Inhibition of HHIP Promoter Methylation Suppresses Human Gastric Cancer Cell Proliferation and Migration

Zuo, Yun; Lv, Yan; Qian, Xiaolan; Wang, Shaokai; Chen, Zhipen; Jiang, Qin; Cao, Cong; Yu, Shizhu

doi:10.1159/000487875

Cited by 27 publications

(18 citation statements)

References 52 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Western blotting assay and data quantification, co-immunoprecipitation (IP) assay, MTT cell proliferation assay, Transwell migration assay, Scratch wound healing assay, and BrdU incorporation ELISA assay were described in detail in our previous studies 11 , 16 , 43 - 46 . For all Western blotting assays, the same sets of lysate samples were run in sister gels to test different proteins.…”

Section: Methodsmentioning

confidence: 99%

Gαi1 and Gαi3mediate VEGF-induced VEGFR2 endocytosis, signaling and angiogenesis

et al. 2018

Self Cite

View full text Add to dashboard Cite

VEGF binding to VEGFR2 leads to VEGFR2 endocytosis and downstream signaling activation to promote angiogenesis.Methods: Using genetic strategies, we tested the requirement of α subunits of heterotrimeric G proteins (Gαi1/3) in the process.Results: Gαi1/3 are located in the VEGFR2 endocytosis complex (VEGFR2-Ephrin-B2-Dab2-PAR-3), where they are required for VEGFR2 endocytosis and downstream signaling transduction. Gαi1/3 knockdown, knockout or dominant negative mutation inhibited VEGF-induced VEGFR2 endocytosis, and downstream Akt-mTOR and Erk-MAPK activation. Functional studies show that Gαi1/3 shRNA inhibited VEGF-induced proliferation, invasion, migration and vessel-like tube formation of HUVECs. In vivo, Gαi1/3 shRNA lentivirus inhibited alkali burn-induced neovascularization in mouse cornea. Further, oxygen-induced retinopathy (OIR)-induced retinal neovascularization was inhibited by intravitreal injection of Gαi1/3 shRNA lentivirus. Moreover, in vivo angiogenesis by alkali burn and OIR was significantly attenuated in Gαi1/3 double knockout mice. Significantly, Gαi1/3 proteins are upregulated in proliferative retinal tissues of proliferative diabetic retinopathy (PDR) patients.Conclusion: These results provide mechanistic insights into the critical role played by Gαi1/3 proteins in VEGF-induced VEGFR2 endocytosis, signaling and angiogenesis.

show abstract

Section: Methodsmentioning

confidence: 99%

Gαi1 and Gαi3mediate VEGF-induced VEGFR2 endocytosis, signaling and angiogenesis

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Epigenetic modification is an important mechanism for the occurrence and development of many metabolic diseases and cancers . DNA methylation is recognized as one of the first discovered epigenetic modifications, which can regulate gene expression by influencing chromatin structure, DNA conformation, DNA stability, and DNA‐protein action mode .…”

Section: Introductionmentioning

confidence: 99%

“…A growing number of studies have shown that DNA methylation is associated with occurrence and prognosis of cancer and might be used as a diagnostic and prognostic molecular marker . For instance, inhibition of HHIP promoter methylation could suppress human gastric cancer cell proliferation and migration; GSTM2 methylation level can be served as a potential biomarker for breast cancer development; hypermethylation of EphA5 promoter is an important marker for diagnosis and prognosis of pancreatic cancer . Our previous study found that the host immune system of liver cancer has unique DNA methylation characteristics, and significant changes of methylation in blood are expected to be noninvasive markers for early diagnosis of liver cancer .…”

Section: Introductionmentioning

confidence: 99%

Distinctive pattern of AHNAK methylation level in peripheral blood mononuclear cells and the association with HBV‐related liver diseases

