Inhibition of hKv2.1, a major human neuronal voltage-gated K+ channel, by meclofenamic acid

Lee, Yan T.; Wang, Qiang

doi:10.1016/s0014-2999(99)00485-9

Cited by 46 publications

(39 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S5F, S5G), as expected for the involvement of gap junctions coupling between similar cell types. These effects of MFA on passive membrane properties were specifically observed in precursors in contact but not in isolated precursors (Table 1) or neuroblasts (data not shown), suggesting that they are most likely due to a specific block of gap junctions rather than to a nonspecific effect of this drug [31].…”

Section: Functional Properties Of Precursors and Neuroblastsmentioning

confidence: 91%

Analysis of Stem Cell Lineage Progression in the Neonatal Subventricular Zone Identifies EGFR+/NG2− Cells as Transit-Amplifying Precursors

Cesetti¹,

Obernier²,

Bengtson³

et al. 2009

Stem Cells

View full text Add to dashboard Cite

In the adult subventricular zone (SVZ), astroglial stem cells generate transit-amplifying precursors (TAPs). Both stem cells and TAPs form clones in response to epidermal growth factor (EGF). However, in vivo, in the absence of sustained EGF receptor (EGFR) activation, TAPs divide a few times before differentiating into neuroblasts. The lack of suitable markers has hampered the analysis of stem cell lineage progression and associated functional changes in the neonatal germinal epithelium. Here we purified neuroblasts and clone-forming precursors from the neonatal SVZ using expression levels of EGFR and polysialylated neural cell adhesion molecule (PSANCAM). As in the adult SVZ, most neonatal clone-forming precursors did not express the neuroglia proteoglycan 2 (NG2) but displayed characteristics of TAPs, and only a subset exhibited antigenic characteristics of astroglial stem cells. Both precursors and neuroblasts were PSANCAM 1 ; however, neuroblasts also expressed doublecortin and functional voltage-dependent Ca 21 channels. Neuroblasts and precursors had distinct outwardly rectifying K 1 current densities and passive membrane properties, particularly in precursors contacting each other, because of the contribution of gap junction coupling. Confirming the hypothesis that most are TAPs, cell tracing in brain slices revealed that within 2 days the majority of EGFR 1 cells had exited the cell cycle and differentiated into a progenitor displaying intermediate antigenic and functional properties between TAPs and neuroblasts. Thus, distinct functional and antigenic properties mark stem cell lineage progression in the neonatal SVZ.

show abstract

Section: Functional Properties Of Precursors and Neuroblastsmentioning

confidence: 91%

Analysis of Stem Cell Lineage Progression in the Neonatal Subventricular Zone Identifies EGFR+/NG2− Cells as Transit-Amplifying Precursors

Cesetti¹,

Obernier²,

Bengtson³

et al. 2009

Stem Cells

View full text Add to dashboard Cite

show abstract

“…In the literature, many other effects have been attributed to FFA including block (Tesfai et al 2001;Lee et al 2003b;Guinamard et al 2004) or even stimulation of some TRP channels (Inoue et al 2001;Pocock et al 2004). Further targets of fenamates like FFA include chloride channels (Kim et al 2003), voltage gated Na + and K + channels (Lee and Wang 1999) and GABA A receptors (Sinkkonen et al 2003); in part the effective concentrations were similar as on TRPM2. Thus, FFA should be considered at best a starting point for the development of channel blockers that might be useful not only in the lab but in future also in clinical settings.…”

Section: Block Of Camentioning

confidence: 97%

Regulation of TRPM2 channels in neutrophil granulocytes by ADP-ribose: a promising pharmacological target

Heiner

Radukina

Eisfeld

et al. 2005

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

TRPM2 channels play an important role in the activation process of neutrophil granulocytes. One mechanism of TRPM2 channel gating is the binding of intracellular ADP ribose (ADPR) to the Nudix box domain in the C-terminal tail of TRPM2. Intracellular Ca 2+ , although not an activator of TRPM2 by its own, significantly enhances TRPM2 gating by ADPR. Stimulation of neutrophil granulocytes with the chemoattractant peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) induces release of Ca 2+ ions from intracellular stores which in cooperation with endogenous ADPR levels enable Ca 2+

show abstract

“…These preliminary results indeed suggest a critical role for gap junctions in mediating oscillations. However, it is not clear if the effects of MFA on ganglion cell activity arises from blockade of gap junctions or from secondary anticonvulsant actions (Lee andWang, 1999, Peretz et al, 2005). The evaluation of activity in transgenic rd1 mouse lines, in which specific connexins are selectively knocked out, will reveal the importance of gap junctions in mediating oscillatory activity.…”

Section: Mechanisms Driving Autonomous Activitymentioning

confidence: 99%

An Intrinsic Neural Oscillator in the Degenerating Mouse Retina

Borowska¹,

Trenholm²,

Awatramani³

2011

J. Neurosci.

107

171

View full text Add to dashboard Cite

The loss of photoreceptors during retinal degeneration (RD) is known to lead to an increase in basal activity in remnant neural networks. To identify the source of activity, we combined two-photon imaging with patch-clamp techniques to examine the physiological properties of morphologically identified retinal neurons in a mouse model of RD (rd1). Analysis of activity in rd1 ganglion cells revealed sustained oscillatory (ϳ10 Hz) synaptic activity in ϳ30% of all classes of cells. Oscillatory activity persisted after putative inputs from residual photoreceptor, rod bipolar cell, and inhibitory amacrine cell synapses were pharmacologically blocked, suggesting that presynaptic cone bipolar cells were intrinsically active. Examination of presynaptic rd1 ON and OFF bipolar cells indicated that they rested at relatively negative potentials (less than Ϫ50 mV). However, in approximately half the cone bipolar cells, low-amplitude membrane oscillation (ϳ5 mV, ϳ10 Hz) were apparent. Such oscillations were also observed in AII amacrine cells. Oscillations in ON cone bipolar and AII amacrine cells exhibited a weak apparent voltage dependence and were resistant to blockade of synaptic receptors, suggesting that, as in wild-type retina, they form an electrically coupled network. In addition, oscillations were insensitive to blockers of voltage-gated Ca 2ϩ channels (0.5 mM Cd 2ϩ and 0.5 mM Ni 2ϩ ), ruling out known mechanisms that underlie oscillatory behavior in bipolar cells. Together, these results indicate that an electrically coupled network of ON cone bipolar/AII amacrine cells constitutes an intrinsic oscillator in the rd1 retina that is likely to drive synaptic activity in downstream circuits.

show abstract

Inhibition of hKv2.1, a major human neuronal voltage-gated K+ channel, by meclofenamic acid

Cited by 46 publications

References 21 publications

Analysis of Stem Cell Lineage Progression in the Neonatal Subventricular Zone Identifies EGFR+/NG2− Cells as Transit-Amplifying Precursors

Analysis of Stem Cell Lineage Progression in the Neonatal Subventricular Zone Identifies EGFR+/NG2− Cells as Transit-Amplifying Precursors

Regulation of TRPM2 channels in neutrophil granulocytes by ADP-ribose: a promising pharmacological target

An Intrinsic Neural Oscillator in the Degenerating Mouse Retina

Contact Info

Product

Resources

About