Inhibition of histamine release in vitro by a blocking factor from human serum: comparison with the iron binding proteins transferrin and lactoferrin

Abstract

Recently, we described the presence of a blocking factor (BF) in rat serum, which inhibited the histamine release from rat mast cells in vivo and in vitro. The blocking activity was demonstrated in human serum as well. Qualitative analysis of the purified preparations demonstrated a major component with an apparent molecular weight of 70,000 daltons. In human serum the blocking factor was identified as transferrin (TF) by serological and biochemical methods. BF (DEAE-peak 1) and the iron binding proteins transferrin and lactoferrin (LF) are shown to inhibit the histamine release in vitro. The dose response curves reveal that inhibition by these proteins is dependent on their degree of iron saturation. Furthermore, unlike lactoferrin, the effects of transferrin and BF (DEAE-peak 1) follow the same pattern. Their mechanism of action remains to be elucidated.

Desialylated transferrin in plasma of patients with migraine without aura

“…Moreover, we observed that treatment of control PPP with neuraminidase (which abolishes sialylation) significantly increased histamine release. Taken as a whole, these findings are in agreement with the previously reported [19,20] inhibition of histamine release by normal TF and suggest that, in MO patients, a defect in TF sialylation (occurring during or after its synthesis) reduces TF's ability to inhibit histamine release. This property may be connected (at least in part) to the previously described histamine-releasing factors (HRFs) detected in the plasma of migraine patients.…”

“…TF has been identified as one of the iron-binding proteins responsible for an inhibitory effect on histamine release from basophils and mast cells [19,20,33,34]. The dose-response curves in these studies revealed that inhibition of histamine release is dependent on the degree of TF iron saturation.…”

“…Transferrin (TF) not only transports iron in all extracellular fluids but also exerts many additional properties, including cell growth stimulation, bacteriostatic action and inhibition of histamine release from mast cells and basophils [19][20][21][22][23]. TF is a 75 kDa glycoprotein containing 679 amino acids and two glycan chains.…”

Activation of human complement by rat peritoneal mast cells and its inhibition by a rat serum factor

“…The factor was composed of two components; one at 50000-60000 and the other at 65000-70000 daltons. A similar factor was also found in normal human serum and was identified as transferrin [7,8]. In our preliminary study, relatively large amount of purified rat gamma globulin blocked the toxic effect of normal human serum, but purified rat transferrin did not (data not shown).…”

“…Krnig and his colleagues [5,6] also described a blocking factor in normal rat serum, which inhibited the passive cutaneous anaphylaxis in rat skin. Such factor was also found in normal human serum, and was identified as transferrin [7,8]. Thus, the normal sera may contain several factors, which modulate mast cell secretion, but the effects are various, differ between species, and the mechanisms have not been fully elucidated.…”