Inhibition of MCF-7 breast cancer cell proliferation by a synthetic peptide derived from the C-terminal sequence of Orai channel

Li, Shan; Yao, Meilian; Niu, Chengqun; Liu, Dan; Tang, Zhiming; Gu, Chunming; Zhao, Hongyan; Jin, Ke; Wu, Shengying; Wang, Xiong; Wu, Fuyun

doi:10.1016/j.bbrc.2019.06.153

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Changes in the cytosolic Ca 2+ concentration ([Ca 2+ ] c ) due to Ca 2+ entry across the plasma membrane have been reported to be involved in cell proliferation [1][2][3][4]. In fact, several cancer cell lines present altered [Ca 2+ ] c due to dysregulation of Ca 2+ entry mechanisms, among others [3,[5][6][7][8][9]. The Ca 2+ currents I SOC , I CRAC , and I ARC were described as relevant inward Ca 2+ currents in non-excitable cells including several cancer cell types [10,11].…”

Section: Introductionmentioning

confidence: 99%

Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry

Cantonero

Sánchez-Collado

López

et al. 2020

IJMS

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Arachidonic acid (AA) is a phospholipase A2 metabolite that has been reported to mediate a plethora of cellular mechanisms involved in healthy and pathological states such as platelet aggregation, lymphocyte activation, and tissue inflammation. AA has been described to activate Ca2+ entry through the arachidonate-regulated Ca2+-selective channels (ARC channels). Here, the analysis of the changes in the intracellular Ca2+ homeostasis revealed that, despite MDA-MB-231 cells expressing the ARC channel components Orai1, Orai3, and STIM1, AA does not evoke Ca2+ entry in these cells. We observed that AA evokes Ca2+ entry in MDA-MB-231 cells transiently expressing ARC channels. Nevertheless, MDA-MB-231 cell treatment with AA reduces cell proliferation and migration while inducing cell death through apoptosis. The latter mostly likely occurs via mitochondria membrane depolarization and the activation of caspases-3, -8, and -9. Altogether, our results indicate that AA exerts anti-tumoral effects on MDA-MB-231 cells, without having any effect on non-tumoral breast epithelial cells, by a mechanism that is independent on the activation of Ca2+ influx via ARC channels.

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Section: Introductionmentioning

confidence: 99%

Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry

Cantonero

Sánchez-Collado

López

et al. 2020

IJMS

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“…From the studies of dOrai, it is well established that six TM1 units form the inner wall of the ORAI channel, while the other three TMs are sequentially layered from inside to outside, with TM4 on the outermost side (Figure 1B,C). Given that ORAI is a key component of the Ca 2+ release-activated Ca 2+ (CRAC) channel, abnormal alterations in ORAI would lead to Ca 2+ dysregulation and subsequentially cause various pathological conditions, including immune diseases [13], cardiovascular diseases [19], as well as cancers [20][21][22]. In the present work, we aim to elucidate the activation and modulation mechanism of the ORAI channel, as well as to summarize the latest research on ORAI-related diseases.…”

Section: Introductionmentioning

confidence: 99%

ORAI Ca2+ Channels in Cancers and Therapeutic Interventions

Zhang,

Wang,

2024

Biomolecules

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The ORAI proteins serve as crucial pore-forming subunits of calcium-release-activated calcium (CRAC) channels, pivotal in regulating downstream calcium-related signaling pathways. Dysregulated calcium homeostasis arising from mutations and post-translational modifications in ORAI can lead to immune disorders, myopathy, cardiovascular diseases, and even cancers. Small molecules targeting ORAI present an approach for calcium signaling modulation. Moreover, emerging techniques like optogenetics and optochemistry aim to offer more precise regulation of ORAI. This review focuses on the role of ORAI in cancers, providing a concise overview of their significance in the initiation and progression of cancers. Additionally, it highlights state-of-the-art techniques for ORAI channel modulation, including advanced optical tools, potent pharmacological inhibitors, and antibodies. These novel strategies offer promising avenues for the functional regulation of ORAI in research and may inspire innovative approaches to cancer therapy targeting ORAI.

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“…The presence of tumor immunogenicity in the breast cancer microenvironment has necessitated the use of immunotherapy as a potential cancer treatment [ 25 , 26 ]. Immunotherapy can target specific cells that are involved in hijacking the immune cells; thus, it seems to be a good idea for the therapy’s success.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, an understanding of the immune cell population may have significant consequences for the prevention of breast cancer, enhanced risk management strategies, and the control of breast carcinogenesis. The presence of tumor immunogenicity in the breast cancer microenvironment has necessitated the use of immunotherapy as a potential cancer treatment [25,26]. Immunotherapy can target specific cells that are involved in hijacking the immune cells; thus, it seems to be a good idea for the therapy's success.…”

Section: Introductionmentioning

confidence: 99%

Peptide-Based Vaccine against Breast Cancer: Recent Advances and Prospects

et al. 2023

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Breast cancer is considered the second-leading cancer after lung cancer and is the most prevalent cancer among women globally. Currently, cancer immunotherapy via vaccine has gained great attention due to specific and targeted immune cell activity that creates a potent immune response, thus providing long-lasting protection against the disease. Despite peptides being very susceptible to enzymatic degradation and poor immunogenicity, they can be easily customized with selected epitopes to induce a specific immune response and particulate with carriers to improve their delivery and thus overcome their weaknesses. With advances in nanotechnology, the peptide-based vaccine could incorporate other components, thereby modulating the immune system response against breast cancer. Considering that peptide-based vaccines seem to show remarkably promising outcomes against cancer, this review focuses on and provides a specific view of peptide-based vaccines used against breast cancer. Here, we discuss the benefits associated with a peptide-based vaccine, which can be a mainstay in the prevention and recurrence of breast cancer. Additionally, we also report the results of recent trials as well as plausible prospects for nanotechnology against breast cancer.

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Inhibition of MCF-7 breast cancer cell proliferation by a synthetic peptide derived from the C-terminal sequence of Orai channel

Cited by 6 publications

References 24 publications

Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry

Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry

ORAI Ca2+ Channels in Cancers and Therapeutic Interventions

Peptide-Based Vaccine against Breast Cancer: Recent Advances and Prospects

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