Inhibition of insect juvenile hormone synthesis by phorbol 12‐myristate 13‐acetate

Farnsworth, Dan E.

doi:10.1016/0014-5793(87)80399-x

Cited by 18 publications

(3 citation statements)

References 15 publications

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“…However, in this study, we found that inhibition of JH biosynthesis in A. aegypti by AS-C is relieved by the addition of FA and not by MVA. A possible explanation is that AS-C partially inhibited target enzyme(s) between MVA and FA formation, although the use of exogenous mevalonate as a probe of the physiology of the CA is made difficult by the limited penetration of MVA into the gland cells (28).…”

Section: Discussionmentioning

confidence: 99%

Biochemical, Molecular, and Functional Characterization of PISCF-Allatostatin, a Regulator of Juvenile Hormone Biosynthesis in the Mosquito Aedes aegypti

Hernández‐Martínez

Fernández

et al. 2006

Journal of Biological Chemistry

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Aedes aegypti PISCF-allatostatin or allatostatin-C (Ae-AS-C) was isolated using a combination of high performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA). The matrix-assisted laser desorption/ionization timeof-flight (TOF) mass spectrum of positive ELISA fractions revealed a molecular mass of 1919.0 Da, in agreement with the sequence qIRYRQCYFNPISCF, with bridged cysteines. This sequence was confirmed by matrix-assisted laser desorption/ ionization tandem TOF/TOF mass spectrometry analysis. The corresponding Ae-AS-C cDNA was amplified by PCR, and the sequence of the peptide was confirmed. An in vitro radiochemical assay was used to study the inhibitory effect of synthetic Ae-AS-C on juvenile hormone biosynthesis by the isolated corpora allata (CA) of adult female A. aegypti. The inhibitory action of synthetic Ae-AS-C was dose-dependent; with a maximum at 10 ؊9 M. Ae-AS-C showed no inhibitory activity in the presence of farnesoic acid, an immediate precursor of juvenile hormone, indicating that the Ae-AS-C target is located before the formation of farnesoic acid in the pathway. The sensitivity of the CA to inhibition by Ae-AS-C in the in vitro assay varied during the adult life; the CA was most sensitive during periods of low synthetic activity. In addition, the levels of Ae-AS-C in the brain were studied using ELISA and reached a maximum at 3 days after eclosion. These studies suggest that Ae-AS-C is an important regulator of CA activity in A. aegypti.

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Section: Discussionmentioning

confidence: 99%

Biochemical, Molecular, and Functional Characterization of PISCF-Allatostatin, a Regulator of Juvenile Hormone Biosynthesis in the Mosquito Aedes aegypti

Hernández‐Martínez

Fernández

et al. 2006

Journal of Biological Chemistry

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“…The inhibition of JH biosynthesis by Dippu-ASTs is a complex process, probably involving more than one second messenger. Previous studies showed that ASTs regulate JH biosynthesis by acting through the inositol trisphosphate (IP 3 )/diglyceride (DAG) second messenger systems, in which protein kinase C (PKC) and Ca 2þ are involved (Feyereisen and Farnsworth, 1987b;Rachinsky and Tobe, 1996;Rachinsky et al, 1994). However, the role of Ca 2þ in regulation of JH biosynthesis is confounding.…”

Section: Thiol Hmgs Hmgrmentioning

confidence: 99%

Mode of action of allatostatins in the regulation of juvenile hormone biosynthesis in the cockroach, Diploptera punctata

Huang

Marchal

Hult

et al. 2014

Insect Biochemistry and Molecular Biology

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The FGLamide allatostatins (FGL/ASTs) are a family of neuropeptides with pleiotropic functions, including the inhibition of juvenile hormone (JH) biosynthesis, vitellogenesis and muscle contraction. In the cockroach, Diploptera punctata, thirteen FGLa/ASTs and one allatostatin receptor (AstR) have been identified. However, the mode of action of ASTs in regulation of JH biosynthesis remains unclear. Here, we determined the tissue distribution of Dippu-AstR. And we expressed Dippu-AstR in vertebrate cell lines, and activated the receptor with the Dippu-ASTs. Our results show that all thirteen ASTs activated Dippu-AstR in a dose dependent manner, albeit with different potencies. Functional analysis of AstR in multiple cell lines demonstrated that activation of the AstR receptor resulted in elevated levels of Ca(2+) and cAMP, which suggests that Dippu-AstR can act through the Gαq and Gαs protein pathways. The study on the target of AST action reveals that FGL/AST affects JH biosynthesis prior to the entry of acetyl-CoA into the JH biosynthetic pathway.

