Inhibition of Nitrosative Stress Attenuates Myocardial Injury and Improves Outcomes after Cardiac Arrest and Resuscitation

Wang, Fulin; Yuan, Qiuhuan; Cao, Shengchuan; Li, Ruochuan; Zhang, Jian; Yang, Kehui; Xu, Feng; Chen, Yuguo

doi:10.1097/shk.0000000000001939

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…This potential issue may affect the experimental results. This issue is similar to that of many previous studies on CA (15,38,39), which also relied on human compression of the chest or abdomen. Finally, the hemodynamic mechanisms through which AACD-CPR exerts its effects were not studied.…”

Section: Discussionsupporting

confidence: 79%

Comparison Between Active Abdominal Compression-Decompression Cardiopulmonary Resuscitation and Standard Cardiopulmonary Resuscitation in Asphyctic Cardiac Arrest Rats With Multiple Rib Fractures

Dai,

Zhang,

Wang

et al. 2023

Shock

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Background Active abdominal compression-decompression cardiopulmonary resuscitation (AACD-CPR) is potentially more effective for cardiac arrest with multiple rib fractures. However, its impact on survival rates and neurological outcomes remains unknown. This study aimed to assess if AACD-CPR improves survival rates and neurological outcomes in a rat model of asphyctic cardiac arrest with multiple rib fractures. Methods Adult male Sprague-Dawley rats were randomized into 3 groups: AACD group (n = 15), standard cardiopulmonary resuscitation (STD-CPR) group (n = 15), and sham group (n = 10), after bilateral rib fractures were surgically created and endotracheal intubation was performed. AACD-CPR and STD-CPR groups underwent 8 minutes of asphyxia followed by different CPR techniques. The sham group had venous catheterization only. Physiological variables and arterial blood gases were recorded at baseline and during a 4-hour monitoring period. Neurological deficit scores (NDS) and cumulative survival rates were assessed at 24 h, 48 h, and 72 h. NDS, serum biomarkers and hippocampal neuron analysis were used to evaluate neurological outcomes. Results No statistical differences were observed in the return of spontaneous circulation (ROSC), 24-hour, 48-hour, and 72-hour survival rates between the AACD-CPR and STD-CPR groups. AACD-CPR rats had lower serum levels of neuron-specific enolase (NSE) and S100B at 72 hours post-ROSC, and higher NDS at 72 hours post-ROSC compared to STD-CPR animals. Cellular morphology analysis, H&E staining, and TUNEL/DAPI assays showed more viable neurons and fewer apoptotic neurons in the AACD-CPR group than in the STD-CPR group. Conclusions AACD-CPR can achieve similar survival rates and better neurologic outcome after asphyxial cardiac arrest in rats with multiple rib fractures when compared to STD-CPR.

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Section: Discussionsupporting

confidence: 79%

Comparison Between Active Abdominal Compression-Decompression Cardiopulmonary Resuscitation and Standard Cardiopulmonary Resuscitation in Asphyctic Cardiac Arrest Rats With Multiple Rib Fractures

Dai,

Zhang,

Wang

et al. 2023

Shock

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“…Asphyxia CA/CPR model An asphyxia CA/CPR model was established as previously described with minor modifications (24). After achieving equilibrium for 5 min, CA was induced by intravenous injection of vecuronium (2 mg/kg) with the ventilator turned off.…”

Section: Animal Model Preparationmentioning

confidence: 99%

Sirt3 Mediates the Cardioprotective Effect of Therapeutic Hypothermia After Cardiac Arrest and Resuscitation by Restoring Autophagic Flux via the Pi3k/Akt/Mtor Pathway

Wang,

Xue

et al. 2024

Shock

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Background Postresuscitation cardiac dysfunction is a significant contributor to early death following cardiopulmonary resuscitation (CPR). Therapeutic hypothermia (TH) mitigates myocardial dysfunction due to cardiac arrest (CA); however, the underlying mechanism remains unclear. Sirtuin 3 (Sirt3) was found to affect autophagic activity in recent research, motivating us to investigate its role in the cardioprotective effects of TH in the treatment of CA. Methods Sprague–Dawley rats were used to establish an in vivo CA/CPR model and treated with a selective Sirt3 inhibitor or vehicle. Survival rate, myocardial function, autophagic flux, and Sirt3 expression and activity were evaluated. H9C2 cells were subjected to oxygen–glucose deprivation/reoxygenation (OGD/R) injury in vitro. The cells were transfected with Sirt3-siRNA and treated with the autophagy inhibitor chloroquine or the PI3K inhibitor LY294002, and cell viability and autophagic flux were assessed. Results Rats exhibited decreased survival and impaired cardiac function after CA/CPR, which were alleviated by TH. Mechanistically, TH restored Sirt3 expression and autophagic flux which were impaired by CA/CPR. Sirt3 inactivation diminished the capacity of TH to restore autophagic flux and partially abolished the improvements in myocardial function and survival. An in vitro study further showed that TH-induced restoration of disrupted autophagic flux by OGD/R was attenuated by pretreatment with Sirt3-siRNA, and this attenuation was partially rescued by the inhibition of PI3K/Akt/mTOR signaling cascades. Conclusions Sirt3 mediates the cardioprotective effect of TH by restoring autophagic flux via the PI3K/Akt/mTOR pathway. These findings suggest the potential of Sirt3 as a therapeutic target for CA.

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Early and Accurate Detection of Radiation-induced Heart Damage by Cardiodynamicsgram

Tao

Sun

et al. 2023

J. of Cardiovasc. Trans. Res.

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Inhibition of Nitrosative Stress Attenuates Myocardial Injury and Improves Outcomes after Cardiac Arrest and Resuscitation

Cited by 3 publications

References 34 publications

Comparison Between Active Abdominal Compression-Decompression Cardiopulmonary Resuscitation and Standard Cardiopulmonary Resuscitation in Asphyctic Cardiac Arrest Rats With Multiple Rib Fractures

Comparison Between Active Abdominal Compression-Decompression Cardiopulmonary Resuscitation and Standard Cardiopulmonary Resuscitation in Asphyctic Cardiac Arrest Rats With Multiple Rib Fractures

Sirt3 Mediates the Cardioprotective Effect of Therapeutic Hypothermia After Cardiac Arrest and Resuscitation by Restoring Autophagic Flux via the Pi3k/Akt/Mtor Pathway

Early and Accurate Detection of Radiation-induced Heart Damage by Cardiodynamicsgram

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