Inhibition of Listeria monocytogenes using biofilms of non-pathogenic soil bacteria (Streptomyces spp.) on stainless steel under desiccated condition

Kim, Yoonbin; Kim, Hoikyung; Beuchat, Larry R.; Ryu, Jee Hoon

doi:10.1016/j.fm.2018.11.007

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…After that, samples were incubated at 45°C for 0, 30, 60, 90, 120, or 180 min. At each time point, 1 g of sterile glass beads (1 mm, Glastechnique Mfg., Germany) was added into the tube and vortexed for 5 min ( Kim et al, 2019 ; Huang et al, 2020 ). The resulting suspension was serially diluted with sterile PBS, followed by plating spread, incubation, and enumeration.…”

Section: Methodsmentioning

confidence: 99%

Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms

et al. 2021

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Alicyclobacillus acidoterrestris (A. acidoterrestris), a spore-forming bacterium, has become a main challenge and concern for the juices and acid beverage industry across the world due to its thermo-acidophilic characteristic. Thymoquinone (TQ) is one of the active components derived from Nigella sativa seeds. The objective of this study was to investigate antibacterial activity and associated molecular mechanism of TQ against A. acidoterrestris vegetative cells, and to evaluate effects of TQ on A. acidoterrestris spores and biofilms formed on polystyrene and stainless steel surfaces. Minimum inhibitory concentrations of TQ against five tested A. acidoterrestris strains ranged from 32 to 64 μg/mL. TQ could destroy bacterial cell morphology and membrane integrity in a concentration-dependent manner. Field-emission scanning electron microscopy observation showed that TQ caused abnormal morphology of spores and thus exerted a killing effect on spores. Moreover, TQ was effective in inactivating and removing A. acidoterrestris mature biofilms. These findings indicated that TQ is promising as a new alternative to control A. acidoterrestris and thereby reduce associated contamination and deterioration in the juice and acid beverage industry.

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Section: Methodsmentioning

confidence: 99%

Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms

et al. 2021

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“…Methodologies developed based on the interaction of species to eliminate or inhibit the growth of L. monocytogenes in food-processing environments (Leriche et al, 1999;Zhao et al, 2004Zhao et al, , 2006 include the use of Listeria-specific reagents such as Listeria phages and their derivatives (endolysins, bacteriocins) (Ganegama Arachchi et al, 2013;Hagens and Loessner, 2014;Gutiérrez et al, 2016;Kim et al, 2019). A recent study showed that bacteriocin pentocin MQ1 isolated from Lactobacillus pentosus CS2 has a biopreservative potential and presented antibacterial activity against L. monocytogenes and B. cereus (Wayah and Philip, 2018).…”

Section: Introductionmentioning

confidence: 99%

Competitive and/or Cooperative Interactions of Listeria monocytogenes With Bacillus cereus in Dual-Species Biofilm Formation

2020

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Microorganisms in dairy industries can form monospecies, dual-species, or multispecies biofilms, showing cooperative or competitive behaviors, which might contribute to the reduction of efficiency of cleaning and sanitization processes and eventually turn into a potential source of contamination. This study proposes to evaluate the behavior of Listeria monocytogenes in monospecies biofilms, cocultured with Bacillus cereus. The isolates were of dairy origin, and the selection occurred after studies of competition among species. The biofilm formations on AISI 304 stainless steel at 25 • C in a stationary culture were analyzed to observe the cooperative or competitive interactions among species, as well as the effect of pre-adhered cells. Biofilm formation assays were performed in four experiments: Experiment 1: in the presence of strains of antagonistic substance producer B. cereus (+); Experiment 2: extract of the antagonistic substance of B. cereus; Experiment 3: pre-adhered cells of B. cereus; and Experiment 4: pre-adhered cells of L. monocytogenes. Subsequently, cooperative behavior was observed by scanning electron microscopy. The L. monocytogenes monospecies biofilm counts of greater than 5 log colony-forming units (CFU)/cm 2 were also observed in dual-species biofilms in the presence of B. cereus (non-producers of antagonist substance), showing cooperative behavior between species. However, in the presence of antagonistic substance produced by B. cereus, the counts were lower, 1.39 and 1.70 log CFU/cm 2 (p > 0.05), indicating that the antagonistic substance contributes to competitive interactions. These data are relevant for the development of new studies to control L. monocytogenes in the dairy industry.

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“…Briefly, the sheets were immersed in a test tube with ∼20 mL of detergent solution and sonicated for 30 min. Then, the sheets were rinsed with sterile deionized water several times to dissipate any remaining material, and were transferred into 20 mL of 70% (v/v) ethanol and sonicated for another 15 min (Kim et al, 2019). After cleaning, the sheets were air-dried and sterilized in a high temperature autoclave for 15 min before use.…”

Section: Cleaning Of the 304 Stainless Steel Sheetsmentioning

confidence: 99%

“…For the LED-treated groups, the rinsed sheets in fixer were illuminated at 25, 10, and 4 • C, respectively, while the rinsed sheets in fixer of the non-LED controls were placed in an incubator at 29.5, 15.5, and 12.5 • C, protected from the light. At the specified times (0, 30, 60, 120, and 240 min), the sheets were transferred into 50 mL centrifuge tubes containing 3 g of glass beads (425-600 µm; Sigma-Aldrich, St. Louis, MO, USA) and 30 mL PBS (Kim et al, 2019). After vortexing for 5 min, the detached bacterial suspension was serially diluted in PBS, then 0.1 mL was spreadplated onto TSA plates and incubated at 37 • C for 24 h. Survival cell counts were expressed as log CFU/cm 2 .…”

Section: Population Of Surviving Biofilm Cells After Led Illuminationmentioning

confidence: 99%

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

et al. 2020

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The objectives of this study were to evaluate the inactivation efficacy of a 405-nm light-emitting diode (LED) against Cronobacter sakazakii biofilm formed on stainless steel and to determine the sensitivity change of illuminated biofilm to food industrial disinfectants. The results showed that LED illumination significantly reduced the population of viable biofilm cells, showing reduction of 2.0 log (25°C), 2.5 log (10°C), and 2.0 log (4°C) between the non-illuminated and LED-illuminated groups at 4 h. Images of confocal laser scanning microscopy and scanning electron microscopy revealed the architectural damage to the biofilm caused by LED illumination, which involved destruction of the stereoscopic conformation of the biofilm. Moreover, the loss of biofilm components (mainly polysaccharide and protein) was revealed by attenuated total reflection Fourier-transformed infrared spectroscopy, and the downregulation of genes involved in C. sakazakii biofilm formation was confirmed by real time quantitative PCR analysis, with greatest difference observed in fliD. In addition, the sensitivity of illuminated-biofilm cells to disinfectant treatment was found to significantly increased, showing the greatest sensitivity change with 1.5 log reduction between non-LED and LED treatment biofilms in the CHX-treated group. These results indicated that 405 nm LED illumination was effective at inactivating C. sakazakii biofilm adhering to stainless steel. Therefore, the present study suggests the potential of 405 nm LED technology in controlling C. sakazakii biofilms in food processing and storage, minimizing the risk of contamination.

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Inhibition of Listeria monocytogenes using biofilms of non-pathogenic soil bacteria (Streptomyces spp.) on stainless steel under desiccated condition

Cited by 10 publications

References 20 publications

Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms

Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms

Competitive and/or Cooperative Interactions of Listeria monocytogenes With Bacillus cereus in Dual-Species Biofilm Formation

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

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