Inhibition of HIV-1 Replication by Peptidic Protease Inhibitors

Petry, Harald; Ast, Oliver; Rosny, Eve de; Lieder, K.; Jentsch, Klaus Dieter; Hunsmann, Gerhard; Goldmann, Claudia; Lüke, Wolfgang; Büttner, J.; Reboud, M.; Schramm, W.; Schramm, Hans J.

doi:10.1007/978-3-642-59683-4_11

Cited by 8 publications

(10 citation statements)

References 12 publications

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“…In parallel, understanding fundamental determinants of budding and membrane dynamics would facilitate the design of viral nanoparticles. There is keen interest in reengineering enveloped viral nanoparticles to be used as targeted transport vehicles capable of crossing cell membranes through viral fusion [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Why Enveloped Viruses Need Cores—The Contribution of a Nucleocapsid Core to Viral Budding

Lázaro

Mukhopadhyay

Hagan

2018

Biophysical Journal

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During the lifecycle of many enveloped viruses, a nucleocapsid core buds through the cell membrane to acquire an outer envelope of lipid membrane and viral glycoproteins. However, the presence of a nucleocapsid core is not required for assembly of infectious particles. To determine the role of the nucleocapsid core, we develop a coarse-grained computational model with which we investigate budding dynamics as a function of glycoprotein and nucleocapsid interactions, as well as budding in the absence of a nucleocapsid. We find that there is a transition between glycoprotein-directed budding and nucleocapsid-directed budding that occurs above a threshold strength of nucleocapsid interactions. The simulations predict that glycoprotein-directed budding leads to significantly increased size polydispersity and particle polymorphism. This polydispersity can be explained by a theoretical model accounting for the competition between bending energy of the membrane and the glycoprotein shell. The simulations also show that the geometry of a budding particle leads to a barrier to subunit diffusion, which can result in a stalled, partially budded state. We present a phase diagram for this and other morphologies of budded particles. Comparison of these structures against experiments could establish bounds on whether budding is directed by glycoprotein or nucleocapsid interactions. Although our model is motivated by alphaviruses, we discuss implications of our results for other enveloped viruses.

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Section: Introductionmentioning

confidence: 99%

Why Enveloped Viruses Need Cores—The Contribution of a Nucleocapsid Core to Viral Budding

Lázaro

Mukhopadhyay

Hagan

2018

Biophysical Journal

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“…On the surface of recombinant viral capsids, molecules can be presented which show specific interactions and allows a wide variety of applications. Currently (modified) virus particles are in the center of investigations in peptide production, vaccinology, drug delivery and gene therapy . Moreover, in many fields of application the miniaturization of elements is of increasing importance.…”

Section: Discussionmentioning

confidence: 99%

A simple method to estimate the isoelectric point of modified Tomato bushy stunt virus (TBSV) particles

et al. 2017

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We present a simple method to estimate the isoelectric point (pI) of Tomato Bushy Stunt particles. We demonstrate that the combination of agarose gels with different pH buffers can be used to electrophorese the virus particles and their migration patterns can be compared. This method allows us to estimate the pI of the virus particles (wild type, wt, and genetically modified particles) and to monitor the effect of the pI of modified peptide side chains of the viral capsid subunit on the pI of the whole virus particle.

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“…For example, the serum and liver contain numerous types of nucleases that rapidly degrade or metabolize ODNs [16][17][18][19]. Hence, it is generally accepted that "protected" ODNs are more stable, efficient, and more effective than "naked" ODNs [1,13,[16][17][18][19][20][21][22][23]. The protection of the ODN may be achieved in several ways.…”

Section: Introductionmentioning

confidence: 99%