Inhibition of miR146b‐5p suppresses CT‐guided renal cell carcinoma by targeting TRAF6

Meng, Gaopei; Li, Guoce; Yang, Xue; Xiao, Na

doi:10.1002/jcb.27566

Cited by 11 publications

(12 citation statements)

References 30 publications

(69 reference statements)

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“…In NSCLC, ectopic expression of miR-146b-5p inhibits cancer cell proliferation and induces cell cycle arrest, while the expression of miR-146b-5p is negatively correlated with the expression of TRAF6 (56). However, in renal cell carcinoma, blocking miR-146b-5p inhibits tumor growth and potentiates the inflammatory response by increasing the expression of TRAF6 (21). These different results illustrate the complexity of cancer management and confirm the importance of clarifying the functional role of the miR-146b-5p-TRAF6 axis in CRC.…”

Section: Discussionmentioning

confidence: 99%

“…CRC cells by targeting pyruvate dehydrogenase E1 subunit β (PDHB) (19); however, its role in CRC remains unknown. As a downstream target of miR-146b-5p, tumor necrosis factor receptor-associated factor 6 (TRAF6) has been reported to be a prognostic biomarker in several types of cancer, such as glioma (20), renal cell carcinoma (21), osteosarcoma (22) and hepatocellular carcinoma (23). However, the mechanism by which TRAF6 may function downstream of miR-146b-5p in CRC remains unclear.…”

Section: Mir-146b-5p Promotes Colorectal Cancer Progression By Target...mentioning

confidence: 99%

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miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6

Shi

Zheng

et al. 2022

Exp Ther Med

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Increasing evidence highlights the multiple roles of microRNAs (miRs) in the tumorigenesis of colorectal cancer (CRC); however, the molecular mechanism, particularly the target of miR-146b-5p in CRC has not been fully elucidated. The present study aimed to elucidate the influence of miR-146b-5p via regulating tumor necrosis factor receptor-associated factor 6 (TRAF6) in CRC. The expression levels of miR-146b-5p and TRAF6 in CRC tissue and cells were determined by reverse transcription quantitative PCR and western blotting. Binding between miR-146b-5p and TRAF6 was examined using a dual luciferase reporter gene assay. The impact of miR-146b-5p and TRAF6 on proliferation and migration of CRC cells was determined using Cell Counting Kit-8 and Transwell assays, respectively. An animal model of CRC was established to determine the carcinogenic effect of the miR-146b-5p-TRAF6 axis. The results demonstrated that miR-146b-5p was highly expressed in CRC tissue samples compared with in normal adjacent tissue samples and in CRC cells compared with in the normal NCM460 cell line, whereas TRAF6 was expressed at low levels. Overexpression of miR-146b-5p decreased TRAF6 expression in CRC HT29 and SW620 cells. miR-146b-5p targeted and inhibited TRAF6 expression in CRC cells. Furthermore, transfection with a miR-146b-5p mimic promoted the proliferation, migration and invasion of CRC cells and tumor growth; however, these effects were abolished by TRAF6 overexpression. Transfection with a miR-146b-5p inhibitor suppressed the proliferation of CRC cells. Taken together, the results from the present study demonstrated that miR-146b-5p could enhance the initiation and tumorigenesis of CRC by targeting TRAF6. These results will help elucidate the mechanisms underlying CRC development and will facilitate the development of targeted therapy for CRC.

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Section: Discussionmentioning

confidence: 99%

Section: Mir-146b-5p Promotes Colorectal Cancer Progression By Target...mentioning

confidence: 99%

miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6

Shi

Zheng

et al. 2022

Exp Ther Med

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“…MiR-146b-5p has important and complex functions and its dysregulation been discovered in a variety of human malignancies. It is overexpressed in some malignant tumors, such as colorectal cancer [23], thyroid cancer [24], renal cell carcinoma [25], and osteosarcoma [26], and acts as an onco-miRNA, promoting cancer growth and metastasis. In other cancers, such as non-small cell lung cancer [27], gallbladder carcinoma [28], and glioma [29], miR-146b-5p functions as a tumor suppressor, inhibiting cancer development.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Network Analysis Reveals Prognosis-Related CircRNAs in Pancreatic Cancer Cells

