Inhibition of human pulpal gelatinases (MMP‐2 and MMP‐9) by zinc oxide cements

Santos, Maria Cristina Leme Godoy dos; Souza, Ana Paula de; Gerlach, Raquel Fernanda; Trevilatto, Paula Cristina; Scarel‐Caminaga, Raquel Mantuaneli; Line, Sérgio Roberto Peres

doi:10.1111/j.1365-2842.2004.01297.x

Cited by 49 publications

(34 citation statements)

References 31 publications

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“…26 In addition, Santos and colleagues found that ZnO 2 suppresses the production of MMP-9. 27,28 These reports, together with the results of our present study, suggest that Zn in Zn-HA partially suppresses MMP-9 production. Since the effect of Zn-HA is greater than that of ZnCl 2 alone, the MMP-9 inhibitory effect of Zn-HA is additive as compared to that of Zn alone.…”

Section: Dovepresssupporting

confidence: 82%

Inhibitory effects of zinc hyaluronate on synoviocyte growth and matrix metalloproteinase-9 activity

Saito¹

2009

JIR

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Sodium hyaluronate (Na-HA) is a therapeutic agent used for treating knee arthritis. However, it has been unsuccessfully used in the treatment of knee effusions. Joint effusion results from synovial activation and growth, leading to an increase in the production of matrix metalloproteinases (MMPs), especially MMP-9. This study aimed to determine whether the newly developed zinc hyaluronate (Zn-HA) is more effective than Na-HA in inhibiting the growth of synoviocytes or production and activity of MMPs in rheumatoid synoviocytes. Our results showed that Zn-HA inhibited synoviocyte growth, MMP-9 protein production, and MMP-9 mRNA expression, whereas Na-HA exerted only a slight inhibitory effect on these parameters. Moreover, Zn-HA induced synoviocyte apoptosis, whereas Na-HA did not. These results suggest that Zn-HA retards synoviocyte growth by inducing apoptosis following a decrease in the production of MMP-9 and mRNA. Therefore, it is suggested that Zn-HA can suppress arthritis more efficiently than Na-HA.

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Section: Dovepresssupporting

confidence: 82%

Inhibitory effects of zinc hyaluronate on synoviocyte growth and matrix metalloproteinase-9 activity

Saito¹

2009

JIR

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“…It may be concluded that zinc at high concentrations serves as a potent, stable, and effective inhibitor of collagen degradation mediated by dentine MMPs [Souza et al, 2000;Santos et al, 2004;Osorio et al, 2011c;Henn et al, 2012]. This inhibiting effect is only reversed in the presence of Ca-EDTA.…”

Section: Discussionmentioning

confidence: 98%

“…Some studies have shown inhibition of MMPs by metals, metal salts, and especially zinc present in dental products [Souza et al, 2000;Santos et al, 2004;Kato et al, 2010;Henn et al, 2012]. Zinc concentration strongly reduced MMP-mediated collagen degradation in PAetched dentine.…”

mentioning

confidence: 99%

Zinc-Inhibited MMP-Mediated Collagen Degradation after Different Dentine Demineralization Procedures

et al. 2012

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Background: Dentine matrix metalloproteinases (MMPs) play an important role in the dentine caries process. Aims: To determine if MMP-mediated collagen degradation of acid-demineralized dentine may be inhibited by zinc or zinc chelators. Methods: Human dentine specimens were demineralized by phosphoric acid (PA), ethylenediaminetetraacetic acid (EDTA), Clearfil SE Bond primer (SE), or Xeno V (XE) and stored in artificial saliva. Chlorhexidine digluconate (CHX), doxycycline, EDTA, or ZnCl₂ was added. C-terminal telopeptide determinations (ICTP) were performed by radioimmunoassay after 24 h and 4 weeks. Results: Collagen degradation was prominent in PA-demineralized (ICTP values from 74.01 µg/l at 24 h to 202.46 µg/l after 4 weeks) and EDTA-demineralized dentine (ICTP values from 83.93 µg/l at 24 h to 158.82 µg/l after 4 weeks) stored in artificial saliva. Doxycycline fully blocked proteolysis. CHX and EDTA reduced collagen degradation only at 24 h. Zinc in excess strongly inhibited hydrolysis of collagen in all tested groups (ICTP values were: PA, 13.56 µg/l; EDTA, 11.21 µg/l; SE, 1.52 µg/l, and XE, 2.37 µg/l) and its effect was maintained for up to 4 weeks, except for EDTA-treated dentine (ICTP values were: PA, 40.76 µg/l; EDTA, 79.15 µg/l; SE, 5.29 µg/l, and XE, 6.38 µg/l). Conclusion: EDTA and CHX exerted time-limited MMP inhibition, and excess zinc served as an effective inhibitor of MMP-mediated collagen degradation in strong or mildly demineralized dentine. MMP degradation of collagen was reduced in resin-infiltrated dentine; the presence of excess zinc chloride exerted an additional protective effect.

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“…MMP-9 may be involved in degradation and reconstruction of the extracellular matrix under the influence of metallic zinc ions: it could, for example, cut off extracellular matrix components, regulate cell adhesion, act on extracellular components or other protein components and activate potential biological function, and participate directly or indirectly in tissue remodelling and wound repair. 28 When MMP-9 levels are elevated, the above-mentioned functions are enhanced, which would result in excessive extracellular matrix degradation, increased tissue remodelling and myocardial fibrosis, and could contribute to micro re-entry and eventually lead to AF. 29 Our study also found that MMP-9 levels gradually increased from paroxysmal AF through persistent AF to permanent AF.…”

Section: Discussionmentioning

confidence: 99%

Changes in matrix metalloproteinase-9 levels during progression of atrial fibrillation

Yang²,

Xie³

et al. 2013

J Int Med Res

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Objectives: To observe levels of matrix metalloproteinase (MMP)-9 and evaluate their significance in various stages of idiopathic atrial fibrillation (AF). Methods: Patients with idiopathic AF were recruited into this prospective study and classified into one of three groups according to stage of disease progression: paroxysmal AF; persistent AF; permanent AF. Healthy individuals were enrolled as control subjects. Serum levels of MMP-9 in all four groups were determined using a double-antibody sandwich enzyme-linked immunosorbent assay. Results: Each AF group included 25 patients; 40 healthy individuals were included as controls. MMP-9 levels in the three AF groups were significantly higher than in the control group: 168.72 AE 25.970, 201.36 AE 31.26 and 253.20 AE 22.99 ng/ml for the paroxysmal, persistent and permanent AF groups respectively, versus 76.80 AE 14.90 ng/ml for the control group. MMP-9 levels increased with idiopathic AF disease progression (P < 0.05). Conclusions: An elevated MMP-9 level appears to be associated with a diagnosis of AF. MMP-9 levels appear to increase in relation to the stage of idiopathic AF progression.

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Inhibition of human pulpal gelatinases (MMP‐2 and MMP‐9) by zinc oxide cements

Cited by 49 publications

References 31 publications

Inhibitory effects of zinc hyaluronate on synoviocyte growth and matrix metalloproteinase-9 activity

Inhibitory effects of zinc hyaluronate on synoviocyte growth and matrix metalloproteinase-9 activity

Zinc-Inhibited MMP-Mediated Collagen Degradation after Different Dentine Demineralization Procedures

Changes in matrix metalloproteinase-9 levels during progression of atrial fibrillation

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