Inhibition of hydroxyapatite dissolution by whole casein: the effects of pH, protein concentration, calcium, and ionic strength

Barbour, Michele E; Shellis, R.P.; Parker, DM; Allen, GC; Addy, M.

doi:10.1111/j.1600-0722.2008.00565.x

Cited by 74 publications

(66 citation statements)

References 34 publications

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“…Under acidic conditions, milk proteins show a poor affinity to tooth surfaces as both casein micelles and hydroxyapatite surfaces are supposed to be positively charged at pH below 5, thus reducing their electrostatic interactions. 12,25 Precipitates observed after application of the CPP-ACP pastes were shown to scatter over the surface resulting in a nonhomogenous layer, 8,12 which is probably dissolved by acid contact. Additionally, the surface precipitates might be mechanically removed by toothbrushing.…”

Section: Discussionmentioning

confidence: 99%

Randomised in situ trial on the effect of milk and CPP-ACP on dental erosion

Wiegand

Attin

2014

Journal of Dentistry

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OBJECTIVES This randomised in situ study aimed to analyse the effect of milk (with or without 5ppm F) and CPP-ACP pastes (with or without 900ppm F) on dental erosion. METHODS The study was a seven phase (5 days each) crossover design involving 15 participants wearing intraoral appliances with enamel and dentine specimens. Specimens were extraorally eroded (erosive soft drink, 6×90s/day) and brushed (2×30s/day, 2N) using a non-fluoridated toothpaste (negative control). The test products were milk, milk+5ppm F (twice daily, each 100ml/2min), CPP-ACP paste, CPP-ACP paste+900ppm F (3min/day) or a SnCl2/AmF/NaF mouthrinse (positive control, 30s/day), which were applied immediately after erosion with the appliances in the oral cavity. In an additional group, a fluoridated toothpaste was used without any additional test product. Tissue loss was determined profilometrically after 5 days and statistically analysed by linear mixed models methodologies (p<0.05). RESULTS Compared with the negative control (non-fluoridated toothpaste only, enamel: 2.2±1.3m; dentine: 3.8±2.2m), enamel and dentine loss was significantly reduced by the use of fluoridated toothpaste (enamel: 1.1±1.0m; dentine: 2.4±1.7m) and the SnCl2/AmF/NaF mouthrinse (1.5±1.5m; dentine: 1.8±1.9m). CON-CLUSIONS Milk and CPP-ACP were not effective in reducing enamel and dentine loss significantly, independently of the presence of fluoride. CLINICAL SIGNIFICANCE Enamel and dentine erosion were significantly reduced by the use of a fluoridated toothpaste or a SnCl2/AmF/NaF mouthrinse, but not by milk or CPP-ACP under the conditions of the present study. CLINICAL TRIALS REGISTRATION NCT01566357. Randomized in situ trial on the effect of milk and CPP-ACP on dental erosion AbstractObjectives: This randomized in situ study aimed to analyse the effect of milk (with or without 5 ppm F) and CPP-ACP pastes (with or without 900 ppm F) on dental erosion.Methods: The study was a seven phase (5 days each) crossover design involving 15 participants wearing intraoral appliances with enamel and dentin specimens. Specimens were extraorally eroded (erosive soft drink, 6 x 90s/day) and brushed (2 x 30 s/day, 2 N) using a non-fluoridated toothpaste (negative control). The test products were milk, milk + 5 ppm F (twice daily, each 100 ml/2 min), CPP-ACP paste, CPP-ACP paste + 900 ppm F (3 min/day) or a SnCl 2 /AmF/NaF mouthrinse (positive control, 30 s/day), which were applied immediately after erosion with the appliances in the oral cavity. In an additional group, a fluoridated toothpaste was used without any additional test product. Tissue loss was determined profilometrically after 5 days and statistically analysed by linear mixed models methodologies (p < 0.05).Results: Compared with the negative control (non-fluoridated toothpaste only, enamel:2.2±1.3 µm; dentin: 3.8±2.2 µm), enamel and dentin loss was significantly reduced by the use of fluoridated toothpaste (enamel: 1.1±1.0 µm; dentin: 2.4±1.7 µm) and the SnCl 2 /AmF/NaF mouthrinse (1.5±1.5 µm; dentin: 1.8±1.9 µm)....

