Inhibition of MYC in macrophages: tumor vs inflammation-related diseases

Cano-Ramos, Emilio; Lavin, Begoña; Pello, Óscar M.

doi:10.4161/21624011.2014.956013

Cited by 5 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enhanced hyper-methylation of these genes by post-exercise milk product intake may suppress MAP kinase signaling, as well as NF-κB signaling pathways [ 53 – 56 ]. On the other hand, enhanced hypo-methylation of MYC by post-exercise milk product intake ( Fig 6 ) may be a reaction to the aforementioned hyper-methylation to balance the inflammatory response [ 57 , 58 ]. Taken together, these results suggest that exercise training combined with milk product intake suppresses not only NF-κB signaling but also other inflammatory signaling pathways, which is consistent with previous observations indicating that exercise training suppresses multiple transcriptomic networks associated with chronic systemic inflammation [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study

et al. 2017

View full text Add to dashboard Cite

BackgroundMuscle atrophy with aging is closely associated with chronic systemic inflammation and lifestyle-related diseases. In the present study, we assessed whether post-exercise milk product intake during 5-month interval walking training (IWT) enhanced the increase in thigh muscle strength and ameliorated susceptibility to inflammation in older women.MethodsSubjects [n = 37, 66±5 (standard deviation) yrs] who had been performing IWT for >6 months participated in this study. They were randomly divided into the following 3 groups: IWT alone (CNT, n = 12), IWT + low-dose post-exercise milk product intake (LD, n = 12; 4 g protein and 3 g carbohydrate) or IWT + a 3-times higher dose of milk product intake than the LD group (HD, n = 13). They were instructed to repeat ≥5 sets of fast and slow walking for 3 min each at ≥70% and 40% peak aerobic capacity for walking, respectively, per day for ≥4 days/week.ResultsAfter IWT, thigh muscle strength increased in the HD group (8±2%) more than in the CNT group (-2±3%, P = 0.022), despite similar IWT achievements between the groups (P>0.15). Pyrosequencing analysis using whole blood showed that methylation of NFKB1 and NFKB2, master genes of inflammation, was enhanced in the HD group (29±7% and 44±11%, respectively) more than in the CNT group (-20±6% and -10±6%, respectively; P<0.001). Moreover, the genome-wide DNA methylation analysis showed that several inflammation-related genes were hyper-methylated in the HD group compared with that in the CNT group, suggesting greater pro-inflammatory cytokine gene suppression in the HD group.ConclusionHD milk product intake after exercise produced a greater percent increase in thigh muscle strength and NFKB1 and NFKB2 gene methylation during IWT in physically active older women.Trial registrationUMIN-CTR No. UMIN000024544 and No. UMIN000024912

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The Myc gene is well known as a proto-oncogene that is over expressed in various types of cancers include lymphomas, lung carcinoma, breast carcinoma and colon carcinomas [80]. Myc is also known to be involved in the polarization of M2 tumor-associated macrophages and is targeted for new therapeutic strategies against cancer [81][82][83][84].…”

Section: Transcriptomic Studymentioning

confidence: 99%

Toxicity of TiO2 Nanoparticles: Validation of Alternative Models

Leroux

Doumandji

Chézeau

et al. 2020

IJMS

View full text Add to dashboard Cite

There are many studies concerning titanium dioxide (TiO2) nanoparticles (NP) toxicity. Nevertheless, there are few publications comparing in vitro and in vivo exposure, and even less comparing air–liquid interface exposure (ALI) with other in vitro and in vivo exposures. The identification and validation of common markers under different exposure conditions are relevant for the development of smart and quick nanotoxicity tests. In this work, cell viability was assessed in vitro by WST-1 and LDH assays after the exposure of NR8383 cells to TiO2 NP sample. To evaluate in vitro gene expression profile, NR8383 cells were exposed to TiO2 NP during 4 h at 3 cm2 of TiO2 NP/cm2 of cells or 19 μg/mL, in two settings—submerged cultures and ALI. For the in vivo study, Fischer 344 rats were exposed by inhalation to a nanostructured aerosol at a concentration of 10 mg/m3, 6 h/day, 5 days/week for 4 weeks. This was followed immediately by gene expression analysis. The results showed a low cytotoxic potential of TiO2 NP on NR8383 cells. Despite the absence of toxicity at the doses studied, the different exposures to TiO2 NP induce 18 common differentially expressed genes (DEG) which are involved in mitosis regulation, cell proliferation and apoptosis and inflammation transport of membrane proteins. Among these genes, we noticed the upregulation of Ccl4, Osm, Ccl7 and Bcl3 genes which could be suggested as early response biomarkers after exposure to TiO2 NP. On the other hand, the comparison of the three models helped us to validate the alternative ones, namely submerged and ALI approaches.

show abstract

“…These results suggest that M1 show a compromised fitness compared to M2. In addition M2 cells upregulated c-Myc expression as compared to M1 ( Pello, 2016 ; Pello et al, 2012a , b ; Cano-Ramos et al, 2014 ) ( Fig. 1 P).…”

Section: Resultsmentioning

confidence: 85%

Classically-activated macrophages elimination in tumor-conditioned medium by alternatively-activated macrophages

2017

View full text Add to dashboard Cite

Cellular interactions are critical during development, tissue fitness and epithelial tumor development. The expression levels of specific genes confer to tumoral cells a survival advantage versus the normal neighboring cells. As a consequence, cells surrounding tumors are eliminated and engulfed by macrophages. We propose a novel scenario in which circulating cells facing a tumor can reproduce these cellular interactions. In vitro cultured macrophages from murine bone marrow were used to investigate this hypothesis. M1 macrophages in tumoral medium upregulated markers of a suboptimal condition, such as Sparc and TyrRS, and undergo apoptosis. However, M2 macrophages display higher Myc expression levels and proliferate at the expense of M1. Resulting M1 apoptotic debris is engulfed by M2 in a Sparc- and TyrRS-dependent manner. These findings suggest that tumor-dependent macrophage elimination could deplete immune response against tumors. This possibility could be relevant for macrophage based anti-tumoral strategies.

show abstract

Inhibition of MYC in macrophages: tumor vs inflammation-related diseases

Cited by 5 publications

References 10 publications

Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study

Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study

Toxicity of TiO2 Nanoparticles: Validation of Alternative Models

Classically-activated macrophages elimination in tumor-conditioned medium by alternatively-activated macrophages

Contact Info

Product

Resources

About