Inhibition of Homodimerization of Toll-like Receptor 4 by 6-Shogaol

Ahn, Soo Whan; Lee, Jun-Kyung; Youn, Hyung‐Sun

doi:10.1007/s10059-009-0026-y

Cited by 80 publications

(61 citation statements)

References 26 publications

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“…Additionally, Ahn et al showed that ginger inhibits homodimerization of TLR4 which suppress its functions [25]. Another study revealed that ginger suppresses expression of inflammatory genes in a NF-κB dependent manner [26]. NF-κB is a main transcription factor down-stream of TLR2 and 4 and participates in the expression of the TLRs [27][28][29].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Alcoholic Extract of Ginger on Apoptosis and Inflammation of Germ Cells in Streptozotocin-Induced Diabetic Rats

Mt¹,

Sk²,

T³

et al. 2018

Asian J Biomed Pharmaceut Sci

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Background: Herbal drugs have been used to treat several diseases from liver and neural disorders to diabetes. Our study was aimed to find out the effects of alcoholic ginger extract on testis/body weight and apoptosis of sperm germ cells of chronic diabetic rat. Methods: In this experimental study, sixty male Wistar rats were randomly divided into 5 groups: nontreatment normal (group 1), non-treatment diabetic (group 2), ginger treatment diabetic (group 3), insulin treatment diabetic (group 4) and ginger plus insulin treatment diabetic (group 5). Ginger alcohol extract was injected intraperitoneally at the dose of 200 mg/kg for 6 weeks. TUNEL method was employed to evaluate the germ cells apoptosis. Expression of Bax, Bcl-2, CyclinD1, TRPV1, TLR2 and TLR4 was evaluated using Real-Time PCR technique. Results: Results revealed a significant decrease in the apoptosis index in the group 5 with respect to the group 2 (P<0.05). The expression of BAX, BCL2, TRPV1, TLR2 and TLR4 significantly decreased, while CYC D1 was increased in the group 5 in comparison to group 2. Conclusions: These results suggest ginger has a potentially beneficial role in reducing apoptosis and inflammation in testicular germ cells in chronic diabetic adult rats.

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Section: Discussionmentioning

confidence: 99%

The Effect of Alcoholic Extract of Ginger on Apoptosis and Inflammation of Germ Cells in Streptozotocin-Induced Diabetic Rats

Mt¹,

Sk²,

T³

et al. 2018

Asian J Biomed Pharmaceut Sci

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“…Protein extracts from Ba/F3 cells expressing TLR4 (Flag or GFP-tagged), CD14, MD2 (Flag-tagged), and NF-κB luciferase reporter gene were prepared for immunoprecipitation as described previously 8,11 . Briefly, the samples were immunoprecipitated by an overnight application of mouse-green fluorescent protein (GFP) antibody (Molecular Probes, Eugene, OR, USA).…”

Section: Immunoprecipitationmentioning

confidence: 99%

Inhibition of homodimerization of toll-like receptor 4 by (E)-isopropyl 4-oxo-4-(2-oxopyrrolidin-1-yl)-2-butenoate

Park

Kim

Shin

et al. 2011

Toxicol. Environ. Health Sci.

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Toll-like receptors (TLRs) recognize molecular structures derived from microbes and initiate innate immunity. The stimulation of TLR4 by lipopolysaccharide triggers the activation of the myeloid differential factor 88 (MyD88)-and toll-interleukin-1 receptor domain -containing adapter inducing interferon-β (TRIF)-dependent signaling pathways. Previously, we synthesized a fumaryl pyrrolidinone, (E)-isopropyl 4-oxo-4-(2-oxopyrrolidin-1-yl)-2-butenoate (IPOP) and demonstrated that it inhibits activation of nuclear factor kappa B (NF-κB) by inhibiting the MyD88-dependent pathway of TLRs. TLR4 and the downstream signaling components are good therapeutic targets for many chronic inflammatory diseases. Here, it is investigated whether IPOP modulates TLR4 downstream signaling pathways and what anti-inflammatory target in TLR4 signaling is regulated by IPOP. IPOP inhibited LPSinduced NF-κB and interferon regulatory factor 3 (IRF3) activation by targeting TLR4 dimerization. These results suggest that IPOP can modulate TLR4 signaling pathway at the receptor level to decrease inflammatory gene expression.

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“…The assays were performed as described previously (17,18). Briefly, RAW264.7 cells were co-transfected with a luciferase plasmid and HSP70-β-gal plasmid as an internal control using SuperFect transfection reagent (Qiagen) according to the manufacturer's instructions.…”

Section: Transfection and Luciferase Assaymentioning

confidence: 99%

Inhibition of Homodimerization of Toll-like Receptor 4 by 6-Shogaol

Cited by 80 publications

References 26 publications

The Effect of Alcoholic Extract of Ginger on Apoptosis and Inflammation of Germ Cells in Streptozotocin-Induced Diabetic Rats

The Effect of Alcoholic Extract of Ginger on Apoptosis and Inflammation of Germ Cells in Streptozotocin-Induced Diabetic Rats

Inhibition of homodimerization of toll-like receptor 4 by (E)-isopropyl 4-oxo-4-(2-oxopyrrolidin-1-yl)-2-butenoate

Suppression of the TRIF-dependent signaling pathway of toll-like receptors by (E)-isopropyl 4-oxo-4-(2-oxopyrrolidin-1-yl)-2-butenoate

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