Inhibition of lipopolysaccharide-induced neuroinflammatory events in Bv-2 microglia by chestnut peel extract

Kang, Hunseung

doi:10.4314/tjpr.v13i10.7

Cited by 4 publications

(4 citation statements)

References 18 publications

(25 reference statements)

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“…Cerulli and co-workers showed that the pre-treatment of THP1 and THP-1-XBLUE-MD2-CD14 cells with extracts of Castanea sativa shells [ 45 ] or leaves and burs [ 11 ] inhibited both NF-kB activity and NO production after LPS stimulation. The suppression of the excessive NO production and iNOS expression was also reported by Kang [ 46 ] in BV-2 cells pre-treated with chestnut ( Castanea crenata ) peel extracts and stimulated with LPS. Other authors obtained the same results in LPS-stimulated microglia cells treated with isolated flavonoids also found in Castanea , such as kaempferol [ 39 ], isorhamnetin [ 38 ], or astragalin [ 47 ].…”

Section: Discussionsupporting

confidence: 71%

By-Product Extracts from Castanea sativa Counteract Hallmarks of Neuroinflammation in a Microglial Model

et al. 2023

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Castanea sativa is very common in Italy, and the large amount of waste material generated during chestnut processing has a high environmental impact. Several studies demonstrated that chestnut by-products are a good source of bioactive compounds, mainly endowed with antioxidant properties. This study further investigates the anti-neuroinflammatory effect of chestnut leaf and spiny bur extracts, together with the deepest phytochemical characterisation (by NMR and MS) of active biomolecules contained in leaf extracts, which resulted in being more effective than spiny bur ones. BV-2 microglial cells stimulated with lipopolysaccharide (LPS) were used as a model of neuroinflammation. In BV-2 cells pre-treated with chestnut extracts, LPS signalling is partially blocked via the reduced expression of TLR4 and CD14 as well as the expression of LPS-induced inflammatory markers. Leaf extract fractions revealed the presence of specific flavonoids, such as isorhamnetin glucoside, astragalin, myricitrin, kaempferol 3-rhamnosyl (1-6)(2″-trans-p-coumaroyl)hexoside, tiliroside and unsaturated fatty acids, all of which could be responsible for the observed anti-neuroinflammatory effects. Interestingly, the kaempferol derivative has been identified in chestnut for the first time. In conclusion, the exploitation of chestnut by-products is suitable for the achievement of two goals: satisfaction of consumers’ demand for new, natural bio-active compounds and valorisation of by-products.

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Section: Discussionsupporting

confidence: 71%

By-Product Extracts from Castanea sativa Counteract Hallmarks of Neuroinflammation in a Microglial Model

et al. 2023

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“…Lipopolysaccharide (LPS, L2654‐Sigma Aldrich, St. Louis, MO) was used to activate microglia cells. An effective concentration of ‘5 μg/ml’ was selected based on previous studies (Kang, 2014; Kocanci et al, 2024; Xu et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Goksu,

Kocanci,

Akinci

et al. 2024

Eur J of Neuroscience

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A common pathological hallmark of neurodegenerative disorders is neuronal cell death, accompanied by neuroinflammation and oxidative stress. The vasoactive intestinal peptide (VIP) is a pleiotropic peptide that combines neuroprotective and immunomodulatory actions. The gene therapy field shows long‐term promise for treating a wide range of neurodegenerative diseases (ND). In this study, we aimed to investigate the in vitro efficacy of transduction of microglia using lentiviral gene therapy vectors encoding VIP (LentiVIP). Additionally, we tested the protective effects of the secretome derived from LentiVIP‐infected “immortalized human” microglia HMC3 cells, and cells treated with Synthetic VIP (SynVIP), against toxin‐induced neurodegeneration. First, LentiVIP, which stably expresses VIP, was generated and purified. VIP secretion in microglial conditioned media (MG CM) for LentiVIP‐infected HMC3 microglia cells was confirmed. Microglia cells were activated with lipopolysaccharide, and groups were formed as follows: 1) Control, 2) SynVIP‐treated, or 3) LentiVIP‐transduced. These MG CM were applied on an in vitro neurodegenerative model formed by differentiated (d)‐SH‐SY5Y cells. Then, cell survival analysis and apoptotic nuclear staining, besides measurement of oxidative/inflammatory parameters in CM of cells were performed. Activated MG CM reduced survival rates of both control and toxin‐applied (d)‐SH‐SY5Y cells, whereas LentiVIP‐infected MG CM and SynVIP‐treated ones exhibited better survival rates. These findings were supported by apoptotic nuclear evaluations of (d)‐SH‐SY5Y cells, alongside oxidative/inflammatory parameters in their CM. LentiVIP seems worthy of further studies for the treatment of ND because of the potential of gene therapy to treat diseases effectively with a single injection.

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“…However, they remain a useful source of bioactive compounds [ 1 , 5 ]. The chestnut inner shell, in particular, has been used for many years in traditional medicine in Asian countries and is known for its antioxidant, antiallergic, antidiabetic, and anti-amnesic properties [ 6 , 7 , 8 , 9 ]. Several studies have demonstrated the biological activities of the Castanea genus, including antioxidant and anticancer activities [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Chestnut Shell (Castanea crenata) Cultivars Using UPLC-QTOF-MS and Their Antioxidant Capacity

Nam

Park

et al. 2022

Biomolecules

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The inner shell of the chestnut (Castanea crenata) has long been used in Asia as a medicinal herb for improving digestion and blood circulation, and treating diarrhea. However, most chestnut shells are now treated as waste materials in industrial peeling processes. In this study, we examined the metabolite variation among major cultivars of C. crenata shells using mass spectrometry. Among five representative cultivars, Okkwang, Porotan, and Ishizuuchi had higher levels of bioactive compounds, such as ellagic acid derivatives, ellagitannins, flavonoids, and gallic acid derivatives. Their antioxidant capacity was positively correlated with their chemical composition. The byproducts (whole shells) from the industrial peeling process were re-evaluated in comparison with the inner shell, a rich source of phenolic compounds. The phenolic acids and flavonoid glucoside derivatives were significantly higher in the whole shells, whereas the levels of flavonoids were higher in the inner shells. In addition, the whole shell extracts significantly reduced cellular reactive oxygen species production compared to the inner shell extracts. This study demonstrated the different biochemical benefits of different C. crenata cultivars through metabolic profiling and suggests that the whole shell could be used as a functional ingredient, as it has the highest levels of bioactive products and antioxidant effects.

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Inhibition of lipopolysaccharide-induced neuroinflammatory events in Bv-2 microglia by chestnut peel extract

Cited by 4 publications

References 18 publications

By-Product Extracts from Castanea sativa Counteract Hallmarks of Neuroinflammation in a Microglial Model

By-Product Extracts from Castanea sativa Counteract Hallmarks of Neuroinflammation in a Microglial Model

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Metabolic Profiling of Chestnut Shell (Castanea crenata) Cultivars Using UPLC-QTOF-MS and Their Antioxidant Capacity

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