Inhibition of fibroblast growth factor-inducible 14 attenuates experimental tubulointerstitial fibrosis and profibrotic factor expression of proximal tubular epithelial cells

Luo, Mai; Liu, Mengmeng; Liu, Wei; Cui, Xiaojie; Zhai, Siyue; Gu, Hanjiang; Wang, Huixia; Wu, Kunyi; Zhang, Wen; Li, Ké; Xia, Yumin

doi:10.1007/s00011-021-01455-0

Cited by 3 publications

(10 citation statements)

References 30 publications

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“…We collected rabbit forebrain tissue samples from the three experimental groups of rabbit pups at postnatal days 3, 7 and 14 (Georgiadis et al, 2008;Vinukonda et al, 2010Vinukonda et al, , 2019. Soon, after rabbit pups were put to death, their brains were quickly removed and cut into a single 3 mm coronal slice at the mid-septal nucleus (using a brain matrix slicer, Cat #BSRAS001-1, Zivic Instruments, Pittsburg, PA, USA) starting from the cranial end of the frontal lobe.…”

Section: Assignment Of Rabbit Pups To Experimental Groups Initial Tissue Collection and Processing For Endpoint Studiesmentioning

confidence: 99%

“…Immunohistochemical staining was performed as we previously described (Braun et al, 2007;Vinukonda et al, 2010). Briefly, the tissue was fixed in 4% paraformaldehyde in phosphate buffered saline (0.1 M PBS; pH: 7.4) for 18 h followed by cryoprotection by immersing slices in 15% sucrose in 0.1 M PBS for 24 h and then another 24 h in 30% sucrose.…”

Section: Immunohistochemistry (Ihc) and Image Analysis For Aqp1 Expressionmentioning

confidence: 99%

“…Using the coronal brain slices at the level of the mid-septal nucleus (Vinukonda et al, 2010(Vinukonda et al, , 2019, approximately 200 mg of frozen tissue was homogenized in 500 µl of 1× lysis buffer from 2× stock buffer provided by manufacturer (CODE #EL-lysis, Ray Biotech). The tissue homogenates were made using Minilys homogenizer (Bertin Technologies, MD, USA) and Precellys lysing kit for soft tissue homogenizing ceramic beads (1.4 mm, part #91-PCS-CK14; Bertin Technologies, MD, USA).…”

Section: Enzyme-linked Immunosorbent Assay For Mmp-9 Expression In the Dissected Tissue Lysatementioning

confidence: 99%

“…Since the brain hemorrhage and resultant reactive gliosis is non-uniform around the ventricle (subventricular and periventricular white matter) we counted all positive cells in the entire subventricular zone SVZ and periventricular zone (PVZ) area. The cell counting on fixed sections was done as described previously (Vinukonda et al, 2010). Two investigators (blinded for experimental groups) determined the cell density using Image-J software with grid.…”

Section: Quantification Of Normal and Reactive Astrocyte Cell Densitymentioning

confidence: 99%

“…The TaqMan gene expression was performed using ABI quanta studio (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (Vinukonda et al, 2010(Vinukonda et al, , 2019. The mRNA transcript levels were determined by two-step quantitative real time TaqMan PCR using the TaqMan chemistry reaction mix purchased from Roche (Cat #04913850001, Roche, Indianapolis, IN, USA).…”

Section: Taqman Gene Expression Assaymentioning

confidence: 99%

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Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage

Purohit

Finkel

Malfa

et al. 2021

Front. Cell. Neurosci.