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“…Glucose could conceivably be metabolized to propionate, via lactate and acrylate, and so preferentially label JH II. However, we expected that [U-14C]glucose should efficiently label JH III (21,22). Leucine, although not a source of propionate, incorporates into terpenoid compounds including JH III (22) and thus was a useful compound to compare with the other amino acids studied.…”

Section: Resultsmentioning

confidence: 99%

Sources of propionate for the biogenesis of ethyl-branched insect juvenile hormones: Role of isoleucine and valine

Brindle

Baker

Tsai

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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Corpora allata from adult female Manduca sexta biosynthesize the sesquiterpenoid juvenile hormone (JH) III and the unusual ethyl-branched homologue JH U in vitro. We maintained corpora allata in medium 199 using [methyl-3ll1methionine as the source of the JH methyl ester moiety and as a mass marker. This allowed measurement of the relative contributions of '4C-labeled precursors to the biogenesis of JH II and III carbon skeletons. We showed efficient incorporation of a propionate equivalent, from isoleucine or valine catabolism, into the ethyl-branched portion of JH II, using doublelabel liquid scintillation counting of isolated JHs and gas chromatography/mass spectrometry with selected ion monitoring of JH deuteromethoxyhydrin derivatives. Methionine was a poor source of propionate for JH II biosynthesis, while glucose, succinate, threonine, and f8-alanine did not contribute propionate at all. Leucine, isoleucine, and glucose incorporated into JH III and the acetate-derived portion of JH II.Of the known insect juvenile hormones (JHs; Fig. 1 (6). Additional studies with corpora allata (the glands that produce JH) homogenates or whole glands demonstrated the biogenesis of important intermediates (7-10) and 4-Me JH I (11). However, no progress has been made in elucidating the regulatory processes that destine an insect to produce only JH III vs. ethyl-branched JH homologues or both. As propionyl-CoA is crucial for the biosynthesis of ethyl-branched JHs, the metabolic control of this precursor may govern the identity of the JH produced. Thus, it is important to determine which specific biochemical pathway contributes propionyl-CoA for ethyl-branched JH biosynthesis.We examined various amino acids, succinate, and glucose as possible sources of propionyl-CoA. An important consideration in the design of this study was the evidence from prior studies with Manduca sexta corpora allata (3) were added as appropriate to provide a fully reconstituted medium. To improve buffering capacity, the concentrations of KH2PO4 and Na2HPO4 were increased to 8.0 mM and 6.0 mM, respectively.Methods. M. sexta were reared as described (14) and the corpora cardiaca-corpora allata (CC-CA) complexes were obtained (15) from adult females 0-16 hr old.Sixteen CC-CA were used for each experiment. These were incubated with vigorous shaking for 1 hr at 28°C in minimal medium containing 100 ,uM L-[methyl-3Hlmethio-nine (1 Ci/mmol), four CC-CA per 100 ,u1. The preincubation depletes endogenous precursors of JH and equilibrates the CC-CA with labeled methionine that serves as mass marker for JH quantification (3). Each group offour CC-CA was then transferred into 100 p.l of fully reconstituted medium containing 100 ,uM L-[methyl-3H]methionine (1 Ci/mmol) and the 14C precursor for study at a concentration equivalent to the unlabeled compound in medium 199. We also studied radiolabeled succinate and f3-alanine (normally not present in medium 199) each at 1 mM. After 4 hr of incubation in the double-labeled medium, JHs were extracted (three tim...

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Inhibition of insect juvenile hormone synthesis by phorbol 12‐myristate 13‐acetate

Cited by 18 publications

References 15 publications

Biochemical, Molecular, and Functional Characterization of PISCF-Allatostatin, a Regulator of Juvenile Hormone Biosynthesis in the Mosquito Aedes aegypti

Biochemical, Molecular, and Functional Characterization of PISCF-Allatostatin, a Regulator of Juvenile Hormone Biosynthesis in the Mosquito Aedes aegypti

Mode of action of allatostatins in the regulation of juvenile hormone biosynthesis in the cockroach, Diploptera punctata

Sources of propionate for the biogenesis of ethyl-branched insect juvenile hormones: Role of isoleucine and valine

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