Inhibition of
            <i>Staphylococcus epidermidis</i>
            Biofilm Formation by Rabbit Polyclonal Antibodies against the SesC Protein

Shahrooei, Mohammad; Hira, Vishal; Stijlemans, Benoı̂t; Merckx, Rita; Hermans, Peter W. M.; Eldere, Johan Van

doi:10.1128/iai.01464-08

Cited by 60 publications

(64 citation statements)

References 38 publications

(53 reference statements)

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“…The fluorescent reporter was then used to compare PhnD antibodies to those for other targets, including several proteins with a known role in S. epidermidis biofilm formation: EmbP (SERP1011), Aap (SERP2398), and SesC (SERP2264) (17)(18)(19)(20). Treatment with EmbP antibodies also led to a large decrease in biofilm formation (91% reduction versus the control); however, none of the other antibodies showed substantial effects at the endpoint (Fig.…”

Section: Resultsmentioning

confidence: 99%

Antibodies to PhnD Inhibit Staphylococcal Biofilms

et al. 2014

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Biofilm formation on central lines or peripheral catheters is a serious threat to patient well-being. Contaminated vascular devices can act as a nidus for bloodstream infection and systemic pathogen dissemination. Staphylococcal biofilms are the most common cause of central-line-associated bloodstream infections, and antibiotic resistance makes them difficult to treat. As an alternative to antibiotic intervention, we sought to identify anti-staphylococcal biofilm targets for the development of a vaccine or antibody prophylactic. A screening strategy was devised using a microfluidic system to test antibody-mediated biofilm inhibition under biologically relevant conditions of shear flow. Affinity-purified polyclonal antibodies to target antigen PhnD inhibited both Staphylococcus epidermidis and S. aureus biofilms. PhnD-specific antibodies blocked biofilm development at the initial attachment and aggregation stages, and deletion of phnD inhibited normal biofilm formation. We further adapted our microfluidic biofilm system to monitor the interaction of human neutrophils with staphylococcal biofilms and demonstrated that PhnD-specific antibodies also serve as opsonins to enhance neutrophil binding, motility, and biofilm engulfment. These data support the identification of PhnD as a lead target for biofilm intervention strategies performed either by vaccination or through passive administration of antibodies.

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Section: Resultsmentioning

confidence: 99%

Antibodies to PhnD Inhibit Staphylococcal Biofilms

et al. 2014

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“…Previous investigators have utilized the indwelling catheter model in either mice or rats for investigation of genes important for biofilm formation; [31][32][33] however, it is believed that this is the first description of active vaccination with evaluation of efficacy against early biofilm formation, in this model. A relatively early time point of 24 h was chosen for efficacy evaluation, due to the kinetics of bacterial spread and/or lethality in this model.…”

Section: Methodsmentioning

confidence: 99%

Development of a rat central venous catheter model for evaluation of vaccines to preventStaphylococcus epidermidisandStaphylococcus aureusearly biofilms

Ebert¹,

Smith²,

Pancari³

et al. 2011

Human Vaccines

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“…Pure plasmid DNA was isolated using a High Pure plasmid isolation kit (Roche Diagnostics), and PCR amplification and sequencing were used for verification. The recombinant plasmids were used for recombinant protein production as previously described in detail (28). Preparation and purification of polyclonal anti-rSes IgG antibodies.…”

Section: Methodsmentioning

confidence: 99%

Vaccination with SesC Decreases Staphylococcus epidermidis Biofilm Formation

et al. 2012

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ABSTRACTThe increased use of medical implants has resulted in a concomitant rise in device-related infections. The majority of these infections are caused byStaphylococcus epidermidisbiofilms. Immunoprophylaxis and immunotherapy targetingin vivo-expressed, biofilm-associated, bacterial cell surface-exposed proteins are promising new approaches to prevent and treat biofilm-related infections, respectively. Using anin silicoprocedure, we identified 64 proteins that are predicted to beS.epidermidissurface exposed (Ses), of which 36 were annotated as (conserved) hypothetical. Of these 36 proteins, 5 proteins—3 LPXTG motif-containing proteins (SesL, SesB, and SesC) and 2 of the largest ABC transporters (SesK and SesM)—were selected for evaluation as vaccine candidates. This choice was based on protein size, number of antigenic determinants, or the established role inS. epidermidisbiofilm formation of the protein family to which the candidate protein belongs. Anti-SesC antibodies exhibited the greatest inhibitory effect onS. epidermidisbiofilm formationin vitroand on colonization and infection in a mouse jugular vein catheter infection model that includes biofilms and organ infections. Active vaccination with a recombinant truncated SesC inhibitedS. epidermidisbiofilm formation in a rat model of subcutaneous foreign body infection. Antibodies to SesC were shown to be opsonic by anin vitroopsonophagocytosis assay. We conclude that SesC is a promising target for antibody mediated strategies againstS. epidermidisbiofilm formation.

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Inhibition of Staphylococcus epidermidis Biofilm Formation by Rabbit Polyclonal Antibodies against the SesC Protein

Cited by 60 publications

References 38 publications

Antibodies to PhnD Inhibit Staphylococcal Biofilms

Antibodies to PhnD Inhibit Staphylococcal Biofilms

Development of a rat central venous catheter model for evaluation of vaccines to preventStaphylococcus epidermidisandStaphylococcus aureusearly biofilms

Vaccination with SesC Decreases Staphylococcus epidermidis Biofilm Formation

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