Inhibition of human ovarian cancer cell proliferation in vitro by neuroendocrine hormones

Kikuchi, Yoshihiro; Kita, Toru; Miyauchi, Munenori; Iwano, Ichiro; Kato, Koichi

doi:10.1016/0090-8258(89)90851-2

Cited by 24 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nonetheless, anticancer actions of melatonin against ovarian cancer are inconsistent. Kikuchi et al [43] previously reported that melatonin had no inhibitory effect on the proliferation of ovarian cancer cells compared with other neuroendocrine hormones. In contrast, Bartsch et al [44] observed that melatonin was an inhibitor of ovarian carcinoma in individual cases of patients.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Melatonin synergistically enhances cisplatin‐induced apoptosis via the dephosphorylation of ERK/p90 ribosomal S6 kinase/heat shock protein 27 in SK‐OV‐3 cells

Kim¹,

Jeong²,

Kim

et al. 2011

Journal of Pineal Research

View full text Add to dashboard Cite

To evaluate melatonin's ability to enhance ovarian cancer cells to cisplatin treatment for ovarian cancer, this study was performed. Melatonin by itself had no significant cytotoxicity against SK-OV-3 cells, while cisplatin suppressed the cell viability in a dose-dependent manner. Combined treatment with cisplatin and melatonin synergistically inhibited the viability of SK-OV-3 cells with the synergism between two drugs (1 > combination index). In contrast, melatonin revealed the protective effect against cisplatin-induced cytotoxicity in OSEN normal ovarian epithelial cells. Cotreatment with cisplatin and melatonin increased the sub-G1 DNA contents and TdT-mediated dUTP nick end-labeling (TUNEL)-positive cells compared with cisplatin control in SK-OV-3 cells, suggesting that melatonin augments cisplatin-induced apoptosis. Consistently, combined treatment of cisplatin and melatonin increased the cleavage of caspase-3 and poly-(ADP-ribose) polymerase (PARP). Importantly, melatonin synergistically inhibited the phosphorylation of extracellular signal-regulated kinase (ERK) along with dephosphorylation of 90-kDa ribosomal S6 kinase (p90RSK) and heat shock protein 27 (HSP27) induced by cisplatin. Furthermore, melatonin remarkably blocked the expression and colocalization of p90RSK and HSP27 by combination treatment with cisplatin. Taken together, our findings demonstrate that melatonin enhances cisplatin-induced apoptosis via the inactivation of ERK/p90RSK/HSP27 cascade in SK-OV-3 cells as a potent synergist to cisplatin treatment.

show abstract

Section: Discussionmentioning

confidence: 98%

“…Kikuchi et al. [43] previously reported that melatonin had no inhibitory effect on the proliferation of ovarian cancer cells compared with other neuroendocrine hormones. In contrast, Bartsch et al.…”

Section: Discussionmentioning

confidence: 99%

Melatonin synergistically enhances cisplatin‐induced apoptosis via the dephosphorylation of ERK/p90 ribosomal S6 kinase/heat shock protein 27 in SK‐OV‐3 cells

Kim¹,

Jeong²,

Kim

et al. 2011

Journal of Pineal Research

View full text Add to dashboard Cite

show abstract

“…We have also demonstrated that the KF cell proliferation was dose-dependently inhibited by endorphins and to less extent [Met]enkephalin [7]. Thus, in the present study we attempted to determine effects of opioid peptides on natutal or KF-cell-specific cytotoxicity in spleen cells of intact or tumor-bearing nude mice.…”

Section: Offprint Requests To: Y Kikuchimentioning

confidence: 82%

Effects of opioid peptides on the cellular immunity in spleen cells from intact nude mice or nude mice bearing human ovarian carcinoma

Kikuchi

Kita

Nagata

1990

Cancer Immunol Immunother

Self Cite

View full text Add to dashboard Cite

The present study was designed to explore the effects of opioid peptides on the lytic activity of spleen cells from intact nude mice or nude mice bearing human ovarian cancer cells (KF). When the spleen cells from intact nude mice were incubated with various concentrations of opioid peptides, the ability of the spleen cells to lyse the KF cells was significantly stimulated between 0.05 nM and 50 nM concentrations of all opioid peptides used in this study. The degree of stimulation was most marked at 5 nM opioid peptides and the most marked stimulatory effect was obtained by alpha-endorphin. On the other hand, the lytic activity of spleen cells from nude mice challenged with the KF cells was about two-fold higher than that of intact nude mice, suggesting that spleen cells from nude mice challenged with KF cells have KF-cell-specific cytotoxicity. Even if the spleen cells were incubated with any concentration of alpha-endorphin or [Met]enkephalin indicated, the lytic activity remained unchanged. In contrast, only beta-endorphin resulted in a significant increase of the lytic activity between 0.5 nM and 50 nM. These results suggest that opioid peptides play a crucial role in immune surveillance mechanisms.

show abstract

“…Le traitement par la PGD2 de deux lignées ovariennes tumorales d'origine épithéliale (BG1) ou provenant de la granulosa (Cov434) induit une diminution de leur croissance et une augmentation de leur apoptose, via le récepteur DP1 et l'activation de l'expression de SOX9 ( Figure 3B) [45]. Une telle inhibition des cellules ovariennes KF (dérivées d'un adénocarcinome séreux humain) a été rapportée antérieurement in vitro [46] et in vivo dans des expériences de xénogreffes [47]. Par ailleurs, l'activation de l'expression de la L-PGDS par l'acide rétinoïque est responsable de l'inhibition de croissance des cellules ovariennes tumorales 3AO en culture [48].…”

Section: Revuesunclassified

La prostaglandine D2

Malki

Declosmenil²,

Farhat³

et al. 2008

Med Sci (Paris)

View full text Add to dashboard Cite

Inhibition of human ovarian cancer cell proliferation in vitro by neuroendocrine hormones

Cited by 24 publications

References 9 publications

Melatonin synergistically enhances cisplatin‐induced apoptosis via the dephosphorylation of ERK/p90 ribosomal S6 kinase/heat shock protein 27 in SK‐OV‐3 cells

Melatonin synergistically enhances cisplatin‐induced apoptosis via the dephosphorylation of ERK/p90 ribosomal S6 kinase/heat shock protein 27 in SK‐OV‐3 cells

Effects of opioid peptides on the cellular immunity in spleen cells from intact nude mice or nude mice bearing human ovarian carcinoma

La prostaglandine D2

Contact Info

Product

Resources

About