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Section: Discussionmentioning

confidence: 99%

Randomised in situ trial on the effect of milk and CPP-ACP on dental erosion

Wiegand

Attin

2014

Journal of Dentistry

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“…Milk can act as a remineralizing agent, acting as a donor of calcium and phosphate, acting together with saliva to remineralize, but it can also promote a deposit of mineral and organic material on the enamel surface, forming a protective film [9]. This film is related to the adsorption of this casein in the enamel surface [10], thereby reducing the rate of dissolution of hydroxyapatite crystals and their stabilization by inhibiting the release of ions [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Mixture of Coffee or Chocolate to Milk in the Progression of Des-Remineralization of Tooth Enamel - An in Vitro Study

Lachowski¹,

Ferreira²,

Oliveira³

et al. 2014

Pesqui. Bras. Odontopediatria Clín. Integr.

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Objective: To evaluate if there is any interference when adding coffee or chocolate to cow milk in the enamel des-remineralization process (orange juice/saliva). Material and Methods: 48 specimens of human enamel (4x4 mm) were included in acrylic resin, ground flat, polished and randomly divided into the following experimental groups (n = 8): G1-saliva, G2-orange juice, G3 orange juice / milk; G4-Orange Juice / Chocolate, G5-Orange Juice / Coffee + milk and G6-milk. Each group was immersed for 60 seconds on each solution proposed and then immersed for 30 minutes in saliva. This cycle was repeated 4 times. Prior to these cycles, the Knoop microhardness average of each specimen was obtained. After the challenges proposed, the final microhardness average was calculated. The values obtained from the difference between the initial and final microhardness were subjected to ANOVA followed by Tukey test (p <0,05). Results: The orange juice had the highest change in microhardness and statistically different from all other groups. The microhardness change was statistically similar in the groups submitted to orange juice followed by immersion in milk, in chocolate and in the mixture milk + coffee. The pure milk and saliva caused no change in surface hardness of enamel. Conclusion: Milk or the addition of chocolate and coffee to milk was able to produce a protective effect of the enamel surface against an erosive challenge.

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“…Furthermore, other components in milk such as phosphoprotein/phosphopeptide-stabilized calcium phosphates possess re-mineralizing potential by providing calcium and phosphate (32). Casein in milk may reduce hydroxyapatite dissolution by binding to the hydroxyapatite surface and forming a thin layer; this thin layer was proposed to function as a diffusion barrier, which restricted H + access to the tooth surface and prevented further de-mineralization of enamel (33).…”

Section: Discussionmentioning

confidence: 99%

Effects of tannin-fluoride and milk-fluoride mixture on human enamel erosion from inappropriately chlorinated pool water

2017

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This in vitro study aimed to investigate the efficacy of tannin-fluoride and milk-fluoride mixtures on human enamel erosion after exposure to inappropriately chlorinated pool water. Enamel specimens were immersed in swimming pool water (pH 2.7) for 30 min and in each test reagent for 4 min once a day for 60 consecutive days (group I: control, group II: tannin-fluoride, group III: milk-fluoride, group IV: tannin-fluoride before and milk-fluoride after erosive challenge, and group V: milk containing tanninfluoride before and after erosive exposure). Surface microhardness was assessed on days 0, 30, and 60. Scanning electron microscopy (SEM) and electron probe microanalysis (EPMA) were performed after treatment of samples for 60 days. Surface microhardness of experimental groups was ranked as follows: group III > group IV-group V > group II > group I (P < 0.05). Moreover, SEM images revealed deposition of substances on erosive enamel surface after treatment with tannin-fluoride and milk-fluoride mixtures. Furthermore, EPMA profiles showed decrease of phosphorus and increase of fluoride content in groups II and IV. In conclusion, we demonstrated that treatment with fluoridated milk with or without tannin-fluoride has protective effects against enamel erosion caused by low-pH swimming pool water.

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Inhibition of hydroxyapatite dissolution by whole casein: the effects of pH, protein concentration, calcium, and ionic strength

Cited by 74 publications

References 34 publications

Randomised in situ trial on the effect of milk and CPP-ACP on dental erosion

Randomised in situ trial on the effect of milk and CPP-ACP on dental erosion

Effect of the Mixture of Coffee or Chocolate to Milk in the Progression of Des-Remineralization of Tooth Enamel - An in Vitro Study

Effects of tannin-fluoride and milk-fluoride mixture on human enamel erosion from inappropriately chlorinated pool water

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