Zhang

Zeng

et al. 2020

Preprint

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Objective: To construct a key prognosis-related regulatory network and to identify the epigenomic alterations of RNAs that have crucial functions in cancer pathogenesis.Methods: RNA expression profiles of miRNAs, mRNAs and circRNAs were extracted from the NCBI GEO and EBI ArrayExpress databases. The identification of differentially expressed genes was performed by R language. Databases such as starBase and TCGA were used for annotation, visualization, and integrated discovery. GO and KEGG pathway enrichment analyses were performed by DAVID 6.8. The key prognosis-related regulatory network was constructed with Cytoscape software. The involved circRNAs were verified by quantitative real-time PCR (qRT-PCR) in five pancreatic cancer cell lines. Proliferation ability was assessed by the CCK-8 assay, apoptosis was detected by flow cytometry, and migratory and invasive abilities were assessed by Transwell assays.Results: In total, 798 differentially expressed genes, consisting of 85 circRNAs, 19 miRNAs and 694 mRNAs, were obtained. A miRNA interaction network was predicted and used to construct a prognosis-related regulatory network. GO annotation and KEGG pathway analysis revealed important biological processes and pathways in pancreatic cancers. The key regulatory factors in the network were miR-146b-5p and miR-152. Eight circRNAs included in the network were verified, and hsa_circ_0006502 was downregulated in the five tested pancreatic cancer cell lines and functioned as a tumor suppressor, as predicted.Conclusion: In conclusion, a key prognosis-related regulatory network in pancreatic cancers was constructed through bioinformatics analysis of public RNA databases, and miR-146b-5p and miR-152 were the key regulatory factors. Hsa_circ_0006502 was identified as a tumor suppressor that interacted closely with miR-146b-5p and might serve as a potential therapeutic agent.

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“…In addition, miR-146b-5p increases tumor growth by regulating TRAF6 (TNF receptor associated factor 6) and impairs the serum level of IFN-γ. TRAF6 is a signal transducer in the NF-κB pathway, and IFN-γ has been applied to treat patients with ovarian cancer [ 84 ]. Interestingly, KLF6 reduces the localization of p65 and inhibits cancer progression in glioblastoma [ 85 ].…”

Section: Mirnas Act As Oncogenes In Renal Cancermentioning

confidence: 99%

A Review of Recent Research on the Role of MicroRNAs in Renal Cancer

Yang

Zou

et al. 2021

Med Sci Monit

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Renal cell carcinoma (RCC) is a most common type of urologic neoplasms; it accounts for 3% of malignant tumors, with high rates of relapse and mortality. The most common types of renal cancer are clear cell carcinoma (ccRCC), papillary renal cell carcinoma (pRCC), and chromophobe renal carcinoma (chRCC), which account for 90%, 6–15%, and 2–5%, respectively, of all renal malignancies. Although surgical resection, chemotherapy, and radiotherapy are the most common treatment method for those diseases, their effects remain dissatisfactory. Furthermore, recent research shows that the treatment efficacy of checkpoint inhibitors in advanced RCC patients is widely variable. Hence, patients urgently need a new molecular biomarker for early diagnosis and evaluating the prognosis of RCC. MicroRNAs (miRNAs) belong to a family of short, non-coding RNAs that are highly conserved, have long half-life evolution, and post-transcriptionally regulate gene expression; they have been predicted to play crucial roles in tumor metastasis, invasion, angiogenesis, proliferation, apoptosis, epithelial-mesenchymal transition, differentiation, metabolism, cancer occurrence, and treatment resistance. Although some previous papers demonstrated that miRNAs play vital roles in renal cancer, such as pathogenesis, diagnosis, and prognosis, the roles of miRNAs in kidney cancer are still unclear. Therefore, we reviewed studies indexed in PubMed from 2017 to 2020, and found several studies suggesting that there are more than 82 miRNAs involved in renal cancers. The present review describes the current status of miRNAs in RCC and their roles in progression, diagnosis, therapy targeting, and prognosis of RCC.

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Inhibition of miR146b‐5p suppresses CT‐guided renal cell carcinoma by targeting TRAF6

Cited by 11 publications

References 30 publications

miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6

miR‑146b‑5p promotes colorectal cancer progression by targeting TRAF6

Regulatory Network Analysis Reveals Prognosis-Related CircRNAs in Pancreatic Cancer Cells

A Review of Recent Research on the Role of MicroRNAs in Renal Cancer

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