Inhibition of herpes virus infection in oligodendrocyte cultured cells by valproic acid

Crespillo, Antonio Jesús; Praena, Beatriz; Bello-Morales, Raquel; Lerma, L.; Vázquez-Calvo, Ángela; Martín-Acebes, Miguel A.; Tabarés, E.; Sánchez‐Madrid, Francisco; López-Guerrero, José Antonio

doi:10.1016/j.virusres.2016.01.009

Cited by 21 publications

(25 citation statements)

References 58 publications

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“…Therefore, researchers have focused on remodeling or repurposing of current drugs, of which their pharmacological and toxicological properties have been already known and they are in use in clinics at present. It has been demonstrated that valproic acid and its metabolites have direct antiviral activity against several viruses, including Human Immunodeficiency Virus (HIV), Epstein-Barr virus (EBV), and Herpes Simplex virus-1 (HSV-1) [11]. An unpublished molecular repurposing study showed that valproic acid and especially its metabolite, valproic acid Co-A, might be effective against COVID-19 [12].…”

Section: Covid-19 Cytokines Immunomodulation Immunopharmacology Mood mentioning

confidence: 99%

Immunopharmacological management of COVID-19: Potential therapeutic role of valproic acid

2020

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Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre-including this research content-immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Section: Covid-19 Cytokines Immunomodulation Immunopharmacology Mood mentioning

confidence: 99%

Immunopharmacological management of COVID-19: Potential therapeutic role of valproic acid

2020

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“…Genomic DNA contamination was assessed by amplification of representative samples without real team (RT) enzyme. Real-time quantitative RT-PCR assays were performed as previously described [26,27] with primer sequences targeting the immediate early gene ICP4 and early gene polymerase (Pol) and analyzed by the Genomics Core Facility at Centro de Biología Molecular Severo Ochoa (CSIC-UAM). In order to find the most suitable genes for normalization, the stability of three candidates-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), 18S and ubiquitin (UBQ)-was analyzed with the NormFinder algorithm.…”

Section: Real-time Quantitative Rt-pcr Assaymentioning

confidence: 99%

Hsv-1 Endocytic Entry into a Human Oligodendrocytic Cell Line Is Mediated by Clathrin and Dynamin but Not Caveolin

Praena

Bello-Morales

López-Guerrero

2020

Viruses

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Endocytosis is a pathway used by viruses to enter cells that can be classified based on the proteins involved, such as dynamin, clathrin or caveolin. Although the entry of herpes simplex type 1 (HSV-1) by endocytosis has been documented in different cell types, its dependence on clathrin has not been described whereas its dependence on dynamin has been shown according to the cell line used. The present work shows how clathrin-mediated endocytosis (CME) is one way that HSV-1 infects the human oligodendroglial (HOG) cell line. Partial dynamin inhibition using dynasore revealed a relationship between decrease of infection and dynamin inhibition, measured by viral titration and immunoblot. Co-localization between dynamin and HSV-1 was verified by immunofluorescence at the moment of viral entry into the cell. Inhibition by chlorpromazine revealed that viral progeny also decreased when clathrin was partially inhibited in our cell line. RT-qPCR of immediately early viral genes, specific entry assays and electron microscopy all confirmed clathrin’s participation in HSV-1 entry into HOG cells. In contrast, caveolin entry assays showed no effect on the entry of this virus. Therefore, our results suggest the participation of dynamin and clathrin during endocytosis of HSV-1 in HOG cells.

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“…We previously showed that VPA is a potent inhibitor of the multiplication of several enveloped viruses (Vazquez-Calvo et al, 2011). VPA treatment of vesicular stomatitis virus (VSV)-infected cells reduced viral production without significantly affecting viral RNA and protein synthesis but interfering with both cell release and specific infectivity of virion particles, while VPA blocked virus entry and multiplication during West Nile virus (WNV) (Vazquez-Calvo et al, 2013) and HSV-1 infection (Crespillo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Porcine Viruses by Different Cell-Targeted Antiviral Drugs

et al. 2019

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Antiviral compounds targeting cellular metabolism instead of virus components have become an interesting issue for preventing and controlling the spread of virus infection, either as sole treatment or as a complement of vaccination. Some of these compounds are involved in the control of lipid metabolism and/or membrane rearrangements. Here, we describe the effect of three of these cell-targeting antivirals: lauryl gallate (LG), valproic acid (VPA), and cerulenin (CRL) in the multiplication of viruses causing important porcine diseases. The results confirm the antiviral action in cultured cells of LG against African swine fever virus (ASFV), foot and mouth disease virus (FMDV), vesicular stomatitis virus (VSV), and swine vesicular disease virus (SVDV), as well as the inhibitory effect of VPA and CRL on ASFV infection. Other gallate esters have been also assayed for their inhibition of FMDV growth. The combined action of these antivirals has been also tested in ASFV infections, with some synergistic effects when LG and VPA were co-administered. Regarding the mode of action of the antivirals, experiments on the effect of the time of its addition in infected cell cultures indicated that the inhibition by VPA and CRL occurred at early times after ASFV infection, while LG inhibited a late step in FMDV infection. In all the cases, the presence of the antiviral reduced or abolished the induction of virus-specific proteins. Interestingly, LG also reduced mortality and FMDV load in a mouse model. The possible use of cell-targeted antivirals against porcine diseases is discussed.

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Inhibition of herpes virus infection in oligodendrocyte cultured cells by valproic acid

Cited by 21 publications

References 58 publications

Immunopharmacological management of COVID-19: Potential therapeutic role of valproic acid

Immunopharmacological management of COVID-19: Potential therapeutic role of valproic acid

Hsv-1 Endocytic Entry into a Human Oligodendrocytic Cell Line Is Mediated by Clathrin and Dynamin but Not Caveolin

Inhibition of Porcine Viruses by Different Cell-Targeted Antiviral Drugs

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