Inhibition of nitric oxide production on LPS-activated macrophages by kazinol B from Broussonetia kazinoki

Ryu, Jae‐Ha; Ahn, Hanna; Lee, Hwa Jin

doi:10.1016/s0367-326x(03)00062-5

Cited by 41 publications

(28 citation statements)

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“…First, massive amounts of NO generated by activated macrophages may be responsible not only for immunological defense against tumor cells and pathogens, but also may cause injury in cells, damaging several tissues. In addition, the excess of NO and TNF-␣ is implicated in sepsis and endotoxic shock during an inflammatory process (Chi et al, 2003;Ryu et al, 2003b). Researchers have attempted to verify the immunomodulatory properties of plant extracts in order to avoid these undesirable consequences (Rimbach et al, 2000;Ferreira et al, 2003;Ryu et al, 2003a,b).…”

Section: Discussionmentioning

confidence: 96%

“…Among other properties of Casearia sylvestris we can cite the cytotoxic activity in tumor cells (Bolzani et al, 1999;Orberlies et al, 2002). Immunomodulatory properties of plant extracts have been extensively described, especially for their inhibitory activity on nitric oxide (NO) production by macrophages (Rimbach et al, 2000;Ryu et al, 2003a;Ferreira et al, 2003;Ryu et al, 2003b). Immunological activity against pathogens in hosts includes the participation of macrophages that eliminate foreign agents by phagocytosis and generate reactive nitrogen products such as nitric oxide (Reis et al, 2001).…”

Section: Introductionmentioning

confidence: 97%

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Down-modulation of nitric oxide production in murine macrophages treated with crude plant extracts from the Brazilian Cerrado

Napolitano

Mineo

Souza

et al. 2005

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Down-modulation of nitric oxide production in murine macrophages treated with crude plant extracts from the Brazilian Cerrado

Napolitano

Mineo

Souza

et al. 2005

Journal of Ethnopharmacology

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“…There are several bioactive structures with gem-dimethylpyran that showed cytotoxicity against cancer cells 18) or inhibitory activity of NO production in activated murine macrophages. 29) The difference in the skeleton between flavone and chalcone did not show a big difference in activity potency. A meroterpenoid, bakuchiol from Psoralea corylifolia was reported as an inhibitor of iNOS in LPS-activated murine macrophages.…”

Section: Discussionmentioning

confidence: 99%

Prenylflavones from Psoralea corylifolia Inhibit Nitric Oxide Synthase Expression through the Inhibition of I-.KAPPA.B-.ALPHA. Degradation in Activated Microglial Cells

Lee

Kim

Ryu

2005

Biological & Pharmaceutical Bulletin

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Microglia, the immune regulatory and inflammatory cells of the central nervous system (CNS), can be activated in response to a variety of neurodegenerative and neuroinflammatory conditions. Activation of microglia results in the production of proinflammatory materials such as tumor necrosis factor-a (TNF-a), interleukin-1 and 6, reactive oxygen species (ROS), and nitric oxide (NO) in response to injury. [1][2][3][4] Large amounts of NO produced by inducible nitric oxide synthase (iNOS) can rapidly react with superoxide anion radical (· O 2 Ϫ ) to produce peroxynitrite anion (ONOO Ϫ ). 5,6) These reactive nitrogen species might be involved in the pathogenesis of various neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease and ischemia. 7) As a neuromodulator in CNS, NO participates in brain development, pain perception, memory, neuronal plasticity and behavior.8) The excessive amounts of NO has been observed in damaged tissues associated with type I diabetes, arthritis, or nephritis, 9) suggesting that deregulation of iNOS expression is linked to many diseases. The expression and activity of iNOS are increased in chronic diseases, such as inflammation or cancer.10) Generally the iNOS along with the release of NO by microglia contributes to progress neurodegeneration and aggravate diseases. Therefore, the inhibitors of NO production may have beneficial therapeutic effects in the treatment of diverse diseases.In order to find new iNOS inhibitors from medicinal plants, we have screened inhibitory activity of NO production in lipopolysaccharide (LPS)-activated BV-2 mouse microglial cells. The methanol extract of Psoralea corylifolia L. (Fabaceae) showed remarkable inhibitory effects on NO production. The seeds of P. corylifolia have been used traditionally for the treatment of stomachic, deobstruent, anthelmintic, diuretic, vitiligo and certain skin diseases. 11,12) Several reports revealed that its seeds contain antimutagenic coumarins, antioxidants, antibacterial and antiplatelet flavonoids. [13][14][15][16] This article describes the identification of two prenylflavonoids from Psoralea corylifolia and their inhibitory activity of NO production in LPS-activated microglial cells. MATERIALS AND METHODS Plant MaterialsThe dried seeds of Psoralea corylifolia were purchased from the Kyungdong oriental drug market in Seoul, Korea and authenticated by Prof. T. H. Kim at the College of Pharmacy, Sookmyung Women's University. A voucher specimen (No. SPH 04005) was deposited in the herbarium of the Sookmyung Women's University. The plant materials (540 g) were extracted three times with MeOH and the combined extracts (130 g) were successively partitioned with n-hexane, EtOAc and BuOH.The EtOAc soluble fraction (53 g) was subjected to silica gel column chromatography eluting with an n-hexane-EtOAc gradient system (50 : 1-1 : 5) to afford active fractions A 1 (0.8 g) and A 2 (3.6 g). Repeated silica gel column chromatography of fraction A 1 with a gradient elution of n-hexaneEtOAc (30 : 1-1 : 1) to give ...

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“…To determine inhibition of NO formation, cells were stimulated and treated with test compounds as above. NO levels were determined by measuring nitrite production spectrophotometrically using the Greiss reagent (Ryu et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Peracetylation as a Means of Enhancing in Vitro Bioactivity and Bioavailability of Epigallocatechin-3-Gallate

et al. 2006

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ABSTRACT:(؊)-Epigallocatechin-3-gallate (EGCG) is the widely studied catechin in green tea (Camellia sinensis). Previously, we have reported the low bioavailability of EGCG in rats and mice. As a means of improving the bioavailability of EGCG, we have prepared a peracetylated EGCG derivative (AcEGCG) and herein report its growth inhibitory activity and cellular uptake in vitro, as well as bioavailability in mice. AcEGCG exhibited enhanced growth inhibitory activity relative to EGCG in both KYSE150 human esophageal (IC 50

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Inhibition of nitric oxide production on LPS-activated macrophages by kazinol B from Broussonetia kazinoki

Cited by 41 publications

References 14 publications

Down-modulation of nitric oxide production in murine macrophages treated with crude plant extracts from the Brazilian Cerrado

Down-modulation of nitric oxide production in murine macrophages treated with crude plant extracts from the Brazilian Cerrado

Prenylflavones from Psoralea corylifolia Inhibit Nitric Oxide Synthase Expression through the Inhibition of I-.KAPPA.B-.ALPHA. Degradation in Activated Microglial Cells

Peracetylation as a Means of Enhancing in Vitro Bioactivity and Bioavailability of Epigallocatechin-3-Gallate

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