Inhibition of miR-135a-5p attenuates vascular smooth muscle cell proliferation and vascular remodeling in hypertensive rats

Ye, Chao; Tong, Ying; Wu, Nan; Wan, Guo-Wei; Zheng, Fuqiang; Chen, Jingyu; Lei, Jianzhen; Zhou, Hong; Chen, Aidong; Wang, Juejin; Chen, Qi; Li, Yuehua; Kang, Yu-Ming; Zhu, Guo‐Qing

doi:10.1038/s41401-020-00608-x

Cited by 24 publications

(12 citation statements)

References 37 publications

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“…53 VSMC proliferation greatly contributes to vascular remodeling in hypertension. 54 Second, long-chain LysoPC (C > 16:0) effectively inhibits endothelium-dependent vasodilatation induced by endothelial-derived nitric oxide and hyperpolarizing factors. 55,56 What is more, LysoPC stimulates endothelial ICAM-1 expression, induces transcription of platelet-derived growth factor, and reduces the ability of endothelin, thereby causing the damage to vascular endothelium.…”

Section: Lipid Metabolismmentioning

confidence: 99%

Antihypertensive effect and underlying mechanism of tripeptide NCW on spontaneously hypertensive rats using metabolomics analysis

Zhao

et al. 2022

Food Funct.

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Section: Lipid Metabolismmentioning

confidence: 99%

Antihypertensive effect and underlying mechanism of tripeptide NCW on spontaneously hypertensive rats using metabolomics analysis

Zhao

et al. 2022

Food Funct.

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“…MicroRNAs (miRNAs) are a class of endogenous, non-coding small RNAs (about 18-22 nucleotides) that negatively regulate gene expression by binding to the mRNAs of target genes, specifically on a posttranscriptional level [19]. The dysregulation of miRNAs contributes to the pathogenesis of oxidative stress [20,21], inflammation [22], and several diseases such as cancer [23] and vascular diseases [24,25]. miR-31-5p inhibits the cell migration of renal cell carcinoma [26] and hepatocellular carcinoma [27], but it promotes cell migration in colorectal cancer cells [28].…”

Section: Introductionmentioning

confidence: 99%

miR-31-5p Promotes Oxidative Stress and Vascular Smooth Muscle Cell Migration in Spontaneously Hypertensive Rats via Inhibiting FNDC5 Expression

Zhou

Zheng

et al. 2021

Biomedicines

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Oxidative stress and the migration of vascular smooth muscle cells (VSMCs) are important for vascular remodeling in a variety of vascular diseases. miR-31-5p promotes cell migration in colorectal cancer cells but inhibits cell migration in renal cell carcinoma. However, whether miR-31-5p is involved in oxidative stress and VSMC migration remains unknown. This study shows the crucial roles of miR-31-5p in oxidative stress and VSMC migration, as well as underlying mechanisms. Experiments were carried out in primary VSMCs from aortic media of Wistar–Kyoto rats (WKY) and spontaneously hypertensive rats (SHR), as well as the A7r5 cell line. Oxidative stress was assessed by NADPH oxidase (NOX) expression, NOX activity, and reactive oxygen species (ROS) production. Cell migration was evaluated with a Boyden chamber assay and a wound healing assay. The miR-31-5p mimic and inhibitor promoted and attenuated oxidative stress and cell migration in the VSMCs of SHR, respectively. A dual-luciferase reporter assay indicated that miR-31-5p targeted the 3’UTR domain of FNDC5. The miR-31-5p level was raised and FNDC5 expression was reduced in the VSMCs of SHR compared with those of WKY. The miR-31-5p mimic reduced FNDC5 expression in the A7r5 cells and the VSMCs of both WKY and SHR, while the miR-31-5p inhibitor only increased FNDC5 expression in the VSMCs of SHR. Exogenous FNDC5 attenuated not only the oxidative stress and VSMC migration in SHR but also the roles of the miR-31-5p mimic in inducing oxidative stress and VSMC migration. These results indicate that miR-31-5p promotes oxidative stress and VSMC migration in SHR via inhibiting FNDC5 expression. The increased miR-31-5p and reduced FNDC5 in the VSMCs of SHR contribute to enhanced oxidative stress and cell migration.

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“…Immunohistochemistry analysis was performed as we previously reported [ 31 , 32 ] Brain sections were respectively performed at the level of iDMV. Immunohistochemistry for salusin-β was made.…”

Section: Methodsmentioning

confidence: 99%

Salusin-β in Intermediate Dorsal Motor Nucleus of the Vagus Regulates Sympathetic-Parasympathetic Balance and Blood Pressure

Zheng

et al. 2021

Biomedicines

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The dorsal motor nucleus of the vagus (DMV) is known to control vagal activity. It is unknown whether the DMV regulates sympathetic activity and whether salusin-β in the DMV contributes to autonomic nervous activity. We investigated the roles of salusin-β in DMV in regulating sympathetic-parasympathetic balance and its underline mechanisms. Microinjections were carried out in the DMV and hypothalamic paraventricular nucleus (PVN) in male adult anesthetized rats. Renal sympathetic nerve activity (RSNA), blood pressure and heart rate were recorded. Immunohistochemistry for salusin-β and reactive oxidative species (ROS) production in the DMV were examined. Salusin-β was expressed in the intermediate DMV (iDMV). Salusin-β in the iDMV not only inhibited RSNA but also enhanced vagal activity and thereby reduced blood pressure and heart rate. The roles of salusin-β in causing vagal activation were mediated by NAD(P)H oxidase-dependent superoxide anion production in the iDMV. The roles of salusin-β in inhibiting RSNA were mediated by not only the NAD(P)H oxidase-originated superoxide anion production in the iDMV but also the γ-aminobutyric acid (GABA)A receptor activation in PVN. Moreover, endogenous salusin-β and ROS production in the iDMV play a tonic role in inhibiting RSNA. These results indicate that salusin-β in the iDMV inhibits sympathetic activity and enhances vagal activity, and thereby reduces blood pressure and heart rate, which are mediated by NAD(P)H oxidase-dependent ROS production in the iDMV. Moreover, GABAA receptor in the PVN mediates the effect of salusin-β on sympathetic inhibition. Endogenous salusin-β and ROS production in the iDMV play a tonic role in inhibiting sympathetic activity.

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Inhibition of miR-135a-5p attenuates vascular smooth muscle cell proliferation and vascular remodeling in hypertensive rats

Cited by 24 publications

References 37 publications

Antihypertensive effect and underlying mechanism of tripeptide NCW on spontaneously hypertensive rats using metabolomics analysis

Antihypertensive effect and underlying mechanism of tripeptide NCW on spontaneously hypertensive rats using metabolomics analysis

miR-31-5p Promotes Oxidative Stress and Vascular Smooth Muscle Cell Migration in Spontaneously Hypertensive Rats via Inhibiting FNDC5 Expression

Salusin-β in Intermediate Dorsal Motor Nucleus of the Vagus Regulates Sympathetic-Parasympathetic Balance and Blood Pressure

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