Inhibition of Heat Shock Induction of Heat Shock Protein 70 and Enhancement of Heat Shock Protein 27 Phosphorylation by Quercetin Derivatives

Wang, Rongsheng E.; Kao, Jeffrey L.-F.; Hilliard, Carolyn A.; Pandita, Raj K.; Roti, Joseph L. Roti; Hunt, Clayton R.; Taylor, John‐Stephen

doi:10.1021/jm801445c

Cited by 48 publications

(53 citation statements)

References 58 publications

(130 reference statements)

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“…The activity shown by S3 fraction was very similar to quercetin, decreasing not only the heat stress inducible HSP70 protein expression (Figure 3 A, B) but also its constitutive form (data not shown). The behavior exhibited by the S2 fraction under our experimental conditions was similar to that of rutin (quercetin glycoside), a compound known to increase the HSP70 level on the plasma membrane, rising tumor cell sensitivity to be lysed by NK cells (15).…”

Section: Palliacea Fractions Regulate Intracellular Hsp70 Expressionsupporting

confidence: 57%

“…The HSP70 protein expression was evaluated by Western blot. Quercetin, a flavonoid well known to decrease HSP70 expression (15,16) was used as a positive control. The activity shown by S3 fraction was very similar to quercetin, decreasing not only the heat stress inducible HSP70 protein expression (Figure 3 A, B) but also its constitutive form (data not shown).…”

Section: Palliacea Fractions Regulate Intracellular Hsp70 Expressionmentioning

confidence: 99%

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A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression

et al. 2009

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contributed equally to this work. AbstractObjective. To evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliacea fractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines. Morphological changes, cell viability, mitochondrial membrane depolarization, nuclear staining and activity on HSP70 were analyzed. Results. The present study demonstrates that P.alliacea fractions can induce apoptosis in a mitochondria-dependent pathway and down regulate HSP70 expression in vitro. The possible compounds present in P.alliacea responsible for the described biological activities were identified by dereplication methods. Conclusion. The present study provides new knowledge on the antitumoral properties of the plant species P. alliacea described in several ethnobotanical studies.Key words: apoptosis; cancer; heat shock proteins; Petiveria alliacea. ResumenUna fracción de Petiveria alliacea induce apoptosis dependiente de la mitocondria y regula la expresión de la proteína HSP70. Objetivo. Evaluar la actividad biológica in vitro de extractos de Petiveria alliacea utilizando líneas celulares tumorales. Materiales y métodos. Las fracciones de P.alliacea fueron preparadas mediante un protocolo de purificación biodirigido. La actividad biológica fue caracterizada utilizando dos líneas celulares tumorales de origen humano, donde se analizaron los cambios morfológicos, la viabilidad celular, la actividad sobre la membrana mitocondrial y la fragmentación nuclear. Asimismo se evaluó la actividad de las fracciones sobre la expresión de la proteína HSP70. Resultados. El presente estudio muestra que las fracciones de P.alliacea inducen muerte celular por apoptosis dependiente de la mitocondria y además regulan negativamente la expresión de la proteína HSP70. Los compuestos que posiblemente son responsables de la actividad biológica presente en las fracciones de P.alliacea fueron identificados por dereplicación. Conclusión. El presente estudio contribuye a explicar en parte las propiedades antitumorales de la planta P.alliacea descritas en diversos escritos etnobotánicos.

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Section: Palliacea Fractions Regulate Intracellular Hsp70 Expressionsupporting

confidence: 57%

Section: Palliacea Fractions Regulate Intracellular Hsp70 Expressionmentioning

confidence: 99%

A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression

et al. 2009

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“…One of our initial hypotheses, that liposomal doxorubicin would reduce heat-shock protein production was disproved. Nevertheless, these fi ndings raise the possibility of potentially extending the ablation zone by eliminating heat-shock protein expression with agents such as quercetin ( 49 ).…”

Section: Experimental Studies: Increasing Stress and Apoptosis At Ablmentioning

confidence: 99%

Liposomal Doxorubicin Increases Radiofrequency Ablation–induced Tumor Destruction by Increasing Cellular Oxidative and Nitrative Stress and Accelerating Apoptotic Pathways

