Inhibition of nitrification and carbon dioxide evolution as rapid tools for ecotoxicological assessment of drilling fluids

Odokuma, Lucky O.; Akponah, E.

doi:10.4314/ajazeb.v6i1.41168

Cited by 7 publications

(7 citation statements)

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“…Samples were collected in sterile plastic bottles (Tarson). Column water samples were collected for the analysis of THB, whereas bottom water samples were collected for the analysis of Nitrosomonas and Nitrobacter (Odokuma & Akponah 2008). THB was enumerated using the spread plate method (Cappuccino & Sherman 1992); 0.1 ml of the sample was spread in the plates and incubated in an inverted position at 37°C for 20-24 h. Simultaneously collected samples were processed on Winogradskyi medium for the isolation of nitrifying bacteria.…”

Section: Bacteriological Analysesmentioning

confidence: 99%

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Saha

Sarkar

Bandyopadhyay

2021

Water Science and Technology

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East Kolkata Wetlands (EKW) is designated as International Ramsar site and are the hotspot for large-scale wastewater aquaculture practices. However, the continued surveillance of physicochemical properties of water and application of an eco-friendly approach are essential to ensure safe aquaculture practices. In the present study, we assessed the seasonal variation in physicochemical parameters of water across EKW and investigated the role of nitrifying bacteria as probiotics. We statistically analyzed various physicochemical properties of water samples from EKW. Results of the statistical analysis indicated a significant variation in all the physicochemical parameters across the selected water bodies of EKW (p < 0.01). We isolated and enumerated Nitrosomonas sp. and Nitrobacter sp. and assessed their ability to degrade trichloroethylene (TCE). The role of Nitrosomonas and Nitrobacter sp. were further investigated and established through a small-scale experiment. Two microbial isolates, NSW3 and NBW2, displayed superior TCE degradation ability at pH 5, and the application of these strains as probiotics were found to improve the quality of water and survival rate of fishes in the treated experimental tanks. Our findings suggest that the application of the above mixed bacterial cultures in aquaculture could be an effective and environment-friendly approach for safe and productive aquaculture operations.

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Section: Bacteriological Analysesmentioning

confidence: 99%

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Saha

Sarkar

Bandyopadhyay

2021

Water Science and Technology

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“…The low hydrocarbon content along with other chemical compositions of drilling muds are responsible for their toxicity and also the fact that synthetic based mud does not disperse in water is an additional contributing factor to its toxicity and low biodegradation. Several authors had shown that the toxicity of drilling muds may be linked to their chemical entities such as the base fluid types (Odokuma and Okpokwasili 1992;Ekpo and Ekanem, 2000;Odokuma and Ikpe 2003), concentration, water solubility (Odokuma and Akponah, 2008) and genetic constitution of the organism (Dutton et al, 1990). Therefore, oil exploration and production companies operating in Ologbo community in Edo State, should be encouraged to put into consideration the effect of…”

Section: Discussionmentioning

confidence: 99%

“…Research have abundantly shown that drilling muds additives may contain toxic substances such as heavy metals, hydrocarbons, biocides, chromate, organic polymers and trace elements that have the tendency to bioaccumulate and interfere with normal biological activities of organisms (Odokuma and Ikpe, 2003;Odokuma and Akponah, 2008;Vincent-Akpu et al, 2010). Several studies according to Engelhard et al, (1989) and Vincent-Akpu et al, (2010), have been conducted with various drilling fluids in the North Sea using mortality as the criterion for determining their effects on the biota.…”

Section: Introductionmentioning

confidence: 99%

The effect of composition on the biodegradability and toxicity of drilling muds used at ologbo active onshore field, Edo State, Nigeria

2013

Int. J. Biosci.

