Inhibition of invasion and induction of apoptosis by selenium in human malignant brain tumour cells in vitro

Rooprai, Harcharan K.; Kyriazis, Ioannis D.; Nuttall, Robert K.; Edwards, Dylan R.; Zicha, Daniel; Aubyn, Deborah; Davies, D.C.; Gullan, Richard; Pilkington, Geoffrey J.

doi:10.3892/ijo.30.5.1263

Cited by 22 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, many of these effects depended on the level and the chemical form of Se. The apparent cytotoxic role of 1 mg Se per kg body weight of this study agreed well with other studies where several seleno compounds (mainly sodium selenite but also SeMet, Se dioxide, and methyl seleninic acid) induced cell death in different mammalian cell lines [43][44][45][46][47][48][49]. Although the precise mechanisms of apoptosis induced by the Se compounds were not well understood [44]; it was believed that ROS may have played a crucial role in Se-decreased cell viability and Se-induced apoptosis [45].…”

Section: Discussionsupporting

confidence: 89%

RETRACTED ARTICLE: Effects of Cadmium Chloride and Sodium Selenite Alone or in Combination on the Liver of Male Sprague–Dawley Rats Assessed by Different Assays

Jabeen

Chaudhry

2011

Biol Trace Elem Res

View full text Add to dashboard Cite

This study assessed the impact of either cadmium chloride (Cd) or sodium selenite (Se) alone or in combination on male Sprague-Dawley rats. For this purpose, body and liver weights, comet and TUNEL assays, histological analysis and levels of lipid peroxidation and antioxidants in liver were determined in four groups of male Sprague-Dawley rats. The rats were given subcutaneous doses of 1 mg/kg body weight (BW) of either normal saline (control=Ct) or Cd or Se or Cd plus Se (Cd+Se) on alternate days for 4 weeks. The Cd group showed increased DNA damage, apoptosis and hepatic levels of lipid peroxidation and altered histology. Conversely, the antioxidant levels in this group were decreased as compared with the control group. The Se group also showed DNA damage, apoptosis and altered histology and reduced catalase activity, but it was less severe than the Cd group. In the Cd+Se group, ameliorating effects of Se on Cd-induced changes were observed. While the Se was able to curtail the toxic effect of Cd, the Cd or Se alone were genotoxic and cytotoxic for rats receiving a high pharmacological but non-fatal dose of 1 mg/kg BW.

show abstract

Section: Discussionsupporting

confidence: 89%

RETRACTED ARTICLE: Effects of Cadmium Chloride and Sodium Selenite Alone or in Combination on the Liver of Male Sprague–Dawley Rats Assessed by Different Assays

Jabeen

Chaudhry

2011

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…NAC was also shown to decrease the expression and activity of MMP-2 in hepatic fibrosis of bile duct ligated rats while normalizing the expression of TIMP-2 (59). Similarly, monomethyl selenium inhibited MMP-2 expression in vascular endothelial cells (60) and selenium downregulated the gene expression of MMP-2 in human malignant brain tumor cells in vitro (61). Note that the same specific nutrient synergy exhibited anti-angiogenic, anti-metastatic, and anti-invasion roles both in vitro and in vivo and was proven to be non-toxic even at very high doses (7).…”

Section: Discussionmentioning

confidence: 99%

Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes

Harakeh

Khouzam

Damanhouri

et al. 2014

International Journal of Oncology

View full text Add to dashboard Cite

Abstract.Experimental and clinical studies have revealed the effectiveness of a specific nutrient synergy (SNS) mixture composed of ascorbic acid (AA), lysine, proline, arginine, epigallocatechin gallate (EGCG) and other micronutrients in targeting crucial physiological mechanisms involved in cancer progression and metastasis. HTLV-1 causes adult T-cell leukemia (ATL). The spread and metastases of ATL as well as other tumors has been associated with matrix metalloproteinases, especially the gelatinases MMP-2 and MMP-9. The objective of this study was to investigate whether SNS, AA and EGCG affects the gelatinolytic activity of MMP-2 and its transcriptional and translational levels in HTLV-1-positive and -negative malignant T-cells. The results indicated that SNS and EGCG caused a dose-dependent decline in the activity, transcription and translation of MMP-2 after treatment with SNS and EGCG, while AA was only able to inhibit the activity at maximum doses tested and to some extent, the protein expression levels of MMP-2, without affecting their transcriptional levels. The highest activity was noted in the case of SNS which is likely to be due to a synergistic effect of the different constituents in the formulation. These results point towards the potential integration of SNS in the anti-invasive treatment of ATL and related diseases.

show abstract

“…Rooprai et al [77] demonstrated that selenite induced apoptosis in human astrocytoma cells, IPSB-18, derived from a biopsy of an anaplastic astrocytoma. Their data also suggested extensive changes in the expression of MMPs.…”

Section: Other Cancersmentioning

confidence: 99%

Is Selenium a Potential Treatment for Cancer Metastasis?

2013

View full text Add to dashboard Cite

Selenium (Se) is an essential micronutrient that functions as a redox gatekeeper through its incorporation into proteins to alleviate oxidative stress in cells. Although the epidemiological data are somewhat controversial, the results of many studies suggest that inorganic and organic forms of Se negatively affect cancer progression, and that several selenoproteins, such as GPXs, also play important roles in tumor development. Recently, a few scientists have examined the relationship between Se and metastasis, a late event in cancer progression, and have evaluated the potential of Se as an anti-angiogenesis or anti-metastasis agent. In this review, we present the current knowledge about Se compounds and selenoproteins, and their effects on the development of metastasis, with an emphasis on cell migration, invasion, and angiogenesis. In the cancers of breast, prostate, colorectal, fibrosarcoma, melanoma, liver, lung, oral squamous cell carcinoma, and brain glioma, there is either clinical evidence linking selenoproteins, such as thioredoxin reductase-1 to lymph node metastasis; in vitro studies indicating that Se compounds and selenoproteins inhibited cell motility, migration, and invasion, and reduced angiogenic factors in some of these cancer cells; or animal studies showing that Se supplementation resulted in reduced microvessel density and metastasis. Together, these data support the notion that Se may be an anti-metastastatic element in addition to being a cancer preventative agent.

show abstract

Inhibition of invasion and induction of apoptosis by selenium in human malignant brain tumour cells in vitro

Cited by 22 publications

References 0 publications

RETRACTED ARTICLE: Effects of Cadmium Chloride and Sodium Selenite Alone or in Combination on the Liver of Male Sprague–Dawley Rats Assessed by Different Assays

RETRACTED ARTICLE: Effects of Cadmium Chloride and Sodium Selenite Alone or in Combination on the Liver of Male Sprague–Dawley Rats Assessed by Different Assays

Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes

Is Selenium a Potential Treatment for Cancer Metastasis?

Contact Info

Product

Resources

About