Inhibition of Gasdermin D blocks the formation of NETs and protects acute pancreatitis in mice

Han, Fei; Chen, Hao; Chen, Lin; Yuan, Chunping; Shen, Qinhao; Lu, Guotao; Chen, Weiwei; Gong, Weijuan; Ding, Yanbing; Gu, Aidong; Tao, Li

doi:10.1016/j.bbrc.2023.02.082

Cited by 1 publication

(1 citation statement)

References 21 publications

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“…On the other hand, Baicalin has been found to modulate miR-15a, offering relief from AP progression [4]. Additionally, irisin has been reported to reduce AP progression in mice by inhibiting the formation of neutrophil extracellular traps [5]. Gentiana scabra, a traditional medicinal plant, has been used to treat various diseases, and related research has revealed that its extract, gentianine (GTN), can regulate inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Gentianine facilitates proliferation and inhibits inflammation and oxidative stress in caerulein-triggered acute pancreatitis cell model

2024

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Acute pancreatitis (AP) is a life-threatening inflammatory condition of the pancreas. Gentianine (GTN), derived from Gentiana scabra, has been demonstrated to suppress inflammation in various diseases. Nonetheless, the specific effects and related pathways through which GTN influences AP progression remain unclear. Herein, we aimed to elucidate the regulatory effects of GTN on AP. Our results confirmed that the reduction in cell proliferation induced by caerulein could be reversed after GTN treatment (25 µM, 50 µM and 100 µM). Moreover, while caerulein treatment increased cell apoptosis, subsequent GTN treatment reduced this effect, as well as the inflammation provoked by caerulein. Furthermore, activation of the Toll-like receptor 4 (TLR4)/nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) pathway induced by caerulein was counteracted by GTN administration. In summary, this research revealed for the first time that GTN promotes cell proliferation and reduces inflammation and oxidative stress in AR42J cells in a caerulein-induced AP cell model, suggesting that GTN holds promise as an effective therapeutic agent for AP.

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Section: Introductionmentioning

confidence: 99%

Gentianine facilitates proliferation and inhibits inflammation and oxidative stress in caerulein-triggered acute pancreatitis cell model

2024

View full text Add to dashboard Cite

show abstract

Inhibition of Gasdermin D blocks the formation of NETs and protects acute pancreatitis in mice

Cited by 1 publication

References 21 publications

Gentianine facilitates proliferation and inhibits inflammation and oxidative stress in caerulein-triggered acute pancreatitis cell model

Gentianine facilitates proliferation and inhibits inflammation and oxidative stress in caerulein-triggered acute pancreatitis cell model

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