Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents

Abstract

Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyl eugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (<0.1%) amounts in one or both plant components. Screening of the essential oils for biological activity showed that AKEOstm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). We found that one component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by an inhibition of neutrophil migration and ROS production.

Modulation of Human Neutrophil Responses by the Essential Oils from Ferula akitschkensis and Their Constituents

“…Essential oil was obtained by hydrodistillation using a Clevenger type apparatus, as described previously 9 . For the hydrodistillation, we used conditions accepted by the European Pharmacopoeia (European Directorate for the Quality of Medicines, Council of Europe, Strasbourg, France, 2014) to avoid artifacts.…”

“…GC-MS analysis was performed with an Agilent 5975 GC-MSD system (Agilent Technologies, Santa Clara, CA, USA), as reported previously 9 . An Agilent Innowax FSC column (60 m × 0.25 mm, 0.25 µm film thickness) was used with He as the carrier gas (0.8 mL/min).…”

Antagonism of human formyl peptide receptor 1 with natural compounds and their synthetic derivatives

“…Lawsochylin A (Table 1) and (4 S )-4-hydroxy-α-tetralone isolated from Lawsonia inermis also inhibited f MLF-induced responses [73]. Recently, we found that 6-methyl-3,5-heptadien-2-one, which is a minor constituent in essential oils from Artemisia kotuchovii [196] and oleoresins from paprika and tomato [197], could non-specifically inhibit all functional responses that we tested in human neutrophils and FPR1-transfected HL-60 cells, including f MLF-stimulated Ca 2+ flux and chemotaxis, PMA-induced ROS production, and interleukin 8-induced Ca 2+ flux. We concluded that this compound may interfere with intracellular signaling pathways common to both FPR1 and CXCR1/2 [196].…”

“…Recently, we found that 6-methyl-3,5-heptadien-2-one, which is a minor constituent in essential oils from Artemisia kotuchovii [196] and oleoresins from paprika and tomato [197], could non-specifically inhibit all functional responses that we tested in human neutrophils and FPR1-transfected HL-60 cells, including f MLF-stimulated Ca 2+ flux and chemotaxis, PMA-induced ROS production, and interleukin 8-induced Ca 2+ flux. We concluded that this compound may interfere with intracellular signaling pathways common to both FPR1 and CXCR1/2 [196]. Viscolin and bractelactone, which are chalcones from Viscum coloratum and Fissistigma bracteolatum , respectively, also inhibited f MLF-induced O 2 −· production and HNE release by neutrophils; however, these compounds did not inhibit f MLF-induced Ca 2+ flux [198, 199], suggesting targets other than FPR1.…”

The impact of compounds isolated from Ocimum sp. on apoptotic activity of osteoclast

“…Schepetkin et al (2015) reported that Emethyl cinnamate was not effective in modulating some innate responses like neuthophil migration and ROS production. O. americanum essential oil contained about 55% of methyl cinnamate in its composition; however neither the essential oil extracted from O. americanum nor methyl cinnamate alone were able to induce apoptosis in OCs.…”