Inhibition of growth and differentiation of osteoprogenitors in mouse bone marrow stromal cell cultures by increased donor age and glucocorticoid treatment

Chen, Theresa L.

doi:10.1016/j.bone.2004.03.019

Cited by 97 publications

(78 citation statements)

References 44 publications

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“…Currently, a definitive marker for cells of the osteogenic lineage is unknown; however, the three criteria mentioned above are widely accepted as being characteristic of osteoblasts (Andrades et al 1999;Mizuno et al 2000;Walsh et al 2003;De Jong et al 2004;Chen 2004). On exposure to osteoinductive medium, bovine MSCs, like those from other species, transformed from a fibroblastic to a cuboidal shape, thereby exhibiting osteoblastic morphology.…”

Section: Discussionmentioning

confidence: 99%

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Bosnakovski¹,

et al. 2004

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The bone marrow harbors a population of mesenchymal stem cells (MSCs) that possess the potential to differentiate into bone, cartilage, and fat, and along other tissue pathways. To date, MSCs from various species have been studied. Despite the bovine experimental model being widely used in experiments in vivo and in vitro, only a limited amount of information regarding bovine MSCs is available. The aim of this study was to isolate and induce the multilineage mesenchymal differentiation of bovine MSCs, thereby initiating further research on these cells. Bovine MSCs were isolated from eight calves, and osteogenic, chondrogenic, and adipogenic differentiation was induced by using a combination of previously reported protocols for other species. The level of differentiation was evaluated by histological examination and by analyzing the expression of tissue-specific genes by a quantitative "real time" reverse transcription/polymerase chain reaction technique. Following osteoinduction, the isolated fibroblast-like cells transformed into cuboidal cells and formed alkaline-phosphatase-positive colonies; during differentiation, these colonies transformed into mineralized nodules. In addition, osteogenesis was followed by osteocalcin and collagen type I mRNA expression. Chondrogenesis was confirmed by the demonstration of collagen type II, aggrecan, and sox9 mRNA expression in the cells stimulated by transforming growth factor β1 in monolayer culture. After being cultured in an adipogenesis-inducing medium, the MSCs responded by the accumulation of lipid vacuoles and the expression of adipocyte-specific genes. We have therefore demonstrated that cells harvested from bovine bone marrow are capable of in vitro extensive multiplication and multilineage differentiation, making them a relevant and invaluable model in the field of stem cell research.

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Section: Discussionmentioning

confidence: 99%

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Bosnakovski¹,

et al. 2004

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“…Cells were cultured in 5% CO 2 at 37°C and were harvested weekly with trypsin-EDTA solution (Invitrogen). Bone marrow stromal cells and primary mouse osteoblasts (mOB) from C57BL/6 mice were isolated as described previously (12,23). Briefly, mice were euthanized according to institutional procedure for the use of experimental animals, and the protocol was approved by the Institutional Animal Care and Use Committee of Université du Québec à Montréal.…”

Section: Methodsmentioning

confidence: 99%

Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells

Hamel¹,

Abed²,

Brissette³

et al. 2008

American Journal of Physiology-Cell Physiology

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Hamel P, Abed E, Brissette L, Moreau R. Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells. Am J Physiol Cell Physiol 294: C1021-C1033, 2008. First published February 20, 2008 doi:10.1152/ajpcell.00361.2007.-Epidemiological studies indicate that patients suffering from atherosclerosis are predisposed to develop osteoporosis. Atherogenic determinants such as oxidized low-density lipoprotein (oxLDL) particles have been shown both to stimulate the proliferation and promote apoptosis of bone-forming osteoblasts. Given such opposite responses, we characterized the oxLDL-induced hormesis-like effects in osteoblasts. Biphasic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reductive activity responses were induced by oxLDL where low concentrations (10 -50 g/ml) increased and high concentrations (from 150 g/ml) reduced the MTT activity. Cell proliferation stimulation by oxLDL partially accounted for the increased MTT activity. No alteration of mitochondria mass was noticed, whereas low concentrations of oxLDL induced mitochondria hyperpolarization and increased the cellular levels of reactive oxygen species (ROS). The oxLDL-induced MTT activity was not related to intracellular ROS levels. OxLDL increased NAD(P)H-associated cellular fluorescence and flavoenzyme inhibitor diphenyleneiodonium reduced basal and oxLDL-induced MTT activity, suggesting an enhancement of NAD(P)H-dependent cellular reduction potential. Low concentrations of oxLDL reduced cellular thiol content and increased metallothionein expression, suggesting the induction of compensatory mechanisms for the maintenance of cell redox state. These concentrations of oxLDL reduced osteoblast alkaline phosphatase activity and cell migration. Our results indicate that oxLDL particles cause hormesis-like response with the stimulation of both proliferation and cellular NAD(P)H-dependent reduction potential by low concentrations, whereas high concentrations lead to reduction of MTT activity associated with the cell death. Given the effects of low concentrations of oxLDL on osteoblast functions, oxLDL may contribute to the impairment of bone remodeling equilibrium.osteoblasts; atherosclerosis; oxysterol ELEVATED LEVELS of serum low-density lipoprotein (LDL) particles are considered as the most important atherogenic risk factor. LDL particles are thought to become atherogenic after undergoing oxidative modifications, and key roles of oxidized LDL (various oxidized species collectively designated oxLDL) in atherosclerosis have been largely reviewed by Steinberg (46). OxLDL and oxysterols have been shown to increase or decrease proliferation and trigger the apoptosis process depending on the cell types related to vasculature alterations, nature of oxLDL and oxysterols, and on the concentrations used (52), thus supporting numerous deleterious effects that sustain the development of atherosclerosis. Also, a number of clinical studies suggest an association between cardiovascular disease and the develo...

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“…However, the differentiation factors, including dexamethasone and 1α,25-dihydroxyvitamin D3, may inhibit cell proliferation (Walsh et al, 2001;Atmani et al, 2003;Chen, 2004;Kim et al, 2006) for both BMSCs and osteoblasts. Moreover, it is unknown how the OM affects differentiated MSCs.…”

Section: Introductionmentioning

confidence: 99%

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Wang

Singh

Bonewald

et al. 2009

J Tissue Eng Regen Med

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Human umbilical cord mesenchymal stromal cells (hUCMSCs) have recently shown the capacity to differentiate into multiple cell lineages in all three embryonic germ layers. The osteogenic differentiation of hUCMSCs in monolayer culture has been reported, while the differentiation in three-dimensional biomaterials has not yet been reported for tissue-engineering applications. Thus, the aim of this study was to evaluate the feasibility of using hUCMSCs for bone tissue engineering. hUCMSCs were cultured in poly(L-lactic acid) (PLLA) scaffolds in osteogenic medium (OM) for 3 weeks, after which the scaffolds were exposed to several different media, including the OM, a mineralization medium (MM) and the MM with either 10 or 100 ng/ml insulin-like growth factor (IGF)-1. The osteogenic differentiation was confirmed by the up-regulation of Runx2 and OCN, calcium quantification and bone histology. Switching from the OM to the MM promoted collagen synthesis and calcium content per cell, while continuing in the OM retained more cells in the constructs and promoted higher osteogenic gene expression. The addition of IGF-1 into the MM had no effect on cell proliferation, differentiation and matrix synthesis. In conclusion, hUCMSCs show significant potential for bone tissue engineering and culturing in the OM throughout the entire period is beneficial for osteogenic differentiation of these cells.

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Inhibition of growth and differentiation of osteoprogenitors in mouse bone marrow stromal cell cultures by increased donor age and glucocorticoid treatment

Cited by 97 publications

References 44 publications

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

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