Inhibition of microbial adhesion to plastic surface and human buccal epithelial cells by Rhodomyrtus tomentosa leaf extract

Limsuwan, Surasak; Homlaead, Sirilux; Watcharakul, Sirimaporn; Chusri, Sasitorn; Moosigapong, Kotchakorn; Saising, Jongkon; Voravuthikunchai, Supayang Piyawan

doi:10.1016/j.archoralbio.2014.07.017

Cited by 23 publications

(11 citation statements)

References 48 publications

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“…The ethanol extract of R. tomentosa leaves had profound antibacterial activity against all staphylococcal bacteria isolated from milk with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranged from 16 to 64 μg/mL and from 64 to128 μg/mL, respectively [63]. It also exhibited antibacterial activity against S. aureus ATCC 25923, Streptococcus mutans , and C. albicans ATCC 90028 with MIC values of 31.25, 15.62, and 1000 µg/mL, respectively [64]. Moreover, this extract effectively inhibited Streptococcus agalactiae and Streptococcus iniae isolated from infected tilapia with MIC values ranging from 7.8 to 62.5 µg/mL.…”

Section: Pharmaceutical Propertiesmentioning

confidence: 99%

The Health Beneficial Properties of Rhodomyrtus tomentosa as Potential Functional Food

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2019

Biomolecules

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Rhodomyrtus tomentosa (Aiton) Hassk. is a flowering plant belonging to the family Myrtaceae, native to southern and southeastern Asia. It has been used in traditional Vietnamese, Chinese, and Malaysian medicine for a long time for the treatment of diarrhea, dysentery, gynecopathy, stomachache, and wound healing. Moreover, R. tomentosa is used to make various food products such as wine, tea, and jam. Notably, R. tomentosa has been known to contain structurally diverse and biologically active metabolites, thus serving as a potential resource for exploring novel functional agents. Up to now, numerous phenolic and terpenoid compounds from the leaves, root, or fruits of R. tomentosa have been identified, and their biological activities such as antioxidant, antibacterial, anti-inflammatory, and anticancer have been evidenced. In this contribution, an overview of R. tomentosa and its health beneficial properties was focused on and emphasized.

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Section: Pharmaceutical Propertiesmentioning

confidence: 99%

The Health Beneficial Properties of Rhodomyrtus tomentosa as Potential Functional Food

Ngo

2019

Biomolecules

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“…Rhodomyrtone did also not cause skin irritation upon topical application in rabbits [ 10 ] and acute toxicity tests did not show adverse effects in mice, when injected [ 11 ]. Furthermore, it has been proven effective against Propionibacterium acne biofilms [ 10 , 12 ], showed excellent results in preventing staphylococcal adhesion and invasion in a tissue model of bovine mastitis [ 13 ], and prevented adhesion of dental pathogens to plastic surfaces and human buccal cells [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

The novel antibiotic rhodomyrtone traps membrane proteins in vesicles with increased fluidity

et al. 2018

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The acylphloroglucinol rhodomyrtone is a promising new antibiotic isolated from the rose myrtle Rhodomyrtus tomentosa, a plant used in Asian traditional medicine. While many studies have demonstrated its antibacterial potential in a variety of clinical applications, very little is known about the mechanism of action of rhodomyrtone. Preceding studies have been focused on intracellular targets, but no specific intracellular protein could be confirmed as main target. Using live cell, high-resolution, and electron microscopy we demonstrate that rhodomyrtone causes large membrane invaginations with a dramatic increase in fluidity, which attract a broad range of membrane proteins. Invaginations then form intracellular vesicles, thereby trapping these proteins. Aberrant protein localization impairs several cellular functions, including the respiratory chain and the ATP synthase complex. Being uncharged and devoid of a particular amphipathic structure, rhodomyrtone did not seem to be a typical membrane-inserting molecule. In fact, molecular dynamics simulations showed that instead of inserting into the bilayer, rhodomyrtone transiently binds to phospholipid head groups and causes distortion of lipid packing, providing explanations for membrane fluidization and induction of membrane curvature. Both its transient binding mode and its ability to form protein-trapping membrane vesicles are unique, making it an attractive new antibiotic candidate with a novel mechanism of action.

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“…Pseudomonas.aeruginosa ATCC 9027 were cultured in liquid BHI media, incubated at 37 ° C for 24 hours, washed by adding 0.9% NaCl, then centrifuged 2 times for 10 minutes (5,000 rpm) (Wolska et al, 2005). NaCl solution was added to achieve 0.5 McFarland standard or 10 8 CFU/ml of bacterial concentration (Limsuwan et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Effect of Red Onion (Allium cepa var ascalonicum) Skin Ethanolic Extract on the Motility and the Adhesion Index of Pseudomonas aeruginosa and Macrophage Phagocytosis Index

et al. 2019

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Red onion skin (Allium cepa var ascalonicum) contains various ingredients that may function as antibacterial agents against microorganisms, as well as anti-inflammatory and immunomodulator agents for host cells, such as macrophages. Pseudomonas aeruginosa found in the oral cavity is commensal bacteria that may turn into opportunistic pathogen by utilizing its virulence factors such as motility and adhesion to the host cell. The purpose of this study was to investigate the effect of red-onion-skin ethanolic extract towards P. aeruginosa ATCC 9027 on the motility and adhesion ability, furthermore, to know its effect on the macrophage phagocytosis. The research was conducted into three parts of experiment using red-onionskin ethanolic extract. Extract-induced bacterial motility test was carried out on semi-solid media, stained using 0.1% crystal violet, then the radial length of the bacterial movement was measured. The bacterial adhesion index to buccal cells was calculated after incubated for two hours and stained with Gram stain. Phagocytic activity of the host cells on P. aeruginosa was done by exposing the extract to the mouse peritoneal macrophages, then the phagocytosed bacteria were counted after Giemsa staining. Statistical test results from the three experiments showed significant differences between the test groups compared to the control groups (p <0.05). It was concluded that the red onion-skin ethanolic extract not only affects P. aeruginosa by reducing swarming motility and preventing bacterial adhesion to buccal epithelial cells, but also induces the host cells by increasing the ability of macrophage phagocytosis to these bacteria.

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Inhibition of microbial adhesion to plastic surface and human buccal epithelial cells by Rhodomyrtus tomentosa leaf extract

Cited by 23 publications

References 48 publications

The Health Beneficial Properties of Rhodomyrtus tomentosa as Potential Functional Food

The Health Beneficial Properties of Rhodomyrtus tomentosa as Potential Functional Food

The novel antibiotic rhodomyrtone traps membrane proteins in vesicles with increased fluidity

Effect of Red Onion (Allium cepa var ascalonicum) Skin Ethanolic Extract on the Motility and the Adhesion Index of Pseudomonas aeruginosa and Macrophage Phagocytosis Index

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