Inhibition of Methanol Extract from the Aerial Parts of Saururus chinensis on Lipopolysaccharide-Induced Nitric Oxide and Prostagladin E2 Production from Murine Macrophage RAW 264.7 Cells.

Abstract

As an attempt to search for bioactive natural products exerting antiinflammatory activity, we have evaluated the effects of the methanol extract from the aerial parts of Saururus chinensis (LOUR.) BAILL (Saururaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) release by the macrophage cell line RAW 264.7. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased PGE 2 release. Consistent with these observations, the protein and mRNA expression level of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 was inhibited by MeOH extracts of the aerial part of S. chinensis (SCM) in a dose-dependent manner. Furthermore, SCM inhibited the LPS-induced DNA binding activity of nuclear factor-k kB (NF-k kB), which was associated with decreased p65 protein levels in the nucleus. These results suggest that SCM inhibits LPS-induced iNOS and COX-2 expression by blocking NF-k kB activation.

Potent Anti-inflammatory and Analgesic Actions of the Chloroform Extract of <i>Dendropanax morbifera</i> Mediated by the Nrf2/HO-1 Pathway

“…31,32) For iNOS enzymatic reaction, the cells were stimulated with LPS for 12 h followed by two washes with phosphate buffered saline (PBS). These pre-stimulated cells were incubated in the presence or absence of DP-C for a further 12 h without LPS.…”

Protective Effects of Manassantin A against Ethanol-Induced Gastric Injury in Rats

“…Its aerial portion has been used in Korean folk medicine for the treatment of edema, gonorrhea, jaundice, and several inflammatory diseases. 10) Studies have shown that S. chinensis has various activities, including anti-inflammatory, anti-oxidant, 11,12) antiangiogenic, 13) anti-asthmatic, 14) and anti-hypertensive 15) activities, as well as therapeutic activity against atopic dermatitis. 16) Among the bioactive compounds isolated from S. chinensis, manassantin A is a major phytochemical composition that has various biological activities, including anti-inflammatory, neuroleptic, and human acyl-CoA : cholesterol acyltransferase (ACA T) inhibitory activities.…”

Saururus chinensis Baill induces apoptosis through endoplasmic reticulum stress in HepG2 hepatocellular carcinoma cells

“…Several studies have reported that the ethanol extract of S. chinensis Baill (SC-E) decreased inflammation by inhibiting intracellular nitric oxide, prostaglandin E2, and various inflammatory cytokines released by lipopolysaccharide stimulation of raw 264.7 macrophages (Kim et al, 2003;Yoo et al, 2008;. SC-E also regulated blood lipid level in animal model and suppressed the activity of a-glucosidase for the anti-diabetic effect (Hwang et al, 2007;Yu et al, 2008).…”