Inhibition of goby posterior intestinal NaCl absorption by natriuretic peptides and by cardiac extracts

Abstract

Natriuretic peptides abolish active Na+ and Cl- absorption across the posterior intestine of the euryhaline goby Gillichthys mirabilis. Inhibition by eel and human natriuretic peptides is dose-dependent with the following sequence of potencies based on experimentally determined ID50 values for inhibition of short-circuit current: eel ventricular natriuretic peptide (78 nmol.l-1), eel atrial natriuretic peptide (156 nmol.l-1), human brain natriuretic peptide (326 nmol.l-1), human alpha atrial natriuretic peptide (1.05 mumol.l-1), and eel C-type natriuretic peptide (75 mumol.l-1). Natriuretic peptides also significantly increase transcellular conductance. The observed sequence of natriuretic peptide potencies is suggestive of cellular mediation by GC-A-type NP-R1 receptors in this tissue; as expected for guanylyl-cyclase-coupled NP-R1 receptors, cyclic GMP mimics the action of natriuretic peptides on the goby intestine. Crude aqueous extracts of goby atrium and ventricle inhibited short circuit current and increased tissue conductance in a dose-dependent manner. Ventricular extract was more potent than atrial extract on both a per organ and per milligram basis.

The natriuretic peptide system in eels: a key endocrine system for euryhalinity?

“…In the intestine, ANP potently inhibits NaCl absorption through the inhibition of Na-K-2Cl cotransporter in the winter flounder, Pseudopleuronectes americanus (65). Similar results are obtained in the goby, Gillichthys mirabilis (50). ANP stimulates chloride secretion (short-circuit current) in the isolated opercular epithelium of killifish (75).…”

The natriuretic peptide system in eels: a key endocrine system for euryhalinity?

“…In the intestine, ANP potently inhibits NaCl absorption through the inhibition of Na-K-2Cl cotransporter in the winter flounder, Pseudopleuronectes americanus (65). Similar results are obtained in the goby, Gillichthys mirabilis (50). ANP stimulates chloride secretion (short-circuit current) in the isolated opercular epithelium of killifish (75).…”

Some Factors Affecting Drinking Behavior and Their Interactions in Seawater-Acclimated Eels, Anguilla japonica

“…Our current results provided the first direct evidence to show that ANP profoundly inhibits intestinal Na + absorption in vivo except in the dog where ANP inhibits it in the jejunum (Matsushita et al, 1991). There have been in vitro studies showing the inhibitory effect of ANP on short-circuit current in teleost intestine (O'Grady et al, 1985;Ando et al, 1992;Loretz, 1996). In the flounder, ANP inhibits Na et al, 1985), but in mammals, the ANP effect is mediated by Na + / glucose cotransporters (Gonzalez-Bosc et al, 2000).…”

Characterisation of C-type natriuretic peptide receptors in the gill of dogfish Triakis scyllia

“…Therefore the contribution of the intestine to elasmobranch osmoregulation may not be so large. ANP potently inhibits intestinal ion and water transport in mammals and teleost fish (O'Grady et al 1985, Matsushita et al 1991, Ando et al 1992, Loretz 1996. The presence of dense GC-coupled CNP receptors in the dogfish intestine, suggested in this study, is intriguing.…”

The natriuretic peptide system in eels: a key endocrine system for euryhalinity?

“…In the intestine, ANP potently inhibits NaCl absorption through the inhibition of Na-K-2Cl cotransporter in the winter flounder, Pseudopleuronectes americanus (65). Similar results are obtained in the goby, Gillichthys mirabilis (50). ANP stimulates chloride secretion (short-circuit current) in the isolated opercular epithelium of killifish (75).…”

Some Factors Affecting Drinking Behavior and Their Interactions in Seawater-Acclimated Eels, Anguilla japonica

“…Our current results provided the first direct evidence to show that ANP profoundly inhibits intestinal Na + absorption in vivo except in the dog where ANP inhibits it in the jejunum (Matsushita et al, 1991). There have been in vitro studies showing the inhibitory effect of ANP on short-circuit current in teleost intestine (O'Grady et al, 1985;Ando et al, 1992;Loretz, 1996). In the flounder, ANP inhibits Na et al, 1985), but in mammals, the ANP effect is mediated by Na + / glucose cotransporters (Gonzalez-Bosc et al, 2000).…”

Characterisation of C-type natriuretic peptide receptors in the gill of dogfish Triakis scyllia

“…Therefore the contribution of the intestine to elasmobranch osmoregulation may not be so large. ANP potently inhibits intestinal ion and water transport in mammals and teleost fish (O'Grady et al 1985, Matsushita et al 1991, Ando et al 1992, Loretz 1996. The presence of dense GC-coupled CNP receptors in the dogfish intestine, suggested in this study, is intriguing.…”

The natriuretic peptide system in eels: a key endocrine system for euryhalinity?

“…In the intestine, ANP potently inhibits NaCl absorption through the inhibition of Na-K-2Cl cotransporter in the winter flounder, Pseudopleuronectes americanus (65). Similar results are obtained in the goby, Gillichthys mirabilis (50). ANP stimulates chloride secretion (short-circuit current) in the isolated opercular epithelium of killifish (75).…”

Some Factors Affecting Drinking Behavior and Their Interactions in Seawater-Acclimated Eels, Anguilla japonica

“…Our current results provided the first direct evidence to show that ANP profoundly inhibits intestinal Na + absorption in vivo except in the dog where ANP inhibits it in the jejunum (Matsushita et al, 1991). There have been in vitro studies showing the inhibitory effect of ANP on short-circuit current in teleost intestine (O'Grady et al, 1985;Ando et al, 1992;Loretz, 1996). In the flounder, ANP inhibits Na et al, 1985), but in mammals, the ANP effect is mediated by Na + / glucose cotransporters (Gonzalez-Bosc et al, 2000).…”

Characterisation of C-type natriuretic peptide receptors in the gill of dogfish Triakis scyllia

“…Therefore the contribution of the intestine to elasmobranch osmoregulation may not be so large. ANP potently inhibits intestinal ion and water transport in mammals and teleost fish (O'Grady et al 1985, Matsushita et al 1991, Ando et al 1992, Loretz 1996. The presence of dense GC-coupled CNP receptors in the dogfish intestine, suggested in this study, is intriguing.…”