Inhibition of Hsp90 and 70 sensitizes melanoma cells to hyperthermia using ferromagnetic particles with a low Curie temperature

Miyagawa, Tomoyuki; Saito, Hajime; Minamiya, Yoshihiro; Mitobe, Kazutaka; Takashima, Shinogu; Takahashi, Naoko; Ito, Aki; Imai, Kazuhiro; Motoyama, Satoru; Ogawa, Jun–ichi

doi:10.1007/s10147-013-0606-x

Cited by 42 publications

(35 citation statements)

References 44 publications

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“…Studies have confirmed that heat-induced HSP90 expression protects cells by preventing protein degradation, and thus leads to thermoresistance. 23,24 Although cancer stem cells show poor response to radiotherapy and chemotherapy, thermotherapy can effectively eliminate cancer stem cell and inhibit their stem cell-like properties. 13 In this study, inhibiting the thermoresistance of CD90 + LCSCs markedly enhanced the thermal-induced apoptosis.…”

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confidence: 99%

Inhibition of heat-shock protein 90 sensitizes liver cancer stem-like cells to magnetic hyperthermia and enhances anti-tumor effect on hepatocellular carcinoma-burdened nude mice

Yang¹,

Tang²,

Miao³

et al. 2015

IJN

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Purpose To explore the thermoresistance and expression of heat-shock protein 90 (HSP90) in magnetic hyperthermia-treated human liver cancer stem-like cells (LCSCs) and the effects of a heat-shock protein HSP90 inhibitor 17-allylamino-17-demethoxgeldanamycin (17-AAG) on hepatocellular carcinoma-burdened nude mice. Methods CD90 + LCSCs were isolated by magnetic-activated cell sorting from BEL-7404. Spheroid formation, proliferation, differentiation, drug resistance, and tumor formation assays were performed to identify stem cell characteristics. CD90-targeted thermosensitive magnetoliposomes (TMs)-encapsulated 17-AAG (CD90@17-AAG/TMs) was prepared by reverse-phase evaporation and its characteristics were studied. Heat tolerance in CD90 + LCSCs and the effect of CD90@17-AAG/TMs-mediated heat sensitivity were examined in vitro and in vivo. Results CD90 + LCSCs showed significant stem cell-like properties. The 17-AAG/TMs were successfully prepared and were spherical in shape with an average size of 128.9±7.7 nm. When exposed to magnetic hyperthermia, HSP90 was up-regulated in CD90 + LCSCs. CD90@17-AAG/TMs inhibited the activity of HSP90 and increased the sensitivity of CD90 + LCSCs to magnetic hyperthermia. Conclusion The inhibition of HSP90 could sensitize CD90 + LCSCs to magnetic hyperthermia and enhance its anti-tumor effects in vitro and in vivo.

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confidence: 99%

Inhibition of heat-shock protein 90 sensitizes liver cancer stem-like cells to magnetic hyperthermia and enhances anti-tumor effect on hepatocellular carcinoma-burdened nude mice

Yang¹,

Tang²,

Miao³

et al. 2015

IJN

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“…We previously developed a thermo‐sensitive ferromagnetic implant with low Curie temperature (FILCT) with a particle diameter of 50–150 μm and a Curie point of 43 °C, which is a suitable temperature for treatment . Next, we confirmed that this approach enhanced the antitumor effect and improved the survival rate when combined with a heat shock protein 90 inhibitor in a rat melanoma model . However, because of the low heating efficiency of the FILCT, it was difficult to heat up to 43 °C when treating a human body.…”

Section: Introductionmentioning

confidence: 75%

Position adjustment method and distance estimation method of magnetic field supply and detection unit for magnetic hyperthermia

