Inhibition of Nickel Nanoparticles-Induced Toxicity by Epigallocatechin-3-Gallate in JB6 Cells May Be through Down-Regulation of the MAPK Signaling Pathways

Abstract

With the rapid development in nanotechnology, nickel nanoparticles (Ni NPs) have emerged in the application of nanomedicine in recent years. However, the potential adverse health effects of Ni NPs are unclear. In this study, we examined the inhibition effects of epigallocatechin-3-gallate (EGCG) on the toxicity induced by Ni NPs in mouse epidermal cell line (JB6 cell). MTT assay showed that Ni NPs induced cytotoxicity in a dose-dependent manner while EGCG exerted a certain inhibition on the toxicity. Additionally, EGCG could reduce the apoptotic cell number and the level of reactive oxygen species (ROS) in JB6 cells induced by Ni NPs. Furthermore, we observed that EGCG could down-regulate Ni NPs-induced activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) activation in JB6 cells, which has been shown to play pivotal roles in tumor initiation, promotion and progression. Western blot indicated that EGCG could alleviate the toxicity of Ni NPs through regulating protein changes in MAPK signaling pathways. In summary, our results suggest that careful evaluation on the potential health effects of Ni NPs is necessary before being widely used in the field of nanomedicine. Inhibition of EGCG on Ni NPs-induced cytotoxicity in JB6 cells may be through the MAPK signaling pathways suggesting that EGCG might be useful in preventing the toxicity of Ni NPs.

miR‐26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN‐PI3K/AKT pathway in dairy goats

“…The methyl thiazolyl tetrazolium (MTT) (Sigma, St. Louis, MO) colorimetric assay was used to screen for cell proliferation as previously described (Sun, He, et al, ). To further investigate cell proliferation, a cell cycle staining Kit was used (Liankebio, Hangzhou, China) according to the manufacturer's instructions (Gu et al, ). Cell apoptosis analysis was carried out using the Annexin V‐FITC/PI apoptosis kit (Lager et al, ), Annexin‐V‐positive and PI‐negative cells were defined as early apoptotic cells, and the late apoptotic cells were Annexin‐V and PI positive cells.…”

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

“…Cells were harvested 48 h later and washed three times by cold PBS, and then fixed in 70% ethanol in PBS at -20°C overnight. And then the cells were incubated with 0.5 ml of propidium iodide (PI) staining buffer, which contains 200 μg/ml RNase A and 50 μg/ml PI, at 37°C in the dark for 30 min [ 36 ]. Analyses were performed on BD LSR flow cytometer (BD Biosciences, San Diego, CA).…”

Luteolin inhibits multi-heavy metal mixture-induced HL7702 cell apoptosis through downregulation of ROS-activated mitochondrial pathway

“…Normally, ROS generation and quenching are in a dynamic balance state due to the intracellular antioxidant system. Certain harmful extracellular factors may break this balance, resulting in excessive ROS generation beyond the cell scavenging ability to then induce organelle damage, abnormal expression of proteins or eventually cell death ( 33 – 35 ). Studies have demonstrated that most heavy metal ions cause excessive intracellular ROS generation ( 12 , 22 , 36 , 37 ).…”