OBJECTIVES This randomised in situ study aimed to analyse the effect of milk (with or without 5ppm F) and CPP-ACP pastes (with or without 900ppm F) on dental erosion. METHODS The study was a seven phase (5 days each) crossover design involving 15 participants wearing intraoral appliances with enamel and dentine specimens. Specimens were extraorally eroded (erosive soft drink, 6×90s/day) and brushed (2×30s/day, 2N) using a non-fluoridated toothpaste (negative control). The test products were milk, milk+5ppm F (twice daily, each 100ml/2min), CPP-ACP paste, CPP-ACP paste+900ppm F (3min/day) or a SnCl2/AmF/NaF mouthrinse (positive control, 30s/day), which were applied immediately after erosion with the appliances in the oral cavity. In an additional group, a fluoridated toothpaste was used without any additional test product. Tissue loss was determined profilometrically after 5 days and statistically analysed by linear mixed models methodologies (p<0.05). RESULTS Compared with the negative control (non-fluoridated toothpaste only, enamel: 2.2±1.3m; dentine: 3.8±2.2m), enamel and dentine loss was significantly reduced by the use of fluoridated toothpaste (enamel: 1.1±1.0m; dentine: 2.4±1.7m) and the SnCl2/AmF/NaF mouthrinse (1.5±1.5m; dentine: 1.8±1.9m). CON-CLUSIONS Milk and CPP-ACP were not effective in reducing enamel and dentine loss significantly, independently of the presence of fluoride. CLINICAL SIGNIFICANCE Enamel and dentine erosion were significantly reduced by the use of a fluoridated toothpaste or a SnCl2/AmF/NaF mouthrinse, but not by milk or CPP-ACP under the conditions of the present study. CLINICAL TRIALS REGISTRATION NCT01566357.
Randomized in situ trial on the effect of milk and CPP-ACP on dental erosion AbstractObjectives: This randomized in situ study aimed to analyse the effect of milk (with or without 5 ppm F) and CPP-ACP pastes (with or without 900 ppm F) on dental erosion.Methods: The study was a seven phase (5 days each) crossover design involving 15 participants wearing intraoral appliances with enamel and dentin specimens. Specimens were extraorally eroded (erosive soft drink, 6 x 90s/day) and brushed (2 x 30 s/day, 2 N) using a non-fluoridated toothpaste (negative control). The test products were milk, milk + 5 ppm F (twice daily, each 100 ml/2 min), CPP-ACP paste, CPP-ACP paste + 900 ppm F (3 min/day) or a SnCl 2 /AmF/NaF mouthrinse (positive control, 30 s/day), which were applied immediately after erosion with the appliances in the oral cavity. In an additional group, a fluoridated toothpaste was used without any additional test product. Tissue loss was determined profilometrically after 5 days and statistically analysed by linear mixed models methodologies (p < 0.05).Results: Compared with the negative control (non-fluoridated toothpaste only, enamel:2.2±1.3 µm; dentin: 3.8±2.2 µm), enamel and dentin loss was significantly reduced by the use of fluoridated toothpaste (enamel: 1.1±1.0 µm; dentin: 2.4±1.7 µm) and the SnCl 2 /AmF/NaF mouthrinse (1.5±1.5 µm; dentin: 1.8±1.9 µm)....