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Intraventricular hemorrhage (IVH) is a severe complication of preterm birth associated with cerebral palsy, intellectual disability, and commonly, accumulation of cerebrospinal fluid (CSF). Histologically, IVH leads to subependymal gliosis, fibrosis, and disruption of the ependymal wall. Importantly, expression of aquaporin channels 1 and 4 (AQP1 and AQP4) regulating respectively, secretion and absorption of cerebrospinal fluids is altered with IVH and are associated with development of post hemorrhagic hydrocephalus. Human cord blood derived unrestricted somatic stem cells (USSCs), which we previously demonstrated to reduce the magnitude of hydrocephalus, as having anti-inflammatory, and beneficial behavioral effects, were injected into the cerebral ventricles of rabbit pups 18 h after glycerol-induced IVH. USSC treated IVH pups showed a reduction in ventricular size when compared to control pups at 7 and 14 days (both, P < 0.05). Histologically, USSC treatment reduced cellular infiltration and ependymal wall disruption. In the region of the choroid plexus, immuno-reactivity for AQP1 and ependymal wall AQP4 expression were suppressed after IVH but were restored following USSC administration. Effects were confirmed by analysis of mRNA from dissected choroid plexus and ependymal tissue. Transforming growth factor beta (TGF-β) isoforms, connective tissue growth factor (CTGF) and matrix metalloprotease-9 (MMP-9) mRNA, as well as protein levels, were significantly increased following IVH and restored towards normal with USSC treatment (P < 0.05). The anti-inflammatory cytokine Interleukin-10 (IL-10) mRNA was reduced in IVH, but significantly recovered after USSC injection (P < 0.05). In conclusion, USSCs exerted anti-inflammatory effects by suppressing both TGF-β specific isoforms, CTGF and MMP-9, recovered IL-10, restored aquaporins expression towards baseline, and reduced hydrocephalus. These results support the possibility of the use of USSCs to reduce IVH consequences in prematurity.

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Section: Assignment Of Rabbit Pups To Experimental Groups Initial Tissue Collection and Processing For Endpoint Studiesmentioning

confidence: 99%

Section: Immunohistochemistry (Ihc) and Image Analysis For Aqp1 Expressionmentioning

confidence: 99%

Section: Enzyme-linked Immunosorbent Assay For Mmp-9 Expression In the Dissected Tissue Lysatementioning

confidence: 99%

Section: Quantification Of Normal and Reactive Astrocyte Cell Densitymentioning

confidence: 99%

Section: Taqman Gene Expression Assaymentioning

confidence: 99%

See 3 more Smart Citations

Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage

Purohit

Finkel

Malfa

et al. 2021

Front. Cell. Neurosci.

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Renal fibrosis in type 2 cardiorenal syndrome: An update on mechanisms and therapeutic opportunities

Zhang

Wang

et al. 2023

Biomedicine & Pharmacotherapy

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Emerging role of TWEAK‐Fn14 axis in lupus, a disease related to autoimmunity and fibrosis

Wei

Jing

et al. 2021

Int J of Rheum Dis

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Systemic lupus erythematosus (SLE) is one of the complex disorders in human beings. It is characterized by much production of autoantibodies, which deposit in organs, such as the kidney. 1 Lupus nephritis (LN) is a characteristic complication in SLE, where there is a dysregulated inflammatory environment in the kidney.Excessive inflammatory cell infiltration, pro-inflammatory cytokines and chemokines accumulation promote a series of damages in the kidney, such as fibrosis. Finding out the reasons related to kidney damage in lupus, and targeting the damage is good for reversing lupus.Being a member of the tumor necrosis factor (TNF) ligand family, TNF-like weak inducer of apoptosis (TWEAK) is a multifunctional pro-inflammatory cytokine. It was first described as an inducer of apoptosis in transformed cell lines, 2 then, it was reported to correlate with a pathogenic role in inflammation. Human TWEAK gene was located at chromosome 17p13.1, which encoded a 249 amino acid type II transmembrane glycoprotein with 30 kD. TWEAK is expressed in different immune cells, tissues, organs, such as monocytes, dendritic cells, T cells as well as heart, colon and kidney. 3 Fibroblast growth factor-inducible 14 (Fn14) is the only receptor for TWEAK. Fn14 is expressed in a variety of resident cells, especially under inflammatory conditions. 4,5 TWEAK binds to Fn14, activates

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Inhibition of fibroblast growth factor-inducible 14 attenuates experimental tubulointerstitial fibrosis and profibrotic factor expression of proximal tubular epithelial cells

Cited by 3 publications

References 30 publications

Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage

Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage

Renal fibrosis in type 2 cardiorenal syndrome: An update on mechanisms and therapeutic opportunities

Emerging role of TWEAK‐Fn14 axis in lupus, a disease related to autoimmunity and fibrosis

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