Inhibition of initiation of DNA-dependent RNA synthesis by an antibiotic B44P

Mizuno, Satoshi; Yamazaki, Hisaji; Nitta, Kazuo; Umezawa, Hamao

doi:10.1016/0006-291x(68)90755-9

Cited by 35 publications

(8 citation statements)

References 8 publications

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“…Streptovaricins (X, Figure 2) (38)(39)(40)(41) and tolypomycins belong to this group (42)(43)(44). Streptovaricins have antibiotic activities closely resembling those of rifamycins, they have the same mecha nism of action (45,46), and they show cross-resistance with rifamycins (47)(48)(49)(50). Some streptovaricins also exhibit an activity on poxviruses and on RNA-dcpendent DNA polymerase (51,52).…”

Section: Chemistrymentioning

confidence: 99%

Rifamycins: A General View

Riva¹,

Silvestri²

1972

Annu. Rev. Microbiol.

126

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Section: Chemistrymentioning

confidence: 99%

Rifamycins: A General View

Riva¹,

Silvestri²

1972

Annu. Rev. Microbiol.

126

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“…The streptovaricins and tolypomycins have biochemical properties closely resembling those of the rifamycins, as would be expected from their chemical similarity. They inhibit the initiation step of bacterial RNA polymerase (103,104), although to a considerably lesser degree than the most active rifamycins. This is due to the fact that they form a less stable complex with RNA polymerase (Wehrli et al, in preparation).…”

Section: Compounds Related To Thementioning

confidence: 99%

Actions of the rifamycins.

Wehrli¹,

Staehelin²

1971

Bacteriological Reviews

162

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“…As shown in Table 1, low concentrations of detergents (Lubrol W, Triton X-100, and Na-DOC) inhibited the synthesis of RNA by spheroplasts and disrupted spheroplasts almost completely, whereas synthesis by intact cells was not affected. The synthesis of RNA by intact cells was also less susceptible to inhibition by antibiotics which effectively inhibit DNA-dependent RNA synthesis in gram-positive bacteria (10,12,14,22,26,27). Low concentrations of actinomycin D, chromomycin As, streptovaricin, and rifampin had little effect on the synthesis of RNA by intact cells but inhibited synthesis by intact and disrupted spheroplasts.…”

Section: Resultsmentioning

confidence: 98%

Synthesis of Protein and Nucleic Acid by Disrupted Spheroplasts of Pseudomonas schuylkilliensis

et al. 1971

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Osmotically shocked spheroplasts obtained from Pseudomonas schuylkilliensis strain P contained about 54,32,28, and 82% of the total cellular protein, ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and phospholipid, respectively. This preparation was capable of incorporating 3apP-orthophosphate into RNA and DNA, aH-adenosine or 3H-uridine into RNA, and 3H-leucine or 14C-phenylalanine into protein. These activities were not found in the cytoplasmic fraction which contained most of the glucose-6-phosphate dehydrogenase activity. The synthesis of RNA by intact and disrupted spheroplast preparations was sensitive to actinomycin D, chromomycin Aa, streptovaricin, rifampin, Lubrol W, Triton X-100, and sodium deoxycholate, whereas RNA synthesis by intact cells was insensitive to these agents. Ethylenediaminetetraacetic acid, porcine pancreatic lipase, the protoplast-bursting factor, high concentrations of salts, and washing the preparation inhibited the synthesis of RNA by disrupted spheroplasts but had little or no effect on intact spheroplasts. Most of the newly synthesized RNA made by disrupted spheroplasts had the characteristics of messenger RNA. The DNA present in this preparation functioned as a template for RNA synthesis; continued protein synthesis was dependent on concomitant RNA synthesis. An unusual feature of the preparation was the finding that the synthesis of macromolecules was completely dependent on oxidative phosphorylation.

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Inhibition of initiation of DNA-dependent RNA synthesis by an antibiotic B44P

Cited by 35 publications

References 8 publications

Rifamycins: A General View

Rifamycins: A General View

Actions of the rifamycins.

Synthesis of Protein and Nucleic Acid by Disrupted Spheroplasts of Pseudomonas schuylkilliensis

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