Inhibition of human brain malignant glioblastoma cells using carmustine-loaded catanionic solid lipid nanoparticles with surface anti-epithelial growth factor receptor

Kuo, Yung‐Chih; Liang, Cheng-Te

doi:10.1016/j.biomaterials.2011.01.048

Cited by 105 publications

(55 citation statements)

References 51 publications

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“…As a promising drug-delivery system, SLNs displayed many important advantages, such as controlling drug release and drug targeting, increasing physical stability, high drug loading, and low toxicity. 33,34 Here, we hypothesized that SLN formulation of curcumin was capable of overcoming the above-mentioned drawbacks of curcumin. In the current study, curcumin-SLNs were developed to serve as an alternative formulation of curcumin for a more effective therapy of asthma.…”

Section: Discussionmentioning

confidence: 99%

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Wang

Zhu²,

Xie³

et al. 2012

IJN

172

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Curcumin has shown considerable pharmacological activity, including anti-inflammatory, but its poor bioavailability and rapid metabolization have limited its application. The purpose of the present study was to formulate curcumin-solid lipid n anoparticles (curcumin-SLNs) to improve its therapeutic efficacy in an ovalbumin (OVA)-induced allergic rat model of asthma. A solvent injection method was used to prepare the curcumin-SLNs. Physiochemical properties of curcumin-SLNs were characterized, and release experiments were performed in vitro. The pharmacokinetics in tissue distribution was studied in mice, and the therapeutic effect of the formulation was evaluated in the model. The prepared formulation showed an average size of 190 nm with a zeta potential value of −20.7 mV and 75% drug entrapment efficiency. X-ray diffraction analysis revealed the amorphous nature of the encapsulated curcumin. The release profile of curcumin-SLNs was an initial burst followed by sustained release. The curcumin concentrations in plasma suspension were significantly higher than those obtained with curcumin alone. Following administration of the curcumin-SLNs, all the tissue concentrations of curcumin increased, especially in lung and liver. In the animal model of asthma, curcumin-SLNs effectively suppressed airway hyperresponsiveness and inflammatory cell infiltration and also significantly inhibited the expression of T-helper-2-type cytokines, such as interleukin-4 and interleukin-13, in bronchoalveolar lavage fluid compared to the asthma group and curcumin-treated group. These observations implied that curcumin-SLNs could be a promising candidate for asthma therapy.

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Section: Discussionmentioning

confidence: 99%

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Wang

Zhu²,

Xie³

et al. 2012

IJN

172

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“…22,23 Free drugs or 0.025% (w/v) LIP carriers in culture medium were added to the upper donor chamber and placed in a humidified CO 2 incubator (NuAire, Plymouth, MN, USA) at 37°C for 4 h. A Millicell system (Millipore) was used to evaluate the electrical resistances of cell-free and monolayer-containing membrane. The transendothelial electrical resistance (TEER) of the monolayer was defined as (electrical resistance of monolayer-containing membrane − electrical resistance of cell-free membrane) × surface area of the membrane.…”

Section: Stability Of Lip Carriersmentioning

confidence: 99%

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment

Kuo¹,

Lin²,

Li³

et al. 2017

IJN

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Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer’s disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells. In addition, NGF-CL/LIP could enhance the quantities of p-neurotrophic tyrosine kinase receptor type 1 and p-extracellular signal-regulated kinase 5 for neuronal rescue. Moreover, WGA-grafted CRM-CL/LIP and WGA-grafted NGF-CL/LIP significantly improved the permeation of CRM and NGF across the blood–brain barrier, reduced Aβ plaque deposition and the malondialdehyde level, and increased the percentage of normal neurons and cholinergic activity in the hippocampus of AD rats. Based on the marker expressions and in vivo evidence, current LIP carriers can be promising drug delivery systems to protect nervous tissue against Aβ-induced apoptosis in the brain during the clinical management of AD.

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“…This pyridyldithio (PDT)-activated PEI was named PEI-PDT 10 . For the other two groups (PEI-PDT 20 and PEI-PDT 40 ), the same protocol was used, except the volumes of SPDP used were 200 µL and 400 µL, respectively. The PEI-PDT polymers were purified using a PD-10 column, according to the manufacturer's instructions.…”

Section: Synthesis Of Vtw-modified Pei Copolymersmentioning

confidence: 99%

“…Then, the VTW solution with different molar ratios to PEI-PDT (10:1, 20:1, 40:1) was dropped into three types of PEI-PDT solutions and incubated for 2 hours at RT. The PDT residue of PEI-PDT was displaced by the sulfhydryls of the cysteine residue of VTW, forming the VTW-modified PEI copolymers (PEI-VTW, here abbreviated as PV 10 , PV 20 and PV 40 ). Subsequently, the copolymers were purified separately by using a 30 kDa cutoff filter (Vivaspin 2; Sartorius, Göttingen, Germany) and centrifugation at 12,000 Xg for 20 minutes.…”

Section: Synthesis Of Vtw-modified Pei Copolymersmentioning

confidence: 99%

“…14,16,17 Certain molecules overexpressed on the surface of some tumor cells, including fibroblast growth factor receptors and epidermal growth factor receptors, have been used to improve the penetration of drugs or genetic products into tumor cells. [18][19][20] However, these molecules are not specific enough because they are also expressed on some normal cells, although not to the same extent as on tumor cells. Phage peptide display is one technique that can be used to isolate peptides with targeting effects on specific cells.…”

Section: Introductionmentioning

confidence: 99%

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A peptide-mediated targeting gene delivery system for malignant glioma cells

Wang¹,

Ning

Wang³

et al. 2013

IJN

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Glioblastoma multiforme (GBM) is the most common and malignant glioma. Although there has been considerable progress in treatment strategies, the prognosis of many patients with GBM remains poor. In this work, polyethylenimine (PEI) and the VTWTPQAWFQWV (VTW) peptide were modified and synthesized into GBM-targeting nanoparticles. The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. Cell internalization was investigated to verify the nanoparticle delivery into the cytoplasm. Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. The PEI-VTW 20 nanoparticles increased the transfection efficiency significantly. This report describes the use of VTW peptide-based PEI nanoparticles for intracellular gene delivery in a GBM cell-specific manner.

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Inhibition of human brain malignant glioblastoma cells using carmustine-loaded catanionic solid lipid nanoparticles with surface anti-epithelial growth factor receptor

Cited by 105 publications

References 51 publications

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment

A peptide-mediated targeting gene delivery system for malignant glioma cells

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Inhibition of human brain malignant glioblastoma cells using carmustine-loaded catanionic solid lipid nanoparticles with surface anti-epithelial growth factor receptor

Cited by 105 publications

References 51 publications

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer&rsquo;s disease treatment

A peptide-mediated targeting gene delivery system for malignant glioma cells

Contact Info

Product

Resources

About

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment