Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored pectins

Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following <em>Porphyromonas gingivalis</em> infection

“…We found that there were more proliferating cells in different time points on the surfaces coated with PA compared to control TCPS surfaces, which indicates the influence of RG-Is on osteoblasts' activation. Similar results have been reported by Gallet et al 32 with murine macrophage cell line J774.2 cultured on pectin MHR-α (modified hairy regions) from apple in the presence of bacterial LPSs. The MHR-α nanocoating inhibits the LPS-induced activation of macrophages.…”

“…This indicated that RG-Is, especially pectin substratum with lower arabinose content, significantly inhibit the expression of inflammatory mediators in osteoblasts infected with P. gingivalis. This is in line with a study by Gallet et al 32 using a murine macrophage cell line J774.2 cultured on pectin MHR-α from apple and stimulated by bacterial LPSs. The MHR-α substratum significantly inhibits the secretion of proinflammatory TNF-α mediator and generation of nitrous oxide by LPS-activated macrophages.…”

Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

“…Most studies investigating periodontal and peri‐implant diseases indicate a loss of soft connective tissue and bone integrity as a result of the host inflammatory response to the bacterial infection . Pectin polysaccharides, including RG‐I, have been shown to have immunomodulatory properties, which may reduce the risk of peri‐implantitis …”

“…These findings are supported by results obtained with murine macrophage cell line J774.2 cultured on modified hairy region‐α (MHR‐α) from apple pectin in the presence of the bacterial virulence factor lipopolysaccharide (LPS). The MHR‐α nanocoating prevents the decrease in the proliferation and cell viability of LPS‐activated macrophages . A number of studies investigating the destructive mechanisms of periodontal pathogens have shown that immune response is directly responsible for connective tissue breakdown and bone loss .…”

“…A number of studies have shown that plant‐derived polysaccharides are immunomodulatory agents . Recently, it was reported that pectin polysaccharide rhamnogalacturonan‐I (RG‐I) can prevent inflammation, and improve tissue regeneration .…”