Inhibition of in-stent restenosis after graphene oxide double-layer drug coating with good biocompatibility

Ge, Shuang; Xi, Yadong; Du, Ruolin; Ren, Yuzhen; Xu, Zichen; Tan, Youhua; Wang, Shaobin; Yin, Tieying; Wang, Guixue

doi:10.1093/rb/rbz010

Cited by 26 publications

(20 citation statements)

References 56 publications

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“…Importantly, the prepared materials do not break down erythrocytes. Hemolysis tests confirmed the results obtained by S. Ge et al [ 17 ]. The coatings with an inner layer of GO loaded with docetaxel and an outer layer of carboxymethyl loaded with heparin, tested by that research group, did not cause hemolysis higher than 5%, which is following the guidelines.…”

Section: Discussionsupporting

confidence: 90%

“…GO can also be used to modify other surfaces, e.g., polymer surfaces, in the production of scaffolds [ 14 , 15 ]. Other studies show that graphene oxide as an intermediate layer in coatings, in combination with heparin and poly(3,4-ethylenedioxythiophene) [ 16 ] or drugs, i.e., docetaxel [ 17 ], may improve the biocompatibility of steel stents and reduce platelet adhesion. Still, the status of graphene oxide as a biomaterial remains unsettled.…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical and Biological Properties of Graphene-Oxide-Coated Metallic Materials

et al. 2021

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In this article, we present graphene oxide (produced by a modified Hummers’ method) coatings obtained using two different methods: electrophoretic deposition on 316L stainless steel and chemical modification of the surface of gold applied to the steel. The coating properties were characterized by microscopic and spectrometric techniques. The contact angle was also determined, ranging from 50° to 70°. Our results indicated that GO coatings on steel and gold were not toxic towards L929 cells in a direct cell adhesion test—on all tested materials, it was possible to observe the growth of L929 cells during 48 h of culture. The lack of toxic effect on cells was also confirmed in two viability tests, XTT and MTT. For most of the tested materials, the cell viability was above 70%. They showed that the stability of the coating is the crucial factor for such GO coatings, and prove that GO in the form of coating is non-toxic; however, it can show toxicity if detached from the surface. The obtained materials also did not show any hemolytic properties, as the percentage of hemolysis was on the level of the negative control, which is very promising in the light of future potential applications.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Physicochemical and Biological Properties of Graphene-Oxide-Coated Metallic Materials

et al. 2021

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“…This reduction in platelet surface coverage was far greater than the amount of reduction observed for recent heparin drug-eluting stents [ 23 , 24 , 26 ], which showed far more platelet adhesion to their modified steel samples as compared to MEG-OH-coated samples, and poorer reduction in adhesion of less than 80% compared to the greater-than-90% adhesion observed for MEG-OH. Additionally, the amount of platelet reduction was greater than that observed for recent graphene surface coatings on steel which display an 80–90% reduction [ 30 , 31 , 32 ], and performed similarly to hydrogel coatings, with a greater-than-90% reduction [ 33 , 34 ]. As such, MEG-OH performs as well or better than the so-called state-of-the-art alternative coatings as it pertains to the prevention of thrombus formation.…”

Section: Resultsmentioning

confidence: 96%

“…Graphene-coated stents have also been investigated for their anti-thrombogenic properties on stainless steel [ 30 , 31 , 32 ]. These coatings may show greater reduction in platelet adhesion compared to heparin-eluting stents, with some reducing adhesion by 80–90%, but these also show slightly elevated cytotoxicity compared to bare stents.…”

Section: Introductionmentioning

confidence: 99%

Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel

2021

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Implantable devices fabricated from austenitic type 316L stainless steel have been employed significantly in medicine, principally because the material displays excellent mechanical characteristics and corrosion resistance. It is well known, however, that interaction of exposure of such a material to blood can initiate platelet adhesion and blood coagulation, leading to a harmful medical condition. In order to prevent undesirable surface platelet adhesion on biomaterials employed in procedures such as renal dialysis, we developed an ultrathin anti-thrombogenic covalently attached monolayer based on monoethylene glycol silane chemistry. This functions by forming an interstitial hydration layer which displays restricted mobility in the prevention of surface fouling. In the present work, the promising anti-thrombogenic properties of this film are examined with respect to platelet aggregation on 316L austenitic stainless steel exposed to whole human blood. Prior to exposure with blood, all major surface modification steps were examined by X-ray photoelectron spectroscopic analysis and surface free-angle measurement by contact angle goniometry. End-stage anti-thrombogenicity detection after 20 min of blood exposure at 100 s−1, 300 s−1, 600 s−1, 750 s−1, and 900 s−1 shear rates revealed that a significant reduction (>90%) of platelet adhesion and aggregation was achieved for surface-modified steel, compared with untreated material. This result is confirmed by experiments conducted in real time for 60-minute exposure to blood at 100 s−1, 600 s−1, and 900 s−1 shear rates.

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“…Graphene oxide (GO), a highly oxidized form of graphene, in which the quasi-two-dimensional (2D) spatial structure of carbon atoms is decorated with oxygen-containing functionalities [ 11 ]. Numerous oxygen-functional groups, including carboxylic, epoxy and hydroxyl groups make its surface open for covalent, electrostatic and hydrogen bonding with biomolecules and therapeutic drug, which endows drugs with targeted and sustained features, and improves the biocompatibility of GO [ 12 ]. Studies have reported that GO has the potential to promote cellular proliferation, differentiation and adhesion with no or little cytotoxic effect [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model

Qian

Guo

et al. 2021

Regenerative Biomaterials

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Associated with persistent oxidative stress, altered inflammatory responses, poor angiogenesis and epithelization, wound healing in diabetic patients is impaired. N-acetylcysteine (NAC) is reported to resist excess reactive oxygen species (ROS) production, prompt angiogenesis and maturation of the epidermis. Studies have revealed that graphene oxide (GO) can regulate cellular behavior and form cross-links with naturally biodegradable polymers such as collagen (COL) to construct composite scaffolds. Here, we reported a COL-based implantable scaffold containing a mixture of GO capable of the sustained delivery of NAC to evaluate the wound healing in diabetic rats. The morphological, physical characteristics, biocompatibility and NAC release profile of the GO-COL-NAC (GCN) scaffold were evaluated in vitro. Wound healing studies were performed on a 20 mm dorsal full-skin defect of streptozotocin (STZ)-induced diabetic rats. The injured skin tissue was removed at the 18th day post-surgery for histological analysis and determination of glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activity. In diabetic rats, we confirmed that the GCN scaffold presented a beneficial effect in enhancing the wound healing process. Additionally, due to the sustained release of NAC, the scaffold may potentially induce the antioxidant defense system, upregulating the expression levels of the antioxidant enzymes in the wound tissue. The findings revealed that the antioxidant biocompatible composite collagen dressing could not only deliver NAC in situ for ROS inhibition but also promote the wound healing process. This scaffold with valuable therapy potential might enrich the approaches for surgeon in diabetic wound treatment in the future.

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Inhibition of in-stent restenosis after graphene oxide double-layer drug coating with good biocompatibility

Cited by 26 publications

References 56 publications

Physicochemical and Biological Properties of Graphene-Oxide-Coated Metallic Materials

Physicochemical and Biological Properties of Graphene-Oxide-Coated Metallic Materials

Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel

Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model

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