Inhibition of <em>E. coli</em> and <em>S. aureus</em> with selenium nanoparticles synthesized by pulsed laser ablation in deionized water

Abstract

Nosocomial diseases are mainly caused by two common pathogens, Escherichia coli and Staphylococcus aureus, which are becoming more and more resistant to conventional antibiotics. Therefore, it is becoming increasingly necessary to find other alternative treatments than commonly utilized drugs. A promising strategy is to use nanomaterials such as selenium nanoparticles. However, the ability to produce nanoparticles free of any contamination is very challenging, especially for nano-medical applications. This paper reports the successful synthesis of pure selenium nanoparticles by laser ablation in water and determines the minimal concentration required for ~50% inhibition of either E. coli or S. aureus after 24 hours to be at least ~50 ppm. Total inhibition of E. coli and S. aureus is expected to occur at 107±12 and 79±4 ppm, respectively. In this manner, this study reports for the first time an easy synthesis process for creating pure selenium to inhibit bacterial growth.

SeNPs/PLGA Inhibits Biofilm Formation by Cultivable Oral Bacteria Isolated from Caries-Active Children

“…However, MIC 90 of SeNP sagainst the 3 Gram-negative strains belonged to the genus Prevotella was higher than those was recorded against Grampositive in the present study. This may be due to the presence of an extra layer of lipopolysaccharide and proteins (outer membrane), as a part of cell wall of Gramnegative bacteria (Guisbiers et al, 2016). In general, SeNPs applied in the present study showed high inhibition potential against Gram-positive bacteria compared with their efficiency against Gram-negative bacteria.…”

Inhibition of Candida albicans biofilm by pure selenium nanoparticles synthesized by pulsed laser ablation in liquids

“…Indeed, C. albicans form generally a biofilm that protects themselves from the immune system(52). Therefore, the strategy is to apply the pure Se NPs synthesized by PLAL on the biofilm produced by this fungus as selenium is known for its anti-bacterial properties(6, 17, 28). The inhibition was tested following a well-known phenotypic assay(23, 33).…”

“…Furthermore, it has been shown that Se NPs can also affect other organisms. In particular they prevent the growth and proliferation of E. coli and S. aureus , the two bacteria that are mainly responsible of nosocomial diseases(6, 17), also called hospital-acquired infections (HAI), i.e. those occurring within 48 hours of hospital admission, 3 days of discharge or 30 days after an operation.…”

Macrophage‐Targeted Isoniazid–Selenium Nanoparticles Promote Antimicrobial Immunity and Synergize Bactericidal Destruction of Tuberculosis Bacilli

“…aureus, and S . epidermidis , and protect against bacterium‐mediated tissue injury . Concurrently, as macrophages highly express mannose receptors, mannose‐decorated nanoparticles can serve as excellent carriers delivering drugs into macrophages .…”