Inhibition of Helicobacter pylori Motility by (+)-Syringaresinol from Unripe Japanese Apricot

Abstract

Helicobacter pylori was isolated from the gastric antrum of chronic gastritis patients. H. pylori is closely associated with gastritis and peptic ulcers and is even a bacterial risk factor for gastric cancer.1-4) Therefore, eradication of the bacteria and inhibition of the urease are important for the treatment of patients with gastroduodenal diseases.The standard treatment for H. pylori related disease is a combination of antimicrobial agents and anti-acid agent. 5)However, the drug resistant H. pylori against most effective antimicrobials, metronidazole and clarithromycin, is commonplace in many societies and is of particular concern as the major reason for treatment failure. [6][7][8] H. pylori is a spiral-shapes, strongly motile bacterium, and the motility is generally held to be a requirement for colonization of the stomach.9,10) Thus, one possible approach for prevention of H. pylori infection would be to inhibit the H. pylori motility.The Japanese apricot (Prunus mume SIBE. et. ZUCC.; Ume), a deciduous tree of the family Rosaceae, originated in the central and southern regions of China, and has now 400-500 varieties worldwide. The fruits of Japanese apricot are taken in foods as umeboshi, Bainiku-ekisu, pickled Japanese apricot, ume liquor and ume-based soft drinks. The fruit has been known to have various biological activities, and the fruit has been prescribed medicine for disorder of the stomach and intestines, quick recovery from fatigue, cough and diarrhea in Chinese traditional prescriptions.11,12) However, very few reports are available that proofs of components from Japanese apricot are effective against diseases.During the screening program to discover such compounds from natural products, Japanse apricot was found to show inhibitory activity against H. pylori motility. In this paper, we report the isolation and identification of inhibitor of H. pylori motility from unripe Japanese apricot. MATERIALS AND METHODSGeneral Melting point was measured on a Micro Melting Point Meter MP-500D. Optical rotation was measured on a Japan Spectroscopic Co. Ltd. DIP-1000 in CHCl 3 . The EI-MS were obtained on a JEOL the Tandem MStation JMS-700. The IR spectra were determined with a JASCO FT/IR-470 plus Fourier transform infrared spectrometer. Nuclear magnetic resonance (NMR) spectra were obtained with a JEOL FX-500 (500.00 MHz, 1 H; 125.65 MHz, 13 C) spectrometer.Materials Fresh Japanese apricot was obtained from Minabegawa Plum Research Center (Wakayama, Japan).Media and Bacterial Growth H. pylori (H. pylori ATCC43504, American type, culture collected Rokville MD, U.S.A.) was grown on blood agar plates (Trypticase soy agar supplemented with 5% sheep blood; Becton Dickinson, Tokyo, Japan) for 4 d at 37°C in a microaerophilic atmosphere (10% O 2 and 10% CO 2 ). The colonies developed were then suspended in brain heart infusion (BHI) broth (Difco) containing 10% fetal bovine serum (FBS) (Gibco, Gaithersburg, MD, U.S.A.), followed by incubation for 18 to 20 h at 37°C in a microaerophilic atmosphere. The bacterial ...

Inhibitory effects of Japanese apricot (Prunus mume Siebold et Zucc.; Ume) on Helicobacter pylori-related chronic gastritis

“…However, Miyazawa et al (2006) report that ( þ )-syringaresinol, a lignan contained in JA extract, inhibits H. pylori motility. In addition, Fujita et al (2002) found that JA extract has direct bactericidal activity against H. pylori.…”

Induction of apoptosis by ethanol extract of Prunus mume in U937 human leukemia cells through activation of caspases

“…From ancient times, various parts of P. mume have been used as a health food and a medicinal agent for the treatment of fever, cough, and intestinal disorders. In addition, recent studies have reported that P. mume possesses various pharmacological activities, including inhibition of influenza A virus and motility of Helicobacter pylori, potential sources of free radical scavengers, improvement of blood fluidity, anti-inflammation, and anti-cancer action (21)(22)(23)(24)(25)(26)(27)(28). However, the cellular and molecular mechanisms responsible for the apoptotic effects of P. mume have not yet been determined in human cancer cells.…”

“…Previous reports have suggested that P. mume exerts a wide array of pharmacological and biological activities, such as potential sources of free radical scavengers, inhibition of influenza A virus, inhibition of the motility of Helicobacter pylori, improvement of blood fluidity, and inhibition of pro-inflammatory mediators (20)(21)(22)(23)(24)(25). In addition, P. mume has been known to exert anticancer activities in several types of human cancer cells (26)(27)(28).…”

Apoptosis Induction by Methanol Extract of Prunus mume Fruits in Human Leukemia U937 Cells

“…혈구암은 임상 및 병리학적으로 급성 및 만성 혈구암으로 구분되어지는데 급성 혈구암의 경우에는 어린이나 젊은 층에서 주로 발병하지 만 만성 혈구암의 경우에는 나이가 많은 사람에게 주로 발병 하는 것으로 알려져 있다. 또한 혈구암은 골수에 위치하는 림 프구에서 유발되는 급성 림프구성 백혈병(acute lymphocytic leukemia)과 과립성 백혈구 및 단핵 백혈구에서 유발되는 골 수성 백혈병(myelogenous leukemia)으로 구분되어 진다 [1,15] [5,6,23,34,51]. 또한 매실은 많은 인체 암세포에 있어서 항암활 성을 가지는 것으로 보고되어지고 있으나 [35,36,38] [31].…”

“…또한 매실은 많은 인체 암세포에 있어서 항암활 성을 가지는 것으로 보고되어지고 있으나 [35,36,38] [31]. 최근 매실 추출물의 다양한 약리 효과가 보고되어져 오고 있으나 [5,6,23,[34][35][36]38,51] …”