (RT-QPCR) for the expressions of osteocalcin (Ocn), alkaline phosphatase (Alpl), dentin matrix protein 1 (Dmp1), dentin sialophosphoprotein (Dspp) and matrix metalloproteinase 20 (Mmp-20).Ocn, Mmp-20 and Dspp, whose expressions showed changes in RT- QPCR, were further analyzed by in situ hybridization of tissue sections. In situ hybridization showed that TBT decreased Ocn expression in odontoblasts but increased the expression in the epithelial tooth compartment. In QPCR assays, the net effect in the whole tooth was increased expression. TBT also reduced Mmp-20 expression in ameloblasts and odontoblasts. Dspp expression varied but both QPCR assays and in situ hybridization showed a decreasing trend. TBT exposure had no clear effect on Alpl and Dmp1 expressions. Increased Ocn expression by epithelial enamel organ may inhibit dentin mineralization and enamel formation. Decreased Ocn, Mmp-20 and Dspp expressions in odontoblasts may indicate delayed cell differentiation, or TBT may specifically decrease the expression of genes involved in dentin mineralization. While decreased Mmp-20 expression by TBT in ameloblasts may impair enamel mineralization, the coincident reduction in Mmp-20 and Dspp expressions in odontoblasts may potentiate the delay of dentin mineralization.

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Inhibition of ossification in vivo and differentiation of osteoblasts in vitro by tributyltin

Cited by 42 publications

References 32 publications

Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors

Cultured rat calvarial osteoblast-like cells are provided with orexin type 1 receptors

Actions of endocrine-disrupting chemicals on stem/progenitor cells during development and disease

Tributyltin Alters Osteocalcin, Matrix Metalloproteinase 20 and Dentin Sialophosphoprotein Gene Expression in Mineralizing Mouse Embryonic Tooth in vitro

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