Sun

Liu

et al. 2018

Cancer Medicine

View full text Add to dashboard Cite

The purpose of this study was to investigate the correlation between AHNAK methylation level in peripheral blood mononuclear cells (PBMC) and the progression of hepatitis B virus (HBV)‐related liver disease. Bioinformatics methods were applied to evaluate the AHNAK methylation level in PBMC and T cells at different stages of HBV related liver disease, to investigate the correlation between AHNAK methylation and clinical features, as well as to compare the methylation site of AHNAK in cancer tissues and adjacent tissues. Subsequently, the differentially expressed gene analysis technique was used to analyze the liver disease‐related genes and immune‐related pathways in hepatitis B patients with different pathological changes. Finally, promoter methylation and mRNA expression of AHNAK gene in liver cancer and adjacent tissues were determined by quantitative polymerase chain reaction (Q‐PCR), and the diagnostic value of AHNAK methylation level in hepatopathy was evaluated by receiver operating characteristic (ROC) curve. The promoter methylation level of AHNAK gene in PBMCs decreased with the progression of HBV‐related liver disease, and showed significant difference among the patients with various HBV‐related liver diseases (P = 0.0001). The AHNAK methylation level in PBMCs and T cells was negatively associated with age, white blood cell count, CREA, drinking, and positively associated with APTT and HbsAg. Higher mRNA expression of AHNAK was found in liver cancer tissues than that of adjacent tissues (P < 0.001), and the methylation level in PBMC decreased with the progression of hepatitis B‐related liver disease. The area under the ROC curve (ROC) was 0.883 (P < 0.001) in diagnosis of chronic hepatitis B (CHB), 0.885 (P < 0.001) in diagnosis of compensatory liver cirrhosis, 0.955 (P < 0.001) in diagnosis of decompensated liver cirrhosis, 0.981 (P < 0.001) in diagnosis of hepatocellular carcinoma. Our results revealed that AHNAK methylation level in peripheral blood decreases with the progression of hepatitis B‐related liver disease. This provided a potential differential diagnostic method for HBV‐related hepatopathies, and thus an early detective tool for liver cancer.

show abstract

“…Human OS cells (2 × 10 4 cells/well of each condition) with the applied genetic modifications were seeded on the upper surface of “Transwell” chambers (8-mm pore, BD Biosciences, San Jose, CA) [ 32 ] in serum free medium. FBS-containing complete medium was added to the lower surface of “Transwell” chambers.…”

Section: Methodsmentioning

confidence: 99%

p38γ overexpression promotes osteosarcoma cell progression

Shi

Cheng

Yin

et al. 2020

Aging

View full text Add to dashboard Cite

Osteosarcoma (OS) is the most common primary bone malignancy in the adolescent population. Recent studies demonstrate that p38 gamma (p38γ) phosphorylates retinoblastoma (Rb) to promote cyclin expression, cell-cycle entry and tumorigenesis. Studying the potential function of p38γ in human OS, we show that p38 γ mRNA and protein expression are significantly elevated in OS tissues and OS cells, whereas its expression is relatively low in normal bone tissue and in human osteoblasts/osteoblastic cells. Knockdown of p38γ in established (U2OS) and primary human OS cells potently inhibited cell growth, proliferation, migration and invasion, while promoting cell apoptosis. Furthermore, CRISPR/Cas9-induced p38γ knockout inhibited human OS cell progression in vitro . Conversely, ectopic overexpression of p38γ in primary human OS cells augmented cell growth, proliferation and migration. Signaling studies show that retinoblastoma (Rb) phosphorylation and cyclin E1/cyclin A expression were decreased following p38γ shRNA knockdown and knockout, but increased after ectopic p38γ overexpression. Collectively, these results show that p38γ overexpression promotes human OS cell progression.

show abstract

Inhibition of HHIP Promoter Methylation Suppresses Human Gastric Cancer Cell Proliferation and Migration

Cited by 27 publications

References 52 publications

Gαi1 and Gαi3mediate VEGF-induced VEGFR2 endocytosis, signaling and angiogenesis

Gαi1 and Gαi3mediate VEGF-induced VEGFR2 endocytosis, signaling and angiogenesis

Distinctive pattern of AHNAK methylation level in peripheral blood mononuclear cells and the association with HBV‐related liver diseases

p38γ overexpression promotes osteosarcoma cell progression

Contact Info

Product

Resources

About