Inhibition of Human 20S Proteasome by Ginsenosides from Panax ginseng

Shim, Sang Hee; Baek, Kwang‐Hyun; Kim, Yeong Shik

doi:10.5012/bkcs.2009.30.6.1385

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…As shown in Figure 7 B, Rh2 is a dose-dependent activator of the Neh2-luc reporter, providing a two-fold stabilization of the Neh2-luc fusion protein after 3 h incubation. The effect is specific for the Nrf2 reporter system, since no activation is observed for HIF1 ODD-luc reporter used as a control for assay specificity, ruling out the common effects such as inhibition of ubiquitin activating enzyme [ 20 ] or proteasome [ 21 ] in the range of Rh2 concentrations used. Since no activation effect was observed for 20S-protopanaxadiol in the reporter system at 3 h incubation (results not shown), one may expect that the glucopyranoside ring in Rh2 works toward an improved interaction with Keap1.…”

Section: Resultsmentioning

confidence: 99%

Probable Mechanisms of Doxorubicin Antitumor Activity Enhancement by Ginsenoside Rh2

et al. 2022

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Ginsenoside Rh2 increases the efficacy of doxorubicin (DOX) treatment in murine models of solid and ascites Ehrlich’s adenocarcinoma. In a solid tumor model (treatment commencing 7 days after inoculation), DOX + Rh2 co-treatment was significantly more efficacious than DOX alone. If treatment was started 24 h after inoculation, the inhibition of tumor growth of a solid tumor for the DOX + Rh2 co-treatment group was complete. Furthermore, survival in the ascites model was dramatically higher for the DOX + Rh2 co-treatment group than for DOX alone. Mechanisms underlying the combined DOX and Rh2 effects were studied in primary Ehrlich’s adenocarcinoma-derived cells and healthy mice’s splenocytes. Despite the previously established Rh2 pro-oxidant activity, DOX + Rh2 co-treatment revealed no increase in ROS compared to DOX treatment alone. However, DOX + Rh2 treatment was more effective in suppressing Ehrlich adenocarcinoma cell adhesion than either treatment alone. We hypothesize that the benefits of DOX + Rh2 combination treatment are due to the suppression of tumor cell attachment/invasion that might be effective in preventing metastatic spread of tumor cells. Ginsenoside Rh2 was found to be a modest activator in a Neh2-luc reporter assay, suggesting that Rh2 can activate the Nrf2-driven antioxidant program. Rh2-induced direct activation of Nrf2 might provide additional benefits by minimizing DOX toxicity towards non-cancerous cells.

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Section: Resultsmentioning

confidence: 99%

Probable Mechanisms of Doxorubicin Antitumor Activity Enhancement by Ginsenoside Rh2

et al. 2022

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20S proteasome inhibitory activity of flavonoids isolated from Spatholobus suberectus

Shim

2010

Phytotherapy Research

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The promising biological role of the ubiquitin proteasome pathway (UPP) in cancer therapy has recently emerged. Inhibition of proteasome has been proposed as a new therapeutic target for treatment of cancer. Bioassay-guided fractionation of a MeOH extract of the stems of Spatholobus suberectus resulted in seven compounds, liquiritigenin (1), isoliquiritigenin (2), genistein (3), daidzein (4), medicarpin (5), 7-hydroxyflavanone (6) and formononetin (7), which were evaluated for the first time for their inhibitory effect on 20S proteasome. Among the isolated compounds, 2, 3 and 6 exhibited inhibitory activities on human 20S proteasome with IC(50) values of 4.88 ± 1.5, 9.26 ± 1.2 and 5.21 ± 1.5 µm, respectively.

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Modification of ginsenoside saponin composition via the CRISPR/Cas9-mediated knockout of protopanaxadiol 6-hydroxylase gene in Panax ginseng

Choi

Koo

Jeon

et al. 2022

Journal of Ginseng Research

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Inhibition of Human 20S Proteasome by Ginsenosides from Panax ginseng

Cited by 4 publications

References 24 publications

Probable Mechanisms of Doxorubicin Antitumor Activity Enhancement by Ginsenoside Rh2

Probable Mechanisms of Doxorubicin Antitumor Activity Enhancement by Ginsenoside Rh2

20S proteasome inhibitory activity of flavonoids isolated from Spatholobus suberectus

Modification of ginsenoside saponin composition via the CRISPR/Cas9-mediated knockout of protopanaxadiol 6-hydroxylase gene in Panax ginseng

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