Solazzo

Ahmed²,

Schor-Bardach³

et al. 2010

Radiology

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Purpose:To determine if oxidative and nitrative stress and/or apoptosis contribute to increased coagulation when combining radiofrequency (RF) ablation with liposomal doxorubicin. Materials and Methods:Animal care committee approval was obtained. R3230 mammary adenocarcinomas in Fischer rats were treated with either RF ablation ( n = 43), 1 mg of intravenously injected liposomal doxorubicin ( n = 26), or combined therapy ( n = 30) and were compared with control subjects ( n = 11). A subset of animals receiving combination therapy ( n = 24) were treated in the presence or absence of N -acetylcysteine (NAC) administered 24 hours and 1 hour before RF ablation. Tumors were analyzed 2 minutes to 72 hours after treatment to determine the temporal range of response by using immunohistochemical staining of the apoptosis marker cleaved caspase-3, phosphorylated g H2AX , and Results:By 4 hours after RF ablation alone, a 0.48-mm 6 0.13 (standard deviation ) peripheral band with 57.0% 6 7.3 cleaved caspase-3 positive cells was noted at the ablation margin, whereas a 0.73-mm 6 0.18 band with 77.7% 6 6.3 positivity was seen for combination therapy ( P , .03 for both comparisons). Combination therapy caused increased and earlier staining for 4-HNE-modifi ed proteins, 8-OHdG, NT, and g H2AX with colocalization to cleaved caspase-3 staining. A rim of increased HSP70 was identifi ed peripheral to the area of cleaved caspase-3. Parameters of oxidative and nitrative stress were signifi cantly inhibited by NAC 1 hour following RF ablation, resulting in decreased cleaved caspase-3 positivity (0.28-mm 6 0.09 band of 25.9% 6 7.4 positivity vs 0.59-mm 6 0.11 band of 62.9% 6 6.0 positivity, P , .001 for both comparisons). Conclusion:Combining RF ablation with liposomal doxorubicin increases cell injury and apoptosis in the zone of increased coagulation by using a mechanism that involves oxidative and nitrative stress that leads to accelerated apoptosis.q RSNA, 2010

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“…Que, however, not only suppresses HSP70 synthesis, but also features a host of physiologic activities, including antioxidant and anti-inflammatory activity. 27 The compound also suppresses the growth of cultured cells, glycolysis, and protein kinases and ATPase. 15 Factors other than HSP70 may therefore contribute to the effects of MW and Gln on OA.…”

Section: Hsp70 Induced Therapy For Osteoarthritismentioning

confidence: 99%

Combined microwave irradiation and intraarticular glutamine administration‐induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model

Fujita

Nakagawa

Takahashi

et al. 2011

Journal Orthopaedic Research

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The objective of the present study was to investigate the effects of heat stimulation and glutamine (Gln) on the expression of extracellular matrix genes and heat shock protein 70 (HSP70) in rat articular cartilage in vivo and to determine whether HSP70 expression achieved with a combination of microwave (MW) and Gln suppresses osteoarthritis (OA) progression in a rat OA model. Stimulation at 40 W was assumed to be appropriate in the present study, and the effects of heat treatment at this intensity were evaluated. Articular cartilage was collected at 8 h after heat stimulation and/or intraarticular Gln administration, and total RNA was extracted. The expression of HSP70, aggrecan, and type II collagen was quantified using real-time RT-PCR. Cartilage samples from the OA model were subjected to hematoxylin and eosin (HE) and safranin O staining. HSP70 and aggrecan expression was greatest in a group receiving both MW and Gln. In the rat OA model, the severity of OA was significantly milder in a group receiving MW and Gln than in the control group. HSP70, stimulated by the combination of MW heat and Gln, may be involved in the suppression of OA progression. ß

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Inhibition of Heat Shock Induction of Heat Shock Protein 70 and Enhancement of Heat Shock Protein 27 Phosphorylation by Quercetin Derivatives

Cited by 48 publications

References 58 publications

A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression

A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression

Liposomal Doxorubicin Increases Radiofrequency Ablation–induced Tumor Destruction by Increasing Cellular Oxidative and Nitrative Stress and Accelerating Apoptotic Pathways

Combined microwave irradiation and intraarticular glutamine administration‐induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model

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