Inhibition of mitochondrial gene transcription suppresses neurotensin secretion in the human carcinoid cell line bon

Li, Nan; Wang, QingDing; Wang, Xiaofu; Hellmich, Mark R.; Greeley, George H.; Evers, B. Mark

doi:10.1016/s0016-5085(00)83291-6

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Association Of Myosin Va With Secretory Granules1

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2007

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“…These enterochromaffin cells store various peptides in SGs and release them in response to external stimuli (Tran et al, 2004;Li et al, 2005;Huet et al, 2006). BON cells express Rab27a, Myrip (data not shown), and MyoVa ( Fig.…”

Section: Association Of Myosin Va With Secretory Granulesmentioning

confidence: 92%

Myosin Va Mediates Docking of Secretory Granules at the Plasma Membrane

Desnos¹,

Huet²,

Fanget³

et al. 2007

J. Neurosci.

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Ϫ4 m 2 ⅐ s Ϫ1 ) were considered as docked at the plasma membrane based on two properties: (1) SGs that undergo exocytosis have a D xy below this threshold value for at least 2 s before fusion; (2) a negative autocorrelation of the vertical motion was found in subtrajectories with a D xy below the threshold. Using this criterion of docking, we found that the main effect of MyoVa inhibition was to reduce the number of docked granules, leading to reduced secretory responses. Surprisingly, this reduction was not attributable to a decreased transport of SGs toward release sites. In contrast, MyoVa silencing reduced the occurrence of long-lasting, but not short-lasting, docking periods. We thus propose that, despite its known motor activity, MyoVa directly mediates stable attachment of SGs at the plasma membrane.

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Section: Association Of Myosin Va With Secretory Granulesmentioning

confidence: 92%