Inhibition of growth of mammalian cell cultures by extracts of arginine-utilizing mycoplasmas

Abstract

Mechanisms of the inhibition of growth of mammalian cell cultures caused by mycoplasmal infection were investigated by using cell-free extracts of 14 species of mycoplasmas. In four mammalian cell lines tested, the growth of two cell lines, FM3A and MDCK, was inhibited by the extracts of arginine-utilizing mycoplasmas, whereas that of the other two cell lines, Vero and LLC-MK2, was not inhibited by extracts of either arginine- or glucose-utilizing mycoplasmas. These results suggest that there are two types of cell cultures, one susceptible and the other insusceptible to arginine-utilizing mycoplasmas. In a series of experiments using FM3A cells, it was found that the growth inhibition caused by the extracts of arginine-utilizing mycoplasmas was due to removal of arginine from the medium by the action of arginine deiminase present in the extracts and that none of the metabolic products of arginine had any effect on the growth. A highly positive correlation (r = 0.96, P less than 0.01) was observed between the activity of arginine deiminase and the growth-inhibiting activity of extracts of arginine-utilizing mycoplasmas.

Incidence and distribution of argininosuccinate synthetase deficiency in human cancers

“…Areas for the tissue arrays were selected by reviewing the hematoxylin and eosin (H & E) slides. The 3 recipient blocks were con- 9 Lymphoblastic leukemia L5178Y, murine Gill and Pan, 1970 30 Lymphoblastic leukemia L5178Y, murine van Diggelen et al, 1977 36 A-9 cells, murine Jones, 1981 33 Lymphoblastic leukemia L5178Y and L1210, murine Sasaki et al, 1984 35 Breast carcinoma FM3A cells (derived from C3H Mouse mammary carcinoma) Miyazaki et al, 1990 20 Hepatoma HLE, human tongue Squamous carcinoma HSC-4, human Cervix squamous carcinoma CaSki, human Lung adenocarcinoma A549, human nasal Adenocarcinoma KB, human Glioblastoma T98G, human Sugimura et al, 1990 11 Melanoma Mewo, VMRC-MELG, A375, G361, C32TG, human melanoma B16F1, mouse T-lymphoma Jurkat, human T-cell leukemia TL-Mor and MT-2, human B-cell lymphoma Raji and Manaca, human Histiocytic lymphoma U937, human Neuroblastoma SK-N-MC, human Gonzalez and Byus, 1991 17 Skin carcinoma induced by 12-O-tetradecanoyl-phorbol-3-acetate Sugimura et al, 1992 13 Melanoma A375, G-361, Mewo, VMRC-MELG and C32TG, human Takaku et al, 1992 21 Melanoma B16, murine Hepatoma MH134, murine Colon carcinoma Colon 26, murine Fibrosarcoma Meth A, murine Sarcoma S-180, murine Leukemia L1210, murine Takaku et al, 1993 22 Hepatoma MH134, murine Colon carcinoma Colon 26, murine Sarcoma S-180, murine Leukemia L1210, murine Misawa et al, 1994 34 Hepatoma MH134, murine Fibrosarcoma Meth A, murine Takaku et al, 1995 25 Hepatoma MH134, murine Fibrosarcoma Meth A, murine Gong et al, 1999 31 Osteosarcoma SaOS, human Neuroblastoma (SH-EP and WAC2), human Retinoblastoma (Y-79), human Gong et al, 2000 32 Lymphoblastic leukemia, human Myeloid leukemia, human Kenny 1963 Lymphoblastic leukemia L5178Y, murine Kraemer 1963 and1964 Lymphoblastic leukemia L5178Y, murine Gill 1970 30 Lymphoblastic leukemia L5178Y, murine van Diggelen 1977 36 A-9 cells, murine Jones 1981 33 Lymphoblastic leukemia L5178Y and L1210, murine Sasaki 1984 35 Breast carcinoma FM3A cells (derived from C3H mouse mammary carcinoma) Miyazaki 1990 Hepatoma 32 Lymphoblastic leukemia, human Myeloid leukemia, human structed with 0.6-mm cylinders (small biopsies) and 1.0-mm cylinders (larger specimens), including 1 or 2 cylinders from each case. This procedure allowed preservation of most of the lesion in the original paraffin blocks.…”

Biofilm formation by mycoplasma species and its role in environmental persistence and survival

“…However, very little is known about their pathogenicity factors or mechanisms of persistence in the host. There are only a few characterized virulence factors of mycoplasmas; these include, in certain mycoplasma species, the production of hydrogen peroxide (Brenann & Feinstein, 1969;Miles et al, 1991), the carbohydrate capsule (Tajima et al, 1982;Almeida & Rosenbusch, 1991), the ability to scavenge arginine from host cells (Sasaki et al, 1984) and T-cell mitogens (Tu et al, 2005). It is difficult to explain how mycoplasmas manage to cause such severe and chronic infection given their paucity of virulence factors and lack of cell wall.…”

In vivo anti‐tumor activity of arginine deiminase purified from Mycoplasma arginini

“…Arginine deiminase has been purified and characterized from some strains of Mycoplasma (Weickmann and Fahrney, 1977;Kondo et al, 1990;Ohno et al, 1990). M. arginini is known to possess high activity of arginine deiminase (Sasaki et al, 1984), but its enzymological properties have not been characterized. This paper reports the properties of arginine deiminase purified from M. arginini and its in vivo and in vitro anti-tumor activities for 6 kinds of mouse ascites tumor cell lines.…”