Inhibition of nitrite formation from hydroxylammoniumchloride: A simple assay for superoxide dismutase

Elstner, Erich F.; Heupel, Adelheid

doi:10.1016/0003-2697(76)90488-7

Cited by 1,572 publications

(626 citation statements)

References 12 publications

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“…Superoxide production was determined by adding hydroxylamine (0.5 mM; Sigma-Aldrich) (21,22) to cocultures of infected macrophages and live neutrophils. Hydroxilamine converts superoxide into nitrite, which can then be quantitated by performing the Griess reaction, as described above.…”

Section: No and Superoxide Detectionmentioning

confidence: 99%

Neutrophils and Macrophages Cooperate in Host Resistance againstLeishmania braziliensisInfection

Novais

Santiago²,

Báfica³

et al. 2009

The Journal of Immunology

132

121

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Neutrophils play an active role in the control of infections caused by intracellular pathogens such as Leishmania. In the present study, we investigated the effect of neutrophil depletion at the time of Leishmania braziliensis infection of BALB/c mice and how neutrophils interact with the infected macrophage to promote parasite elimination. The in vivo depletion of neutrophils led to a significant increase in parasite load and enhanced the Th1-Th2 immune response in this experimental model of infection. BALB/c mice coinoculated with both parasites and live neutrophils displayed lower parasite burdens at the site of infection and in the draining lymph nodes. In vitro, we observed that live neutrophils significantly reduced the parasite load in L. braziliensis-infected murine macrophages, an effect not observed with Leishmania major. L. braziliensis elimination was dependent on the interaction between neutrophils and macrophages and was associated with TNF-α as well as superoxide production. Furthermore, cooperation between neutrophils and macrophages toward parasite elimination was also observed in experiments performed with L. braziliensis-infected human cells and, importantly, with two other New World Leishmania species. These results indicate that neutrophils play an important and previously unappreciated role in L. braziliensis infection, favoring the induction of a protective immune response.

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Section: No and Superoxide Detectionmentioning

confidence: 99%

Neutrophils and Macrophages Cooperate in Host Resistance againstLeishmania braziliensisInfection

Novais

Santiago²,

Báfica³

et al. 2009

The Journal of Immunology

132

121

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“…Briefly, inhibition of nitrite formation from hydroxyl-ammonium chloride was determined by a spectrophotometric method (adapted from Elstner and Heupel (19)). Experiments were performed in triplicate.…”

Section: Enzymatic Assays Of Sod-1 Activitymentioning

confidence: 99%

Cu/Zn Superoxide Dismutase Plays Important Role in Immune Response

Marikovsky

Ziv

Nevo³

et al. 2003

The Journal of Immunology

183

112

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Activation of macrophages leads to the secretion of cytokines and enzymes that shape the inflammatory response and increase metabolic processes. This, in turn, results in increased production of reactive oxygen species. The role of Cu/Zn superoxide dismutase (SOD-1), an important enzyme in cellular oxygen metabolism, was examined in activated peritoneal elicited macrophages (PEM) and in several inflammatory processes in vivo. LPS and TNF-α induced SOD-1 in PEM. SOD-1 induction by LPS was mainly via extracellular signal-regulated kinase-1 activation. Transgenic mice overexpressing SOD-1 demonstrated a significant increase in the release of TNF-α and of the metalloproteinases MMP-2 and MMP-9 from PEM. Disulfiram (DSF), an inhibitor of SOD-1, strongly inhibited the release of TNF-α, vascular endothelial growth factor, and MMP-2 and MMP-9 from cultured activated PEM. These effects were prevented by addition of antioxidants, further indicating involvement of reactive oxygen species. In vivo, transgenic mice overexpressing SOD-1 demonstrated a 4-fold increase in serum TNF-α levels and 2-fold stronger delayed-type hypersensitivity reaction as compared with control nontransgenic mice. Conversely, oral administration of DSF lowered TNF-α serum level by 4-fold, lowered the delayed-type hypersensitivity response in a dose-dependent manner, and significantly inhibited adjuvant arthritis in Lewis rats. The data suggest an important role for SOD-1 in inflammation, establish DSF as a potential inhibitor of inflammation, and raise the possibility that regulation of SOD-1 activity may be important in the treatment of immune-dependent pathologies.

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“…For determination of superoxide dismutase activity, the Triton-X 1 00-treated homogenate was sonicated ac cording to the method of Stein et al (20). Superoxide dismutase activity was deter mined by the method of Elstner and Heupei (21), and it was calculated using logit paper (22). Catalase activity with hydrogen peroxide as the substrate was determined by the meth od of Decker (23), and the activity was re presented in international units.…”

Section: Methodsmentioning

confidence: 99%

Changes in lipid peroxidation and activities of xanthine oxidase, superoxide dismutase and catalase in kidneys of cephaloridine-administered rats.

Suzuki

Sudo

1989

Jpn.J.Pharmacol

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Inhibition of nitrite formation from hydroxylammoniumchloride: A simple assay for superoxide dismutase

Cited by 1,572 publications

References 12 publications

Neutrophils and Macrophages Cooperate in Host Resistance againstLeishmania braziliensisInfection

Neutrophils and Macrophages Cooperate in Host Resistance againstLeishmania braziliensisInfection

Cu/Zn Superoxide Dismutase Plays Important Role in Immune Response

Changes in lipid peroxidation and activities of xanthine oxidase, superoxide dismutase and catalase in kidneys of cephaloridine-administered rats.

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