Inhibition of Influenza A Virus Replication by Resveratrol

Abstract

We have previously shown that the life cycles of several viruses are influenced by host-cell redox states. Reports of the antioxidant activities of the plant polyphenol resveratrol (RV) prompted us to investigate its effects on influenza virus replication in vitro and in vivo. We found that RV strongly inhibited the replication of influenza virus in MDCK cells but that this activity was not directly related to glutathione-mediated antioxidant activity. Rather, it involved the blockade of the nuclear-cytoplasmic translocation of viral ribonucleoproteins and reduced expression of late viral proteins seemingly related to the inhibition of protein kinase C activity and its dependent pathways. RV also significantly improved survival and decreased pulmonary viral titers in influenza virus-infected mice. No toxic effects were observed in vitro or in vivo. That RV acts by inhibiting a cellular, rather than a viral, function suggests that it could be a particularly valuable anti-influenza drug.

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

“…Thereby, it is conceivable that the inhibitory effect of PGG was mainly caused by the direct interaction of PGG with virus. Numerous studies have demonstrated that plant polyphenols including the tea catechins (-) epigallocatechin gallate (EGCG) and theaflavin digallate [34], resveratrol (RV) [26], a polyphenol rich extract (CYSTUS052) [11], pomegranate polyphenol extract (PPE) [13] , oligonol [12], and Hydroxytyrosol (HT) [38], have potent antiviral activity against influenza virus, that are related to the nature of their interactions with viral particles. Additionally, indirect effects of some polyphenols, such as EGCG and strictinin, on host cells that might interfere with virus-cell membrane fusion have also been suggested [23,32].…”

Antioxidant Therapy as a Potential Approach to Severe Influenza-Associated Complications

“…Resveratrol inhibited the replication of influenza virus in MDCK cells, as a result of the blockade of the nuclearcytoplasmic translocation of viral ribonucleoproteins and the reduced expression of late viral protein, such as HA and matrix protein [67]. Resveratol also improved survival and decreased pulmonary viral titers in influenza virus-infected mice [67].…”

“…Resveratrol inhibited the replication of influenza virus in MDCK cells, as a result of the blockade of the nuclearcytoplasmic translocation of viral ribonucleoproteins and the reduced expression of late viral protein, such as HA and matrix protein [67]. Resveratol also improved survival and decreased pulmonary viral titers in influenza virus-infected mice [67]. Since resveratrol inhibited the induction of RANTES production by influenza virus infection in A549 lung epithelial cells [68], it is presumed that resveratorol attenuate the activation of macrophages during influenza virus infection.…”

Resveratrol: A miraculous natural compound for diseases treatment

“…Antiviral effects of RSV have been investigated in several human and animal viruses including influenza A virus (Palamara et al., 2005), Epstein‐Bar virus (Chen, Qiao et al., 2012), herpes simplex virus (Chen, Qiao et al., 2012), HIV (Clouser et al., 2012), and hepatitis C virus (Nakamura et al., 2010). Results from most of these studies indicate that RSV can prevent inhibiting protein synthesis and thus inhibit virus proliferation (Yang et al., 2015).…”