Tonthat

Aki

Matsuda

et al. 2017

IEEJ Transactions Elec Engng

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In magnetic hyperthermia, the temperature and localization of a ferromagnetic implant in a tumor region is vital in determining the effectiveness of therapeutic heating. We have developed a noninvasive wireless temperature measurement method by utilizing the magnetic permeability property of a ferromagnetic implant with low Curie temperature (FILCT) that varies with the temperature. In clinical settings, when the FILCT is injected into a tumor region, the position of the FILCT is expected to deviate from the central axis of the heating coil (drive coil). When it deviates, the magnetic flux density applied to the FILCT decreases, thus causing difficulty in detecting the temperature of FILCT via a pickup coil and heating of the FILCT at a constant treatment temperature. Therefore, to adjust the magnetic field supply and detection (MFSD) unit to be directly above the FILCT, we propose a position adjustment method by referring to three voltages induced in three pickup coils symmetrically installed inside the drive coil. By using the average value of the three voltages, we also propose a method to estimate the distance between the MFSD unit and the FILCT, which is required for wireless thermometry and determination of the optimal energy applied. The proposed methods are experimentally examined by a verification system.

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“…58 An important mediator of the cell stress response is heat shock protein 90 (Hsp90), which has been reported to be upregulated in melanoma, 59 and its inhibition, along with the inhibition of Hsp70, has been reported to enhance the sensitivity of melanoma cells to the anti-tumor effects of hyperthermia. 60 Interestingly, there is evidence showing that curcumin synergistically enhances the anti-tumor effect of bortezomib in melanoma through inhibition of Hsp90 expression. 61 Hemeoxygenase/biliverdin reductase (HO/BVR) is another main component of the cell stress response.…”

Section: Curcumin As a Therapeutic Agent In Melanomamentioning

confidence: 99%

Curcumin: A new candidate for melanoma therapy?

Mirzaei

Naseri

Rezaee

et al. 2016

Intl Journal of Cancer

225

195

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Melanoma remains among the most lethal cancers and, in spite of great attempts that have been made to increase the life span of patients with metastatic disease, durable and complete remissions are rare. Plants and plant extracts have long been used to treat a variety of human conditions; however, in many cases, effective doses of herbal remedies are associated with serious adverse effects. Curcumin is a natural polyphenol that shows a variety of pharmacological activities including anti-cancer effects, and only minimal adverse effects have been reported for this phytochemical. The anti-cancer effects of curcumin are the result of its anti-angiogenic, pro-apoptotic and immunomodulatory properties. At the molecular and cellular level, curcumin can blunt epithelial-to-mesenchymal transition and affect many targets that are involved in melanoma initiation and progression (e.g., BCl2, MAPKS, p21 and some microRNAs). However, curcumin has a low oral bioavailability that may limit its maximal benefits. The emergence of tailored formulations of curcumin and new delivery systems such as nanoparticles, liposomes, micelles and phospholipid complexes has led to the enhancement of curcumin bioavailability. Although in vitro and in vivo studies have demonstrated that curcumin and its analogues can be used as novel therapeutic agents in melanoma, curcumin has not yet been tested against melanoma in clinical practice. In this review, we summarized reported anti-melanoma effects of curcumin as well as studies on new curcumin formulations and delivery systems that show increased bioavailability. Such tailored delivery systems could pave the way for enhancement of the anti-melanoma effects of curcumin.

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Inhibition of Hsp90 and 70 sensitizes melanoma cells to hyperthermia using ferromagnetic particles with a low Curie temperature

Abstract: Combining Hsp90/70 inhibition with hyperthermia appears to increase their antitumor effects. Thus, the combination of FMP-mediated, self-regulating hyperthermia with Hsp90/70 inhibition has important implications for cancer treatment.

Cited by 42 publications

References 44 publications

Inhibition of heat-shock protein 90 sensitizes liver cancer stem-like cells to magnetic hyperthermia and enhances anti-tumor effect on hepatocellular carcinoma-burdened nude mice

Inhibition of heat-shock protein 90 sensitizes liver cancer stem-like cells to magnetic hyperthermia and enhances anti-tumor effect on hepatocellular carcinoma-burdened nude mice

Position adjustment method and distance estimation method of magnetic field supply and detection unit for magnetic hyperthermia

Curcumin: A new candidate for melanoma therapy?

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