Inhibition of Inflammatory Cytokine Production and Protection Against Endotoxin Toxicity by Benzidamine

Sironi, Marina; Pozzi, Pietro; Polentarutti, Nadia; Benigni, Fabio; Coletta, Isabella; Guglielmotti, Angelo; Milanese, Claudio; Ghezzi, Pietro; Vecchi, Annunciata; Pinza, M.; Mantovani, Alberto

doi:10.1006/cyto.1996.0094

Cited by 48 publications

(43 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consequently, many attempts have been made to find anti-cytokine agents of some clinical relevance for the treatment of patients with sepsis [3,4,[11][12][13] but so far trials have been unsuccessful in reducing mortality significantly [14]. Previous findings have shown that benzydamine, a nonsteroidal anti-inflammatory agent devoid of effects on arachidonate metabolism, consistently inhibits the production of TNF in vitro and in vivo [5][6][7]. On this basis, the present study was designed to verify the potency and the therapeutic activity of this molecule in a simple animal model of endotoxin-induced shock.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Benzydamine protection in a mouse model of endotoxemia

et al. 1997

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…The drug is extensively used in clinical practice for the topical treatment of inflammatory conditions [6]. Recent studies have shown that benzydamine reduces TNF production and protects mice from LPS-induced mortality [7].…”

Section: Introductionmentioning

confidence: 99%

Benzydamine protection in a mouse model of endotoxemia

et al. 1997

Self Cite

View full text Add to dashboard Cite

show abstract

“…Total cellular RNA was prepared from hand-picked human pancreatic cultured islets. Total RNA was extracted by the guanidinium thiocyanate method, blotted, and hybridized as previously described (48). MCP-1 and CCR2B cDNAs were obtained by PCR amplification of the reported sequences (49).…”

Section: Methodsmentioning

confidence: 99%

Human Pancreatic Islets Produce and Secrete MCP-1/CCL2: Relevance in Human Islet Transplantation

et al. 2002

View full text Add to dashboard Cite

We investigated the capacity of human islets to produce monocyte chemoattractant protein-1 (MCP-1). Primary cultures of pancreatic islets expressed and secreted MCP-1, as determined by Northern blot, immunohistochemistry, in situ hybridization, and enzyme-linked immunosorbent assay. The produced MCP-1 was biologically active as it attracted monocytes in chemotaxis assay, and chemotactic activity was almost abrogated by a neutralizing anti-MCP-1 monoclonal antibody. Expression of MCP-1 was increased by primary inflammatory cytokines (interleukin-1␤

show abstract

“…described (Sironi et al, 1996). RNA transfer to membranes was checked by UV irradiation, as shown in Figure 3.…”

Section: Northern Blot Analysismentioning

confidence: 99%

Inhibition of Monocyte Chemotactic Protein-1 Synthesis by Statins

Romano

Diomede

Sironi

et al. 2000

Laboratory Investigation

277

136

View full text Add to dashboard Cite

SUMMARY:The beneficial effects of statins on the reduction of cardiovascular events has been partly attributed to their anti-inflammatory properties. In the complex of the different pathogenetic events leading to atherosclerosis, recent data suggest a central role of monocyte chemotactic protein-1 (MCP-1), because mice knock-out for MCP-1 or its receptor CC-chemokine receptor 2 were considerably resistant to plaque formation. In this study we investigated the effect of different statins on in vitro and in vivo production of MCP-1. Lovastatin and simvastatin caused a dose-dependent inhibition of MCP-1 production in peripheral blood mononuclear cells exposed to lipopolysaccharide or inactivated Streptococcus hemoliticus and in human endothelial cells exposed to interleukin-1␤. The addition of mevalonate overrode the inhibitory effect of statins indicating that mevalonate-derived products are important for chemokine production. The in vivo anti-inflammatory effect of statins was investigated using the mouse air-pouch model of local inflammation. Lovastatin and pravastatin were orally administered to mice according to a treatment schedule that significantly inhibited the hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase activity without affecting total blood cholesterol. At the dose of 10 mg/kg, lovastatin and pravastatin reduced by approximately 50% the lipopolysaccharide-induced leukocytes recruitment and the exudate MCP-1 production. In conclusion, statins, by inhibiting mevalonate-derived products, reduced both in vitro and in vivo the production of chemokines involved in leukocyte migration, and this effect is unrelated to their cholesterol-lowering action. (Lab Invest 2000, 80:1095-1100.

show abstract

Inhibition of Inflammatory Cytokine Production and Protection Against Endotoxin Toxicity by Benzidamine

Cited by 48 publications

References 0 publications

Benzydamine protection in a mouse model of endotoxemia

Benzydamine protection in a mouse model of endotoxemia

Human Pancreatic Islets Produce and Secrete MCP-1/CCL2: Relevance in Human Islet Transplantation

Inhibition of Monocyte Chemotactic Protein-1 Synthesis by Statins

Contact Info

Product

Resources

About