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The effect of composition on the biodegradability and toxicity of two muds commonly used at onshore oil fields at Ologbo, Edo State were examined. Biodegradation of drill muds by two bacterial and fungal isolates; Enterobacter aerogenes, Micrococcus sp., Aspergillus sp. and Penicillium sp. were carried in a shake flask experiment using mineral salts medium at 120 rpm for 28 days. The total viable counts were monitored and ultimate biodegradability was derived from the ratio of COD and BOD5, after every four days. Lethal effects of the drilling muds on juvenile Tilapia guineensis, Micrococcus sp. and Penicillium sp. were investigated using static renewal bioassay for 96 hr and 24 hr. The Potassium chloride (KCl) polymer water based mud (WBM) was more biodegradable than synthetic based mud (SBM). This was indicated by the highest total viable counts recorded in consortium amended with water based mud (101 ×10 3 cfu/ml), and also recording the lowest chemical oxygen demand and biological oxygen demand (47 mg/l and 0.4 mg/l respectively).There were no significant differences (P > 0.05) in the degradation of the muds by the isolates. The 96 hr LC50 of potassium chloride (KCl) polymer water based mud and synthetic based mud (SBM) were 8125 mg/l and 5800 mg/l for Tilapia guineensis respectively. The 24 hr LC50 of Potassium Chloride polymer water based mud was 200 mg/l for Micrococcus sp. and Penicillium sp. Exploration and production companies operating in Ologbo should be encouraged to put into consideration the effect composition of the drilling muds before usage.

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“…The harmful effect of chemicals has on organism depend on many different factors such as the type of organism; age; size; population; concentration of the toxicant; etc., [6]. Microorganisms found in fresh water; brackish water and marine water such as bacteria; fungal; viruses and protozoa; can influence the tri-aquatic ecosystem ability to sustain life on earth [7]. Bacteria such as Nitrobacter are also present in the fresh water; brackish water and marine water [3].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of bitumen on soil fertility bacteria - Nitrobacter and Nitrosomonas from Tekulu Waterside, Rivers State, Nigeria

Chidi Juliet Nwankwo,

Lucky Obukowho Odokuma,

Chimezie Jason Ogugbue

2023

GSC Adv. Res. Rev.

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Toxicity of bitumen on Nitrosomonas and Nitrobacter from Tekulu Waterside, Bodo in Gokana Local government area of Ogoni, Rivers State, Nigeria was carried out using standard analytical procedures. The density, API and flash point of the bitumen used in this study at 15°C was 0.9898 g/cm3, API at 60°F value of 11.46 and >200°C respectively. The total TPH and PAH constituent of the bitumen was 20,549.77 mg/L and 924.09 mg/L respectively. Analysis of the effect of different concentration (50 mg/L, 250 mg/L, 500 mg/L, 750 mg/L and 1000 mg/L) of bitumen on Nitrosomonas sp. over a period (24hrs, 48hrs. 72hrs and 96hrs) revealed that the mortality of the organism increased as the concentration of the toxicant. The mortality was also affected by the duration of exposure. The probit analysis on the lethal concentration (LC10, LC20 and LC50) of the bitumen on Nitrosomonas sp. was 223.32 mg/L, 369.682 mg/L and 969.622 mg/L respectively and Nitrobacter sp. 587.837 mg/L, 765.74 mg/L and 1269.841 mg/L respectively, when exposed for 96 hours revealed a varying mortality status. The LC50 of the bitumen on Nitrosomonas sp. peaked at 48hrs. The study shows that bitumen was more toxic to Nitrosomonas than Nitrobacter sp. based on the findings of the study, it is recommended that test organisms (Nitrosomonas sp. and Nitrobacter sp.) could serve as a potential tool for eco-toxicological assay and pollution biomarker.

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Inhibition of nitrification and carbon dioxide evolution as rapid tools for ecotoxicological assessment of drilling fluids

Cited by 7 publications

References 0 publications

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

Water quality assessment of East Kolkata Wetland with a special focus on bioremediation by nitrifying bacteria

The effect of composition on the biodegradability and toxicity of drilling muds used at ologbo active onshore field, Edo State, Nigeria

Toxicity of bitumen on soil fertility bacteria - Nitrobacter and Nitrosomonas from Tekulu Waterside, Rivers State, Nigeria

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