Inhibition of LFA-1 Mediated T-Cell Motility by Naphthoquinones

Abstract

An in vitro T-cell migration assay has been established that can be used to study the effects of compounds on the development of T-cell polarisation with HuT-78 T lymphocytes. This assay indicates the ability of compounds tested to inhibit the inflammatory response by decreasing LFA-1-mediated T-cell motility. The effect of a series of naturally occurring quinone isolates on motility has been evaluated in this assay. Distinct differences have been observed between naphthoquinones, dihydrofuranonaphthoquinones and anthraquinones.

Isolation, Structure Elucidation, and Cytotoxic Evaluation of Furanonaphthoquinones from in Vitro Plantlets and Cultures of Streptocarpus dunnii

“…However, substitution of the C-7 hydroxyl does decrease cytotoxicity of 5 relative to 3 . We have recently demonstrated that compounds 1 − 6 also inhibit the development of T-cell polarization with HuT-78 T lymphocytes, which indicates the ability of compounds tested to inhibit the inflammatory response by decreasing LFA-1-mediated T-cell motility . Combined with the current report, this group of compounds warrants further evaluation to determine their potential for different forms of cancer.…”

“…We have recently demonstrated that compounds 1-6 also inhibit the development of T-cell polarization with HuT-78 T lymphocytes, which indicates the ability of compounds tested to inhibit the inflammatory response by decreasing LFA-1-mediated T-cell motility. 11 Combined with the current report, this group of Table 1. NMR Data for (3R)-7-Methoxy-R-dunnione ( 5) and (3R)-6-Hydroxy-7-methoxy-R-dunnione (6) compounds warrants further evaluation to determine their potential for different forms of cancer.…”

Bioactive Naphthoquinones from Higher Plants

“…Neither lawsone (14) nor lapachol (18) (up to 100 mM) were active. These NQs also inhibited T-cell motility in the human Jurkat T-cell line [309]. Cultures of S. dunnii (Lamiales) have been shown to produce the Studies in Natural Products Chemistry furanonaphthoquinones, a-dunnione (5) and dunnione (6) and hydroxylated derivatives.…”