“…Natural polysaccharides possess many beneficial health properties. Recent research has indicated that polysaccharides and their sulphated derivative are able to activate many cell signaling events that closely correlate with tumor development (30)(31)(32)(33). Therefore, polysaccharides from medicinal plants may be a resource repository for the search for novel therapeutic agents against cancer.…”
In a previous study, our team preliminarily investigated the bioefficacy of an extracted polysaccharide from the medicinal plant Aconitum coreanum (ACP1). In the present study, we further evaluated the antitumor efficacy of an ACP1 sulphated derivative (ACP1‑s) in the human brain glioblastoma U87MG cell line. Cell viability assay and flow cytometry results demonstrated that 400, 800 and 1,600 µg/ml ACP1‑s induced cell growth inhibition and cell apoptosis. We then investigated the underlying molecular mechanism of the ACP1‑s induced cell apoptosis and found that the NF‑κB/Bcl‑2 cell apoptotic signaling pathway was involved. Following treatment with ACP1‑s, the expression of IκB in U87MG cells was significantly upregulated, whereas the level of NF‑κB and the ratio of Bcl‑2/Bax was significantly decreased. The level of cleaved caspase‑3 was increased accordingly. When we introduced exogenous p65 protein into the U87MG cells, the ACP1‑s-induced cell growth inhibition and cell apoptosis were partially neutralized, and the expression of the anti‑apoptotic gene Bcl‑2 was recovered accordingly. These findings suggest the potential value of ACP1‑s as a novel therapeutic agent for the treatment of glioblastoma.
“…Natural polysaccharides possess many beneficial health properties. Recent research has indicated that polysaccharides and their sulphated derivative are able to activate many cell signaling events that closely correlate with tumor development (30)(31)(32)(33). Therefore, polysaccharides from medicinal plants may be a resource repository for the search for novel therapeutic agents against cancer.…”
In a previous study, our team preliminarily investigated the bioefficacy of an extracted polysaccharide from the medicinal plant Aconitum coreanum (ACP1). In the present study, we further evaluated the antitumor efficacy of an ACP1 sulphated derivative (ACP1‑s) in the human brain glioblastoma U87MG cell line. Cell viability assay and flow cytometry results demonstrated that 400, 800 and 1,600 µg/ml ACP1‑s induced cell growth inhibition and cell apoptosis. We then investigated the underlying molecular mechanism of the ACP1‑s induced cell apoptosis and found that the NF‑κB/Bcl‑2 cell apoptotic signaling pathway was involved. Following treatment with ACP1‑s, the expression of IκB in U87MG cells was significantly upregulated, whereas the level of NF‑κB and the ratio of Bcl‑2/Bax was significantly decreased. The level of cleaved caspase‑3 was increased accordingly. When we introduced exogenous p65 protein into the U87MG cells, the ACP1‑s-induced cell growth inhibition and cell apoptosis were partially neutralized, and the expression of the anti‑apoptotic gene Bcl‑2 was recovered accordingly. These findings suggest the potential value of ACP1‑s as a novel therapeutic agent for the treatment of glioblastoma.
“…The antitumor activity of polysaccharides from various medicinal plants has been demonstrated in ovarian cancer cells, which is mostly associated with the inhibition of cell proliferation, migration and invasion, and the induction of cell apoptosis [ 28 , 29 ]. Se has also been found to play a role in prevention and treatment of ovarian cancer [ 30 , 31 ].…”
Polysaccharides from medicinal plants exert antitumor activity in many cancers. Our previous study demonstrated that polysaccharides extracted from the selenium-enriched Pyracantha fortuneana (Se-PFPs) showed antiproliferative effect in breast cancer cell line. This study aimed to investigate the antitumor effect of Se-PFPs in ovarian cancer cells in vitro and in vivo. Se-PFPs could decrease cell viability, induce apoptosis, and inhibit migratory and invasive potentials in HEY and SKOV3 cells. These findings are supported by reduced expression of cyclin D1, Bcl-2 and MMP-9, enhanced cleavage of PARP and caspase-3, elevated activity of caspase-3 and caspase-9, and EMT (epithelial to mesenchymal transition) inhibition (elevated expression of E-cadherin and cytokeratin 19, and reduced expression of N-cadherin, vimentin, ZEB1 and ZEB2). Moreover, Se-PFPs inhibited xenografted tumor growth through inhibiting cell proliferation and inducing cell apoptosis. More importantly, Se-PFPs significantly reduced cytoplasmic β-catenin particularly nuclear β-catenin expression but increased β-catenin phosphorylation in a GSK-3β-dependent mechanism. Furthermore, β-catenin knockdown exerted similar effects on cell proliferation and invasion as seen in Se-PFPs-treated cells, while β-catenin overexpression neutralized the inhibitory effects of Se-PFPs on cell proliferation and invasion. Take together,Se-PFPs exert antitumor activity through inhibiting cell proliferation, migration, invasion and EMT, and inducing cell apoptosis. These effects are achieved by the inhibition of β-catenin signaling. Thus Se-PFPs can be used as potential therapeutic agents in the prevention and treatment of ovarian cancer.
“…It is rich in polyphenols, flavonoids, organic acids, triterpenes, amino acids, polysaccharides and essential oils [23,24,25]. All these elements allow R. roxburghii to deliver a range of health benefits including anti-oxidation, anti-mutagenesis, anti-inflammation, anti-aging and anti-tumor effects [26,27,28,29]. Notably, R. roxburghii has been proved to enhance the expression of anti-oxidative, DNA mismatch repair, and telomere maintenance (i.e., XRCC5) genes in macrophage cells, meaning that R. roxburghii can improve the anti-oxidative capability, reduce the possibility of cancer formation, and prolong the life span in cells [30].…”
This research unveils potential of the Rosa roxburghii formula drink for anti-oxidation, anti-aging, anti-inflammation, and the reduction of cancer risk. We recruited 40 high risk subjects (i.e., chronic smoker or drinker) and required them to have daily supplement of R. roxburghii formula drink over 12 weeks to investigate the long-term effect on health improvement. We discovered that the drink could increase mitochondrial activity (elevated nicotinamide adenine dinucleotide (NADH) level), alleviate inflammatory response by the reduction of tumor necrosis factor alpha (TNF-α) level, and diminish the expression of cancer biomarkers of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and carcinoembryonic antigen (CEA). As a result, this is the first study to elucidate the synergistic effect of R.roxburghii and other five kinds of fruits on alleviation oxidative damages.
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