Inhibition of human cervical cancer cell invasion by IL-37 involving runt related transcription factor 2 suppression

Ouyang, Ping; Wu, Kun-Lung; Su, Liudan; An, Weifang; Bie, Yanhong; Zhang, He; Kang, Haixian; Jiang, Enping; Zhu, Wei; Yao, Yunhong; Hu, Xinrong; Chen, Zhangquan; Wang, Sen

doi:10.21037/atm.2019.09.38

Cited by 17 publications

(12 citation statements)

References 41 publications

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“…While in cervical cancer, knockdown of CCAT1 prevented the tumor progression by blocking the cell growth, migration, invasion and EMT by downregulating RUNX2 [24]. Additionally, upregulation of RUNX2 at least partly recovered the cell invasion reduced by IL-37 in cervical cancer cells [25]. Likewise, our in vivo experiments revealed that RUNX2 restoration counteracted the inhibitory effect of miR-130a-5p on tumor formation.…”

Section: Discussionmentioning

confidence: 52%

The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancer

Xie

Lei

et al. 2020

BMC Cancer

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Background Non-small cell lung cancer (NSCLC) remains a huge health burden for human health and life worldwide. Our study here was to illuminate the relevance of microRNA-130a-5p (miR-130a-5p) on growth and epithelial mesenchymal transition (EMT) in NSCLC cells along with metastasis in vivo, and to explore the underlying mechanism. Methods RT-qPCR was carried out for miR-130a-5p expression determination in NSCLC cells and tissue samples. Dual-luciferase reporter gene assay, RT-qPCR and western blot were carried out to study the potential targets of miR-130a-5p. Effects of miR-130a-5p, runt-related transcription factor 2 (RUNX2) and encoding serine/threonine kinase 32A (STK32A) on NSCLC proliferation, migration, invasion as well as EMT processes were assessed by cell counting kits-8, colony formation, Transwell and western blot assays. Results miR-130a-5p was diminished in NSCLC tissues and cells versus their counterparts. miR-130a-5p exerted its repressive role in NSCLC by curtailing cell viability, migration, invasion as well as EMT, while facilitating apoptosis. miR-130a-5p directly targeted RUNX2, a transcription factor, and conversely regulated its expression. RUNX2 was found to interact with STK32A to promote its expression. Following the validation of the supporting role of STK32A in NSCLC cells and NF-κB p65 phosphorylation, RUNX2 overexpression was monitored to reverse miR-130a-5p-inhibited NSCLC tumor volume and weight through enhancing STK32A expression in vivo. Conclusions miR-130a-5p diminished the growth and EMT of NSCLC cells by regulating the RUNX2/STK32A/NF-κB p65 axis, offering possible targets for the treatment for NSCLC.

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Section: Discussionmentioning

confidence: 52%

The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancer

Xie

Lei

et al. 2020

BMC Cancer

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“…Furthermore, runt related transcription factor 2 (RUNX2) is a member of the RUNX family that activates genes associated with tumorigenesis and metastasis, promoting tumor cell invasion in cancer. IL-37 overexpression significantly inhibits RUNX2 mRNA and protein expression (84), thus markedly inhibiting cell invasion.…”

Section: Suppression Of Cancer By Il-37 Il-37-induced Effects On Tumor Angiogenesis Migration and Progressionmentioning

confidence: 97%

Current Understanding of IL-37 in Human Health and Disease

Tao

2021

Front. Immunol.

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IL-37 is a recently discovered cytokine in the IL-1 family exerting broad protective effects on inflammatory diseases, autoimmune diseases, and cancer. Immune and non-immune cells produce the IL-37 precursor upon pro-inflammatory stimuli. Intracellularly, caspase-1 cleaves and activates IL-37, and its mature form binds to Smad3; this complex translocates into the nucleus where it suppresses cytokine production, consequently reducing inflammation. Extracellularly, IL-37 forms a complex with IL-18Rα and IL-1R8 (formerly TIR8 or SIGIRR) that transduces anti-inflammatory signals by the suppression of NF-κB and MAPK and the activation of Mer-PTEN-DOK pathways. During inflammation, IL-37 suppresses the expression of several pro-inflammatory cytokine in favor to the expression of the anti-inflammatory ones by the regulation of macrophage polarization, lipid metabolism, inflammasome function, TSLP synthesis and miRNAs function. Moreover, IL-37 not only regulates the innate and acquired immunity, but also improves aging-associated immunosenescence. Furthermore, IL-37 exerts an inhibitory effect on tumor angiogenesis and metastasis, and progression. Finally, IL-37 may have a potential ability to reduce excessive inflammation since it is aberrantly expressed in patients with inflammatory diseases, autoimmune diseases, and cancer, thus, it may be used as a marker for different types of diseases. Therefore, this review provides an updated view of the role of IL-37 in human health and disease, and discusses the potential of IL-37 as a therapeutic target and biomarker in inflammatory diseases, autoimmune diseases, and cancer.

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“…While in cervical cancer, knockdown of CCAT1 prevented the tumor progression by blocking the cell growth, migration, invasion and EMT by downregulating RUNX2 [24]. Additionally, upregulation of RUNX2 at least partly recovered the cell invasion reduced by IL-37 in cervical cancer cells [25]. Likewise, out in vivo experiments revealed that RUNX2 restoration counteract the inhibitory effect of miR-130a-5p on tumor formation.…”

Section: Discussionmentioning

confidence: 91%

The MicroRNA-130a-5p/RUNX2/STK32A Network Modulates Tumor Invasive and Metastatic Potential in Non-Small Cell Lung Cancer

Xie

Lei

et al. 2020

Preprint

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Background Non-small cell lung cancer (NSCLC) remains a huge health burden for human health and life worldwide. Our study here was to illuminate the relevance of microRNA-130a-5p (miR-130a-5p) on growth and epithelial mesenchymal transition (EMT) in NSCLC cells as along with metastasis in vivo, and to explore the underlying mechanism.Methods RT-qPCR was carried out for miR-130a-5p expression determination in NSCLC cells and tissue samples. Dual luciferase reporter gene assay, RT-qPCR and western blot were carried out to study the potential targets of miR-130a-3p. Effects of miR-130a-5p, runt-related transcription factor 2 (RUNX2) and encoding serine/threonine kinase 32A (STK32A) on NSCLC proliferation, migration, invasion as well as EMT processes were assessed by cell counting kits-8, colony formation, Transwell and western blot assays.Results miR-130a-5p was diminished in NSCLC tissues and cells versus their counterparts. miR-130a-5p exerted its repressive role in NSCLC by curtailing cell viability, migration, invasion as well as EMT, while facilitating apoptosis. miR-130a-5p directly targeted RUNX2, a transcription factor, and conversely regulated its expression. RUNX2 was found to interacted with STK32A to promote its expression. Following the validation of the tumor-supporting role of STK32A in NSCLC cells, RUNX2 overexpression was monitored to reverse miR-130a-5p-inhibited NSCLC tumor volume and weight through enhancing STK32A expression in vivo.Conclusions miR-130a-5p diminished the growth and EMT of NSCLC cells by regulating the RUNX2/STK32A axis, offering possible targets for the treatment for NSCLC.

show abstract

Inhibition of human cervical cancer cell invasion by IL-37 involving runt related transcription factor 2 suppression

Cited by 17 publications

References 41 publications

The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancer

The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancer

Current Understanding of IL-37 in Human Health and Disease

The MicroRNA-130a-5p/RUNX2/STK32A Network Modulates Tumor Invasive and Metastatic Potential in Non-Small Cell Lung Cancer

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