Inhibition of Glutamine Synthetase and Quantitative Changes of Free Amino Acids in Shoots of Bialaphostreated Japanese Barnyard Millet

Tachibana, Kunitaka; Watanabe, Tomoaki; Sekizawa, Yasuharu; Takematsu, Tetsuo

doi:10.1584/jpestics.11.27

Cited by 66 publications

(27 citation statements)

References 1 publication

(1 reference statement)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to the inhibition of GS, non-tolerant plant cells accumulate large amounts of toxic ammonia produced by nitrate assimilation and photorespiration (Tachibana et al, 1986) and the level of available glutamine drops (Sauer et al, 1987). Damage of cell membranes and inhibition of photosynthesis are followed by plant cell death.…”

Section: Section I -Biochemistry and Physiology Of The Herbicide In Nmentioning

confidence: 99%

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Barnett

Culpepper²,

York

et al. 2015

Weed technol.

View full text Add to dashboard Cite

Glyphosate-resistant (GR) weeds are most challenging for growers to control in cotton in the midsouth region of the United States. As a result, growers in these states are transitioning from a glyphosate-based system to a glufosinate-based system. A field study was conducted in 2010 and 2011 in Georgia, North Carolina, and Tennessee to determine the effect of repeated glufosinate applications to WideStrike cotton applied at various application timings. One to three glufosinate applications at 594 g ai ha−1were applied to cotton at the two-leaf, seven-leaf, bloom, or 2 wk after bloom stages. Visual estimates of crop injury were evaluated after each application, in addition to crop height, development, and yield. Crop injury after each glufosinate application typically ranged from 3 to 11%, with higher injury observed where multiple glufosinate applications were made. Crop height differences were noted after the bloom and 2 wk after bloom applications. Treatments that had one, two, or three glufosinate applications had reduced crop height when compared with the weed-free control. Node above cracked boll ratings also indicated that glufosinate treatments may have stressed the cotton. Ultimately, yield was reduced where three glufosinate treatments were applied to WideStrike cotton. In addition, one glufosinate application at the bloom stage or two glufosinate applications at the bloom and 2 wk after bloom stages reduced yield when compared with the weed-free control. Although it is a legal application, growers should use caution when applying glufosinate to WideStrike cotton because the manufacturer of glufosinate and the manufacturer of WideStrike cotton do not recommend this application. However, where GR Palmer amaranth is present, these applications may be necessary.

show abstract

Section: Section I -Biochemistry and Physiology Of The Herbicide In Nmentioning

confidence: 99%

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Barnett

Culpepper²,

York

et al. 2015

Weed technol.

View full text Add to dashboard Cite

show abstract

“…Two different strategies are found in nature to overcome these limitations. Phosphinothricin mimics the tetrahedral intermediate generated in Gln synthase (13,14) by using a monoanionic phosphinate group containing two P-C bonds (Fig. 1A).…”

mentioning

confidence: 99%

Characterization and structure of DhpI, a phosphonate O -methyltransferase involved in dehydrophos biosynthesis

Lee

Bae

Kuemin

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Phosphonate natural products possess a range of biological activities as a consequence of their ability to mimic phosphate esters or tetrahedral intermediates formed in enzymatic reactions involved in carboxyl group metabolism. The dianionic form of these compounds at pH 7 poses a drawback with respect to their ability to mimic carboxylates and tetrahedral intermediates. Microorganisms producing phosphonates have evolved two solutions to overcome this hurdle: biosynthesis of monoanionic phosphinates containing two P-C bonds or esterification of the phosphonate group. The latter solution was first discovered for the antibiotic dehydrophos that contains a methyl ester of a phosphonodehydroalanine group. We report here the expression, purification, substrate scope, and structure of the O-methyltransferase from the dehydrophos biosynthetic gene cluster. The enzyme utilizes S-adenosylmethionine to methylate a variety of phosphonates including 1-hydroxyethylphosphonate, 1,2-dihydroxyethylphosphonate, and acetyl-1-aminoethylphosphonate. Kinetic analysis showed that the best substrates are tripeptides containing as C-terminal residue a phosphonate analog of alanine suggesting the enzyme acts late in the biosynthesis of dehydrophos. These conclusions are corroborated by the X-ray structure that reveals an active site that can accommodate a tripeptide substrate. Furthermore, the structural studies demonstrate a conformational change brought about by substrate or product binding. Interestingly, the enzyme has low substrate specificity and was used to methylate the clinical antibiotic fosfomycin and the antimalaria clinical candidate fosmidomycin, showing its promise for applications in bioengineering.antibiotics | bioengineering | conformational change | X-ray crystallography | domain-swap

show abstract

“…In the work by Tachibana et al [7], about 30 to 100 times' increase in ammonium content after the PPT treatment of several plant species and a close correlation between herbicide activity and ammonium levels were demonstrated. At the same time, addition of glutamine did not affect substantially the herbicide activity of PPT [15]. On the other hand, addition of glutamine and glutamate to PPT reduced the photosynthesis inhibition in Sinapis alba; that is, the NH 4 + accumulation cannot be the primary cause for photosynthesis inhibition by PPT [8].…”

Section: Discussionmentioning

confidence: 84%

“…However our research demonstrated no correlation between ammonium accumulation in transgenic birch lines treated with 2.5 Lha ) survived. For assessment of plant sensitivity to PPT, such parameter as decrease of fresh [7,15] or dry weight [13] was used. Since the "Basta" herbicide is also used as desiccant, we decided to use index of desiccation of foliar tissue.…”

Section: Journal Of Botanymentioning

confidence: 99%

Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

2015

View full text Add to dashboard Cite

Transformation of plants with genes encoding a glutamine synthetase (GS), a key nitrogen metabolism enzyme, is usually used to increase productivity. However, overexpression of these genes may increase resistance to phosphinothricin (PPT) that irreversibly inhibits GS causing ammonium accumulation in plant tissues. Transgenic plants of two birch (Betula pubescens) genotypes expressing a pine cytosolic GS gene were used for studying the PPT effect on trees. Two control and 8 transgenic lines were treated with herbicide "Basta" at dose equivalent to 2.5 and 5 Lha −1 . Necrosis and abscission of leaves occurred irrespective of a transgenic status or the treatment dose. Ammonium content in leaf tissue in 3 days after the 5 Lha −1 treatment was substantially increased in all plants, 3.2-16.0 times depending on line. After the 2.5 Lha −1 treatment, ammonium content in three transgenic lines was not different from that in control variant sprayed with water. The herbicide treatment caused more prominent desiccation in the bp3f1 genotype nontransgenic plants as compared to transgenic plants, but not in the bp4a genotype. Lack of correlation between ammonium levels and survival of transgenic plants suggests that ammonium toxicity is not a main reason for the birch plant death after the PPT treatment.

show abstract

Inhibition of Glutamine Synthetase and Quantitative Changes of Free Amino Acids in Shoots of Bialaphostreated Japanese Barnyard Millet

Cited by 66 publications

References 1 publication

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Evaluation of WideStrike Cotton Response to Repeated Applications of Glufosinate at Various Application Timings

Characterization and structure of DhpI, a phosphonate O -methyltransferase involved in dehydrophos biosynthesis

Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

Contact Info

Product

Resources

About