Inhibition of miR‐188‐5p alleviates hepatic fibrosis by significantly reducing the activation and proliferation of HSCs through PTEN/PI3K/AKT pathway

Riaz, Farooq; Chen, Qian; Lu, Kaikai; Osoro, Ezra Kombo; Wu, Linquan; Feng, Lin; Zhao, Rong; Yang, Lu-Yun; Zhou, Yanmin; He, Yingli; Zhu, Li; Du, Xiaohong; Sadiq, Muhammad; Yang, Xudong; Li, Dongmin

doi:10.1111/jcmm.16376

Cited by 37 publications

(26 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that PTEN was predicted to be the target of miR-616-3p and the direct binding relationship was confirmed by dual luciferase assays. PTEN is a tumor-suppressor gene with specific phosphatase activity and plays important roles in cell growth, proliferation, and migration via the PI3K-AKT pathway 30 , 31 . Studies have shown that PTEN expression was decreased both in endometriosis tissue and in primary cultured endometrial stomal cells, which contributed to cell proliferation 32 .…”

Section: Discussionmentioning

confidence: 99%

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis

Yang¹,

Ban²,

Zhang³

et al. 2021

Int. J. Med. Sci.

View full text Add to dashboard Cite

Endometriosis is a common gynecological disease, affecting up to 10% of women of reproductive age and approximately 50% of women with infertility. Circular RNAs (circRNAs) have been shown to be involved in a number of diseases. Dysregulated expression of circRNAs in endometriosis has been reported, and circ_0000673 was significantly downregulated. However, the details of its role in the pathogenesis of endometriosis are still poorly understood. We investigated the location and effects of the downregulation of circ_0000673 in endometriosis. We demonstrated that knockdown of circ_0000673 significantly increased the proliferation and migration of eutopic and normal endometrial cells. Bioinformatics analysis predicted that circ_0000673 might act as a sponge for miR-616-3p. We found that the effect of circ_0000673 knockdown could be recovered by miR-616-3p inhibitor and enhanced by miR-616-3p mimics. qPCR and western blot assays showed that circ_0000673 knockdown could decrease the expression of PTEN and increase the expression of PI3K and p-AKT. PTEN was confirmed to be a target of miR-616-3p. These results demonstrated that the downregulation of circ_0000673 could promote the progression of endometriosis by inactivating PTEN via the deregulation of miR-616-3p.

show abstract

Section: Discussionmentioning

confidence: 99%

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis

Yang¹,

Ban²,

Zhang³

et al. 2021

Int. J. Med. Sci.

View full text Add to dashboard Cite

show abstract

“…We found PTEN was predicted to be the target of miR-616-3p and the direct binding relationship was con rmed by dual luciferase assay. PTEN is a tumor-suppressor gene with speci city phosphatase activity, and plays important roles in cell growth, proliferation and migration via PI3K-AKT pathway [25,26]. Studies showed PTEN expression was decreased in both endometriosis tissue and primary cultured endometrial stomal cells, which contributed to cell proliferation [27].…”

Section: Discussionmentioning

confidence: 99%

Downregulation of Circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis Via Suppressing PTEN

Yang

Ban

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Endometriosis is a prevalent gynecologic disease, affecting up to 10% of women at reproductive age and approximately 50% of women with infertility. The function of circRNAs in various diseases has been highlighted. Dysregulated expression of circRNAs in endometriosis has been reported and circ_0000673 was significantly deregulated. However, its explicit role in pathogenesis of endometriosis is yet to be identified. Methods: circ_0000673 expression was detected in paried ectopic and eutopic endometrium using qPCR and fluorescent in situ hybridization. Knockdown of circ_0000673 in eutopic and normal endometrial stromal cells were done by transfection with lentivirus vectors. The proliferation activity of endometrial stromal cells was evaluated by CCK-8 assay and colony formation assay, while the migration capacity was valued by wound healing assay. PTEN, PI3K and p-AKT were detected by qPCR and western blotting. Dual luciferase assay was performed to assess the bonding between circ_0000673, PTEN and miR-616-3p.Results: The expression of circ_0000673 was reduced in ectopic endometrium. Knockdown of circ_0000673 significantly induced eutopic and normal endometrial cell proliferation and migration. Bioinformatic analysis predicted that circ_0000673 might sponge miR-616-3p. The effect of circ_0000673 knockdown could be recovered by miR-616-3p inhibitor and enhanced by miR-616-3p mimics. Meanwhile, qPCR and western blotting showed that circ_0000673 knockdown inhibited the expression of PTEN, and subsequently activated PI3K and p-Akt. Furthermore, PTEN was confirmed to be a target of miR-616-3p. Conclusion: The results demonstrated that deregulated expression of circ_0000673 could promote endometriosis progression via sponging miR-616-3p and further regulating PTEN.

show abstract

“…miR-129-5p negatively regulates HSC activation induced by paternally expressed gene 3 (PEG3) [ 100 ]. Inhibition of miR-188-5p alleviates liver fibrosis by suppressing HSC activation through the PTEN/PI3K/Akt pathway [ 101 ].…”

Section: Association Of the Causes Of Liver Cirrhosis And Mirnasmentioning

confidence: 99%

Diagnosis and Therapeutic Management of Liver Fibrosis by MicroRNA

Tadokoro

Morishita

Masaki

2021

IJMS

View full text Add to dashboard Cite

Remarkable progress has been made in the treatment and control of hepatitis B and C viral infections. However, fundamental treatments for diseases in which liver fibrosis is a key factor, such as cirrhosis, alcoholic/nonalcoholic steatohepatitis, autoimmune hepatitis, primary biliary cholangitis, and primary sclerosing cholangitis, are still under development and remain an unmet medical need. To solve this problem, it is essential to elucidate the pathogenesis of liver fibrosis in detail from a molecular and cellular perspective and to develop targeted therapeutic agents based on this information. Recently, microRNAs (miRNAs), functional RNAs of 22 nucleotides, have been shown to be involved in the pathogenesis of liver fibrosis. In addition, extracellular vesicles called “exosomes” have been attracting attention, and research is being conducted to establish noninvasive and extremely sensitive biomarkers using miRNAs in exosomes. In this review, we summarize miRNAs directly involved in liver fibrosis, miRNAs associated with diseases leading to liver fibrosis, and miRNAs related to complications of cirrhosis. We will also discuss the efficacy of each miRNA as a biomarker of liver fibrosis and pathology, and its potential application as a therapeutic agent.

show abstract

Inhibition of miR‐188‐5p alleviates hepatic fibrosis by significantly reducing the activation and proliferation of HSCs through PTEN/PI3K/AKT pathway

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 37 publications

References 59 publications

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis

Downregulation of Circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis Via Suppressing PTEN

Diagnosis and Therapeutic Management of Liver Fibrosis by MicroRNA

Contact Info

Product

Resources

About